Acetyl-CoA decarbonylase/synthase (ACDS), natively purified from Methanosarcina acetivorans

{
  "code": "SASD2C2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Acetyl-CoA decarbonylase/synthase (ACDS) from Methanosarcina acetivorans",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-07-01",
    "released_date": "2026-07-29"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASD2C2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASD2C2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASD2C2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASD2C2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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        {
          "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit alpha 2",
          "short_name": "CdhA",
          "sequence": ">Q8TJC6\r\nMSKLTTGSFSIEDLESVQITINNIVGAAKEAAEEKAKELGPMGPTAMAGLASYRSWNLLL\r\nLDRYEPVLTPMCDQCCYCTYGPCDLSGNKRGACGIDMAGQTGREFFLRVITGTACHAAHG\r\nRHLLDHVIEVFGEDLPLNLGESNVLTPNVTICTGLSPKTLGECRAPMEYVEEQLTQLLAT\r\nIHAGQESAEIDYDSKALFSGSLDHVGMEVSDIAQVSAYDFPKADPEAPLIEIGMGSIDKS\r\nKPLIVAIGHNVAGVTYIMDYMEENNLTDKMEIAGLCCTAFDMTRYKEADRRAPYAKIVGS\r\nLAKELKVIRSGMPDVIVVDEQCVRGDVLSESQKLKIPVIASNEKIMMGLPDRTDADVDSI\r\nVEEIKSGAIPGCVMLDYDKLGELIPKIAEVMAPIRDAEGITAIPTDEEFKVYIDKCVKCG\r\nECMLACPEELDIPEALEYAAKGSYEYLEALHDVCIGCRRCEQVCKKEIPILNVLEKAAQK\r\nSISEEKGWVRSGRGQASDAEIRKEGLNLVMGTTPGIIAIIGCPNYPAGTKDVYLIAEEFL\r\nKRNYLLAVSGCSAMDIGMFKDEDGKTLYEKYPGTFAGGGLLNTGSCVSNAHISGAAEKVA\r\nGIFAQRTLAGNLAEIADYTLNRVGACGLAWGAYSQKAASIGTGCNIYGIPAVLGPHSSKY\r\nRRALIAKNYDESKWKVYDGRDGSEMTIPPAPEFLLTTAETWQEAIPMMAKACIRPSDNNM\r\nGRSIKLTHWMELSKKYLGVEPEDWWKFVRNEADLPLAKREELLKRLEAEHGWEIDWKRKK\r\nIISGPKIKFDVSAQPTNLKRLCKEA",
          "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)",
          "uniprot_code": "Q8TJC6",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MSKLTTGSFSIEDLESVQITINNIVGAAKEAAEEKAKELGPMGPTAMAGLASYRSWNLLL\nLDRYEPVLTPMCDQCCYCTYGPCDLSGNKRGACGIDMAGQTGREFFLRVITGTACHAAHG\nRHLLDHVIEVFGEDLPLNLGESNVLTPNVTICTGLSPKTLGECRAPMEYVEEQLTQLLAT\nIHAGQESAEIDYDSKALFSGSLDHVGMEVSDIAQVSAYDFPKADPEAPLIEIGMGSIDKS\nKPLIVAIGHNVAGVTYIMDYMEENNLTDKMEIAGLCCTAFDMTRYKEADRRAPYAKIVGS\nLAKELKVIRSGMPDVIVVDEQCVRGDVLSESQKLKIPVIASNEKIMMGLPDRTDADVDSI\nVEEIKSGAIPGCVMLDYDKLGELIPKIAEVMAPIRDAEGITAIPTDEEFKVYIDKCVKCG\nECMLACPEELDIPEALEYAAKGSYEYLEALHDVCIGCRRCEQVCKKEIPILNVLEKAAQK\nSISEEKGWVRSGRGQASDAEIRKEGLNLVMGTTPGIIAIIGCPNYPAGTKDVYLIAEEFL\nKRNYLLAVSGCSAMDIGMFKDEDGKTLYEKYPGTFAGGGLLNTGSCVSNAHISGAAEKVA\nGIFAQRTLAGNLAEIADYTLNRVGACGLAWGAYSQKAASIGTGCNIYGIPAVLGPHSSKY\nRRALIAKNYDESKWKVYDGRDGSEMTIPPAPEFLLTTAETWQEAIPMMAKACIRPSDNNM\nGRSIKLTHWMELSKKYLGVEPEDWWKFVRNEADLPLAKREELLKRLEAEHGWEIDWKRKK\nIISGPKIKFDVSAQPTNLKRLCKEA",
          "mw": 87.954,
          "total_mw": 703.632,
          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2",
          "short_name": "CdhB",
          "sequence": ">Q8TJC5\r\nMVDTTKNTKLFTSYGVTTSKTTTPEIAAKLISKAKRPLLVVGTKVLDPELLDRAVKIAQK\r\nANIPIAATGSSMPGFVGKDVDAKYINLHQLGFYVTDPNWPGLDGNGTYDTLIVLGHIKYY\r\nINQVLSGTKNFSTVKAIAIERNYIQNATMSFGNLSKADHYAALDELIDAL",
          "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)",
          "uniprot_code": "Q8TJC5",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MVDTTKNTKLFTSYGVTTSKTTTPEIAAKLISKAKRPLLVVGTKVLDPELLDRAVKIAQK\nANIPIAATGSSMPGFVGKDVDAKYINLHQLGFYVTDPNWPGLDGNGTYDTLIVLGHIKYY\nINQVLSGTKNFSTVKAIAIERNYIQNATMSFGNLSKADHYAALDELIDAL",
