SASDAC6

PsrP functional binding region

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2020-08-28T15:28:14.586213+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · PsrP functional binding region

Concentration— – 2.9 mg/mlBuffer / pHPBS 5 % Glycerol / 7.4
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.09919 nmExposure1.0 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein
View sequence
HHHHHHSGNTIVNGAPAINASLNIAKSETKVYTGEGVDSVYRVPIYYKLKVTNDGSKLTFTYTVTYVNPKTNDLGNISSMRPGYSIYNSGTSTQTMLTLGSDLGKPSGVKNYITDKNGRQVLSYNTSTMTTQGSGYTWGNGAQMNGFFAKKGYGLTSSWTVPITGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTTSQSLSQ
proteinStreptococcus pneumoniaeA0A0H2URK1187–385monomerMolecule count 122.12 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11003[3]0.0716192–4.42086 1/nmHas uncertainty columnMissing 00sasbdb/entries/c6/sasdac6/source/SASDAC6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.8—nm
i0Guinier18.511——
i0P(r)18.64——
mwExperimental18.0—kDa
mwGuinier I(0)17.0—kDa
mwPorod23.6—kDa
porod_volumePorod37.7—nm³
rgGuinier2.039—nm
rgP(r)2.11—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer namePBS 5 % GlycerolBuffer concentration—
pH7.4AdditivesGlycerol 5.000 %
Buffer descriptionPhosphate buffered saline
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-02-27Storage / measurement temperature20.0 / 20.0
Exposure time1.0Frames10
Wavelength0.09919Sample-to-detector distance2.43
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.072 – 4.421Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/c6/sasdac6/source/SASDAC6.datdownloaded4527007975d0c68aba4e94d887655f6d25e8ff392d29a4c70d55dfa5d3dc3851a9d04DownloadView source fileSource site
full_entry_zipsasbdb/entries/c6/sasdac6/source/SASDAC6.zipdownloaded913478f7fa460ff70b6631901103ad3ca817c55c96bc73c32b7054b94743ca7c25355DownloadView source fileSource site
pddfsasbdb/entries/c6/sasdac6/source/SASDAC6.outdownloaded3600213037836c296bd36ccac20bfaf14b831c2dc003eee73ff90ef8a11d19a1d8c07DownloadView source fileSource site
sascifsasbdb/entries/c6/sasdac6/source/SASDAC6.sascifdownloaded4074486dc576e77964e1428c0c555698d9c9c8a822f6d4db0605afcf04d2f592e0f7b8DownloadView source fileSource site
summarysasbdb/entries/c6/sasdac6/source/summary.jsondownloaded12338fda91a08a277a3a0cf2d2976ce1055335e705017e9322876427c47c370c052f6DownloadView source fileSource site
curve:source record
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full_entry_zip:source record and ZIP contents (8 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:169 field values
Field pathRaw value
codeSASDAC6
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleThe BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.
project.publication.titleThe BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.
