SASDA28

anti-TG2 antibody (679 14 E06)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-06-21T07:56:39.492710+02:00

Structural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease --- SAXS profile of the autoantibody

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · anti-TG2 antibody (679 14 E06)

浓度2.0 – 16.0 mg/ml缓冲液 / pH20 mM Tris 150mM NaCl 1mM EDTA / 7.2
Experimental temperature10.0 设备 / 束线PETRA III / EMBL P12
波长0.12 nm曝光0.05 s × 20

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
anti-TG2 antibody (679 14 E06)
查看序列
EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
protein—–—monomer分子数 147.639 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11431[3]0.201727–3.96928 1/nm含误差列缺失 00sasbdb/entries/28/sasda28/source/SASDA28.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.10.5nm
i0Guinier1158.96.2
i0P(r)233.1
mwExperimental47.6kDa
mwGuinier I(0)47.6kDa
mwPorod47.5kDa
porod_volumePorod58.0nm³
rgGuinier2.540.02nm
rgP(r)2.58nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 150mM NaCl 1mM EDTA缓冲液浓度20.0 mM
pH7.2添加剂150mM NaCl, 1mM EDTA
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-01-17储存 / 测量温度10.0 / 10.0
曝光时间0.05帧数20
波长0.12样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.202 – 3.969样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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summarysasbdb/entries/28/sasda28/source/summary.jsondownloaded6900178049cab69c1b65323ed29c062fe3722d1d6c41b4592cc5bbc94055d959bfb9Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDA28
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.
project.publication.titleStructural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.
project.publication.author_listChen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M115.669895
project.publication.pmid26160175
project.publication.published_date2015 Aug 28
project.statusreleased
project.submitted_date2015-04-14
project.released_date2015-08-28
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameanti-TG2 antibody (679 14 E06)
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceEVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRY SPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSS ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPS RFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIK RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDS KDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw47.639
experiment.sample.molecule[0].total_mw47.639
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 150mM NaCl 1mM EDTA
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150mM NaCl, 1mM EDTA
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameanti-TG2 antibody (679 14 E06)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMelissa
experiment.contributor[0].contributor_surnameGraewert
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2015-01-17
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_min2.0
experiment.concentration_max16.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.202
experiment.s_max3.969
experiment.total_exposure_time1.0
experiment.seccolumnnull
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pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionStructural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease --- SAXS profile of the autoantibody
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw47.6
experimental_mw_errornull
guinier_i0_mw47.6
guinier_i0_mw_errornull
porod_mw47.5
porod_mw_errornull
pddf_i0233.1
pddf_i0_errornull
guinier_i01158.9
guinier_i0_error6.2
pddf_rg2.58
pddf_rg_errornull
guinier_rg2.54
guinier_rg_error0.02
pddf_dmax8.1
pddf_dmax_error0.5
porod_volume58.0
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estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last119
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
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symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDA28
statussuccess
started_at2026-08-11T15:43:48.819373+00:00
finished_at2026-08-11T15:43:58.485516+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDA28",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.",
    "publication": {
      "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.",
      "author_list": "Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M115.669895",
      "pmid": "26160175",
      "published_date": "2015 Aug 28"
    },
    "status": "released",
    "submitted_date": "2015-04-14",
    "released_date": "2015-08-28"
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查看完整 manifest.json 原文
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