          "mw": 18.466,
          "total_mw": 147.728,
          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit beta 2",
          "short_name": "CdhC",
          "sequence": ">Q8TJC4\r\nMAEFPFEISPMFEGERVRKEGMFVELGGPKSLGLELVRAKPMDEIEDDKVTIVGPDLKEM\r\nEEGKTYPWAMIFNIGGELVEPDLESVVERRVHDFINYCQGIMHLNQRYDVWMRVSKDTAA\r\nKMDSFEPFGQAVMMLFKTELPFIEKMQVTFYTEQAEVEKQLEEAKAIFKARDERTKDLHD\r\nEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFEITKGDLI\r\nDAEKGEYTGVNDIAKKLSAGEFDKIKLHSFFDAPHTSCGCFEVVGFYIPEVDGIGWVNRE\r\nYQGMAPNGIGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKDK\r\nIAETIPEDLKDKIATENDATDIESLKAFLQEKGHPVVATWAAAEEEEEEEEEEEEEVAVA\r\nAAPMMMPAAGFQMPAMPMMSGGSSGGIKLTFKNAKITIDKMIISEKKEKK",
          "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)",
          "uniprot_code": "Q8TJC4",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MAEFPFEISPMFEGERVRKEGMFVELGGPKSLGLELVRAKPMDEIEDDKVTIVGPDLKEM\nEEGKTYPWAMIFNIGGELVEPDLESVVERRVHDFINYCQGIMHLNQRYDVWMRVSKDTAA\nKMDSFEPFGQAVMMLFKTELPFIEKMQVTFYTEQAEVEKQLEEAKAIFKARDERTKDLHD\nEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFEITKGDLI\nDAEKGEYTGVNDIAKKLSAGEFDKIKLHSFFDAPHTSCGCFEVVGFYIPEVDGIGWVNRE\nYQGMAPNGIGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKDK\nIAETIPEDLKDKIATENDATDIESLKAFLQEKGHPVVATWAAAEEEEEEEEEEEEEVAVA\nAAPMMMPAAGFQMPAMPMMSGGSSGGIKLTFKNAKITIDKMIISEKKEKK",
          "mw": 52.426,
          "total_mw": 419.408,
          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit delta 2",
          "short_name": "CdhD",
          "sequence": "MAKKMKLSDITNMFAGMDVEALEGVTIEGDIEIDLGGLGGGFDPMLAAALGQESAILAQH\r\nFARLAGMFGYPVGIGAPAAPAVSPALAAPKLKDLIPAKFDVANIAEWATEIQEVPIGNTS\r\nADGGSRGKRVMLGGEKALPFYFDAPMPNRNQVTIDVFDMRIGLAKAVKENYDEVMDSPGE\r\nWAKKNVEKFNADMITIHLISTDPLIKDTPAKEAAKTVEEVLQAVDVPIAIGGSGNPQKDP\r\nEVLARAAEVSEGERCLLASASLNLDYAAIAEAALKYDHDVLSWTQLDMNAQKELNRKLMK\r\nQCNVPRDRIIMDPTTAALGYGLDYAYTNMERIRLAALMGDDELTFPMSSGTTNAWGARES\r\nWMVSSPLKEDSDWGPREYRGPIWEIVTGLSLAIAGNDLFMMMHPTSVAVLKQITQTLFGM\r\nIDTEQVDVANWIGAEV",
          "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)",
          "uniprot_code": "Q8TJC2",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MAKKMKLSDITNMFAGMDVEALEGVTIEGDIEIDLGGLGGGFDPMLAAALGQESAILAQH\nFARLAGMFGYPVGIGAPAAPAVSPALAAPKLKDLIPAKFDVANIAEWATEIQEVPIGNTS\nADGGSRGKRVMLGGEKALPFYFDAPMPNRNQVTIDVFDMRIGLAKAVKENYDEVMDSPGE\nWAKKNVEKFNADMITIHLISTDPLIKDTPAKEAAKTVEEVLQAVDVPIAIGGSGNPQKDP\nEVLARAAEVSEGERCLLASASLNLDYAAIAEAALKYDHDVLSWTQLDMNAQKELNRKLMK\nQCNVPRDRIIMDPTTAALGYGLDYAYTNMERIRLAALMGDDELTFPMSSGTTNAWGARES\nWMVSSPLKEDSDWGPREYRGPIWEIVTGLSLAIAGNDLFMMMHPTSVAVLKQITQTLFGM\nIDTEQVDVANWIGAEV",
          "mw": 47.082,
          "total_mw": 376.656,
          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit gamma",
          "short_name": "CdhE",
          "sequence": ">Q8TH44\r\nMKINSPLEAYKYLPQTNCGECGEATCMAFASKLIDRSGKTSDCPPLIKEKKFAKKLAELD\r\nRLLAPEIRQVTIGVGEKAVNIGGDDVLYRHKLTFFNKTKMFFDVADNMDEAALVERVNSI\r\nANFRKFYVGRNLLLDGVAIRAVSNDPAKFAAAVKKVAEAGLPMIFCSFNPAVLKAGLEAA\r\nKDLKPLLYAANKDNWKEVGELAIEYKVPVVVSAFNDLDALKTLAKTYAEAGIKDIVLDPG\r\nTYPTGKGLKDTFTNFLKIRRAGIMGDTEIAYPIMALPFTAWMAGIADPVSASYWETVMAS\r\nVFTIRYGDIMILHSLEPYATLPEVHLAETIYTDPRTPVSVDGGMYKVGSPTADSPVLFTT\r\nNFALTYYTVESDISSNGIDCWLLAVDTDGIGVEAAVAGGQLTADKVKDAFDKAGFDLKTA\r\nVNHNTVVTPGLAARLQGDLEDKLGANVKVGPMDSGRIPGWMEKNWPPK",