project.publication.author_listSchulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
project.publication.journalSci Rep
project.publication.doi10.1038/srep32371
project.publication.pmid27582320
project.publication.published_date2016 Sep 1
project.statusreleased
project.submitted_date2015-01-15
project.released_date2016-09-08
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAC6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDAC6.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAC6_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAC6_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAC6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAC6_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDAC6.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFunctional binding region (187-385) of the pneumococcal serine-rich repeat protein
experiment.sample.molecule[0].short_namePsrP BR(187-385)
experiment.sample.molecule[0].sequenceHHHHHHSGNTIVNGAPAINASLNIAKSETKVYTGEGVDSVYRVPIYYKLKVTNDGSKLTFTYTVTYVNPKTNDLGNISSM RPGYSIYNSGTSTQTMLTLGSDLGKPSGVKNYITDKNGRQVLSYNTSTMTTQGSGYTWGNGAQMNGFFAKKGYGLTSSWTVPITGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTTSQSLSQ
experiment.sample.molecule[0].organismStreptococcus pneumoniae
experiment.sample.molecule[0].uniprot_codeA0A0H2URK1
experiment.sample.molecule[0].uniprot_range_first187
experiment.sample.molecule[0].uniprot_range_last385
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTETVEDKVSHSITGLDILKGIVAAGAVISGTVATQTKVFTNESAVLEKTVEKTDALATN DTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESASTSASTSISAS STVVGSQTAAATEATAKKVEEDRKKPASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASI KNAAVFSGNTIVNGAPAINASLNIAKSETKVYTGEGVDSVYRVPIYYKLKVTNDGSKLTF TYTVTYVNPKTNDLGNISSMRPGYSIYNSGTSTQTMLTLGSDLGKPSGVKNYITDKNGRQ VLSYNTSTMTTQGSGYTWGNGAQMNGFFAKKGYGLTSSWTVPITGTDTSFTFTPYAARTD RIGINYFNGGGKVVESSTTSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTS ASASASTSASVSASTSASASASTSASASASTSASESASTSASASASTSASASASTSASAS ASTSASESASTSASASASTSASESASTSASASASTSASASASTSASGSASTSTSASASTS ASASASTSASASASISASESASTSASESASTSTSASASTSASESASTSASASASTSASAS ASTSASASASTSASASTSASESASTSASASASTSASASASTSASASASTSASASASTSAS VSASTSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASISAS ESASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASASAS TSASASASTSASASASTSASASASTSASESASTSASASASTSASESASTSASASASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTS ASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASAS ASISASESASTSASASASTSASVSASTSASASASTSASESASTSASASASTSASESASTS ASASASTSASASASISASESASTSASASASTSASASASTSASASASTSASESASTSTSAS ASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASTSASESASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASESASTSASASASTSASASASTSASASASTSASASASTSASVSASTSASESASTSAS ASASTSASASASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASAS TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSAS ASASTSASASASTSASASASISASESASTSASASASTSASASASTSASVSASTSASASAS TSASASASISASESASTSASASASTSASASASTSASASASTSASASASISASESASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASESASTSASASASTSASASASTSASASASTSASVSASTSASESASTSAS ASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASESASTSASASAS TSASASASTSASASASTSASASASASTSASASASTSASASASTSASASASISASESASTS ASESASTSTSASASTSASESASTSASASASTSASASASTSASASASTSASASTSASESAS TSASASASTSASASASTSASASASTSASASASTSASASASTSASVSASTSASASASTSAS ASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESAS TSASASASTSASASASTSASASASTSASASASTSASASASISASESASTSASASASTSAS ASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASASTSAS ASASTSASASASTSASASASTSASASTSASESASTSASASASTSASASASTSASASASTS ASESASTSASASASTSASASASTSASASASTSASASASTSASASASISASESASTSASAS ASTSASVSASTSASASASTSASESASTSASASASTSASESASTSASASASTSASASASIS ASESASTSASASASTSASASASTSASASASTSASESASTSTSASASTSASESASTSASAS ASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTSASASASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASESASTSASASASTSASASASTSASASASTSASVSASTSASESASTSAS ASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASESASTSASASAS TSASASASTSASASASTSASASASASTSASASASTSASASASTSASASASISASESASTS ASASASASTSASASASTSASASASTSASASASISASESASTSASESASTSTSASASTSAS ESASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTSASASASTS ASASASTSASASASTSASASASTSASVSASTSASASASTSASASASTSASESASTSASAS TSASESASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASTSAS ESASTSASASASTSASASASTSASASASTSASESASTSASASASTSASESASTSASASAS TSASASASTSASGSASTSTSASASTSASASASTSASASASISASESASTSASESASTSTS ASASTSASESASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTS ASASASTSASASASTSASASASTSASVSASTSASASASTSASASASTSASESASTSASAS ASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASTS ASASASTSASASASTSASASASISASESASTSASASASTSASASASTSASASASTSASES ASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTS ASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASAS ASTSASASTSASESASTSASASASTSASASASTSASASASTSASESASTSASASASTSAS ASASTSASASASTSASASASTSASASASISASESASTSASASASTSASVSASTSASASAS