SASDA28

anti-TG2 antibody (679 14 E06)

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-06-21T07:56:39.492710+02:00

Structural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease --- SAXS profile of the autoantibody

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · anti-TG2 antibody (679 14 E06)

Concentration2.0 – 16.0 mg/mlBuffer / pH20 mM Tris 150mM NaCl 1mM EDTA / 7.2
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.12 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
anti-TG2 antibody (679 14 E06)
View sequence
EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
protein———–—monomerMolecule count 147.639 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11431[3]0.201727–3.96928 1/nmHas uncertainty columnMissing 00sasbdb/entries/28/sasda28/source/SASDA28.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.10.5nm
i0Guinier1158.96.2—
i0P(r)233.1——
mwExperimental47.6—kDa
mwGuinier I(0)47.6—kDa
mwPorod47.5—kDa
porod_volumePorod58.0—nm³
rgGuinier2.540.02nm
rgP(r)2.58—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris 150mM NaCl 1mM EDTABuffer concentration20.0 mM
pH7.2Additives150mM NaCl, 1mM EDTA
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-01-17Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength0.12Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.202 – 3.969Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDA28
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.
project.publication.titleStructural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.
project.publication.author_listChen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M115.669895
project.publication.pmid26160175
project.publication.published_date2015 Aug 28
project.statusreleased
project.submitted_date2015-04-14
project.released_date2015-08-28
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameanti-TG2 antibody (679 14 E06)
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceEVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRY SPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSS ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPS RFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIK RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDS KDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw47.639
experiment.sample.molecule[0].total_mw47.639
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 150mM NaCl 1mM EDTA
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150mM NaCl, 1mM EDTA
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameanti-TG2 antibody (679 14 E06)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMelissa
experiment.contributor[0].contributor_surnameGraewert
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2015-01-17
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
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descriptionStructural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease --- SAXS profile of the autoantibody
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw47.6
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guinier_i0_mw47.6
guinier_i0_mw_errornull
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pddf_i0233.1
pddf_i0_errornull
guinier_i01158.9
guinier_i0_error6.2
pddf_rg2.58
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guinier_rg2.54
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pddf_dmax8.1
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porod_volume58.0
porod_volume_errornull
estimated_volumenull
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guinier_point_first1
guinier_point_last119
pddf_point_firstnull
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i0_calibration_standard_datanull
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symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDA28
statussuccess
started_at2026-08-11T15:43:48.819373+00:00
finished_at2026-08-11T15:43:58.485516+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDA28",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.",
    "publication": {
      "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.",
      "author_list": "Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M115.669895",
      "pmid": "26160175",
      "published_date": "2015 Aug 28"
    },
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    "released_date": "2015-08-28"
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View the complete manifest.json source
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