          "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)",
          "uniprot_code": "Q8TH44",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MKINSPLEAYKYLPQTNCGECGEATCMAFASKLIDRSGKTSDCPPLIKEKKFAKKLAELD\nRLLAPEIRQVTIGVGEKAVNIGGDDVLYRHKLTFFNKTKMFFDVADNMDEAALVERVNSI\nANFRKFYVGRNLLLDGVAIRAVSNDPAKFAAAVKKVAEAGLPMIFCSFNPAVLKAGLEAA\nKDLKPLLYAANKDNWKEVGELAIEYKVPVVVSAFNDLDALKTLAKTYAEAGIKDIVLDPG\nTYPTGKGLKDTFTNFLKIRRAGIMGDTEIAYPIMALPFTAWMAGIADPVSASYWETVMAS\nVFTIRYGDIMILHSLEPYATLPEVHLAETIYTDPRTPVSVDGGMYKVGSPTADSPVLFTT\nNFALTYYTVESDISSNGIDCWLLAVDTDGIGVEAAVAGGQLTADKVKDAFDKAGFDLKTA\nVNHNTVVTPGLAARLQGDLEDKLGANVKVGPMDSGRIPGWMEKNWPPK",
          "mw": 50.794,
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          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "50 mM MOPS, 400 mM NaCl, 2 % (w/v) sucrose, 2 mM reduced glutathione (GSH), 2 mM DTT, 2 mM NaNO3, 2 mM ascorbic acid",
        "concentration_unit": null,
        "comment": "Buffer optimized to reduce radiation and oxidative damage",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.2,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Acetyl-CoA decarbonylase/synthase (ACDS), natively purified from Methanosarcina acetivorans",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Marburg, Germany",
            "full_name": "Philipps-Universität Marburg",
            "webpage": ""
          }
        ],
        "contributor_name": "Erik",
        "contributor_surname": "Zimmer",
        "orcid": "https://orcid.org/0009-0005-9366-5283"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-09-07",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 2.0,
    "number_of_frames": 600,
    "wavelength": 0.1,
    "sample_detector_distance": 2.824,
    "concentration_min": null,
    "concentration_max": 5.0,
    "sample_volume": 100.0,
    "flow_rate": 0.25,
    "s_min": 0.056,
    "s_max": 5.58,
    "total_exposure_time": null,
    "seccolumn": 10
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  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.1.1",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Each of the Acetyl-CoA decarbonylase/synthase five subunits (alpha, beta, gamma, delta, epsilon) occurs eight times in the complex, so the total stoichiometry is CdhA8B8C8D8E8.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 2181.63,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 556.5,
  "pddf_i0_error": null,
  "guinier_i0": 563.33,
  "guinier_i0_error": null,
  "pddf_rg": 10.59,
  "pddf_rg_error": null,
  "guinier_rg": 10.85,
  "guinier_rg_error": 0.041,
  "pddf_dmax": 31.44,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 12,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASD2C2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-07-29T16:40:20.923692+02:00",
  "bragg_peak": []
}

{
  "code": "SASD2C2",
  "status": "success",
  "started_at": "2026-08-11T13:35:52.746022+00:00",
  "finished_at": "2026-08-11T13:36:00.701587+00:00",
  "source_last_modified": "2026-07-29T16:40:20.923692+02:00",
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