Current Protein Identity：Q9Y7J4
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3DWL Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit Deposited 2008-07-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；750 mM ammonium sulfate
50 mM sodium citrate
7 % glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.78 Å R-free 0.344 |
| 3DWL Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit Deposited 2008-07-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain J
1–174（174 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；750 mM ammonium sulfate
50 mM sodium citrate
7 % glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.78 Å R-free 0.344 |
| 6W17 Structure of Dip1-activated Arp2/3 complex with nucleated actin filament Deposited 2020-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric（17） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6W18 Structure of S. pombe Arp2/3 complex in inactive state Deposited 2020-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；4 uL of sample was applied to freshly glow-discharged grid. Excess sample was manually blotted off with a dry Whatman No.1 filter paper for 5-7 seconds. Immediately after the blotting step the sample containing grid was rapidly vitrified by plunge freezing into liquid ethane.
|
Resolution 4.20 Å |
| 8E9B Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction Deposited 2022-08-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | MG MAGNESIUM ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8UXW Arp2/3 branch junction complex, ADP state Deposited 2023-11-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.70 Å |
| 8UXX Arp2/3 branch junction complex, BeFx state Deposited 2023-11-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain E
1–174（174 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 BEF BERYLLIUM TRIFLUORIDE ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.20 Å |