TSASESASTSASASASTSASESASTSASASASTSASASASISASESASTSASASASTSAS ASASTSASASASTSASESASTSTSASASTSASESASTSASASASTSASASASTSASASAS TSASASASTSASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASAS ASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTS ASASASTSASVSASTSASESASTSASASASTSASASASTSASESASTSASASASTSASES ASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTS ASASASTSASASASTSASASASTSASASASTSASASASTSASASASISASESASTSASAS ASTSASASASTSASVSASTSASASASTSASASASISASESASTSASASASTSASASASTS ASASASTSASASASISASESASTSASASASTSASASASTSASASASTSASASASTSASAS ASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASIS ASESASTSASASASTSASASASTSASASASTSASESASTSTSASASTSASESASTSASAS ASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTSASASASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASAS TSASASASTSASASASTSASASASTSASVSASTSASESASTSASASASTSASASASTSAS ESASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSAS ASASISASESASTSASASASTSASASASTSASVSASTSASASASTSASASASISASESAS TSASASASTSASASASTSASASASTSASASASISASESASTSASASASTSASASASTSAS ASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAS TSASASASTSASASASTSASASASTSASASASTSVSNSANHSNSQVGNTSGSTGKSQKEL PNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE
experiment.sample.molecule[0].mw22.12
experiment.sample.molecule[0].total_mw22.12
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.namePBS 5 % Glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentPhosphate buffered saline
experiment.sample.buffer.additiveGlycerol 5.000 %
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePsrP functional binding region
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAl
experiment.contributor[0].contributor_surnameKikhney
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-1321-3956
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-02-27
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames10
experiment.wavelength0.09919
experiment.sample_detector_distance2.43
experiment.concentration_minnull
experiment.concentration_max2.9
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.072
experiment.s_max4.421
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDAC6_fit1_model1_img.png
fits[0].models[0].softwareEOM/RANCH
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelmix
fits[0].models[0].software_version1.3
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDAC6_fit1_model1.pdb
fits[0].models[0].model_mw22.35
fits[0].models[0].bead_radius1.9
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/GA001_9pahFzE.log
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user4
fits[0].models[1].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDAC6_fit1_model2_img.png
fits[0].models[1].softwareEOM/RANCH
fits[0].models[1].pdb_link[]
fits[0].models[1].model_titlenull
fits[0].models[1].type_of_modelmix
fits[0].models[1].software_version1.4
fits[0].models[1].model_datahttps://www.sasbdb.org/media/pdb_file/SASDAC6_fit1_model2.pdb
fits[0].models[1].model_mw22.35
fits[0].models[1].bead_radius1.9
fits[0].models[1].lognull
fits[0].models[1].symmetry
fits[0].models[1].comment
fits[0].models[1].user4
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAC6_fit1_fixed_fit_img.png
fits[0].softwarenull
fits[0].chi_square_value0.926
fits[0].p_value0.0
fits[0].fit_residual_plotfit4columns470_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDAC6_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGNOM
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw18.0
experimental_mw_errornull
guinier_i0_mw17.0
guinier_i0_mw_errornull
porod_mw23.6
porod_mw_errornull
pddf_i018.64
pddf_i0_errornull
guinier_i018.511
guinier_i0_errornull
pddf_rg2.11
pddf_rg_errornull
guinier_rg2.039
guinier_rg_errornull
pddf_dmax7.8
pddf_dmax_errornull
porod_volume37.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first40
guinier_point_last108
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAC6_datloglog_img.png
symmetrynull
last_modified2020-08-28T15:28:14.586213+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAC6
statussuccess
started_at2026-08-11T16:26:06.479102+00:00
finished_at2026-08-11T16:26:15.469696+00:00
source_last_modified2020-08-28T15:28:14.586213+02:00
files[0].statusdownloaded
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View the complete summary.json source
{
  "code": "SASDAC6",
  "status": "Published",
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  "angular_unit": "1/nm",
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    "title": "The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.",
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      "title": "The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.",
      "author_list": "Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A",
      "journal": "Sci Rep",
      "doi": "10.1038/srep32371",
      "pmid": "27582320",
      "published_date": "2016 Sep 1"
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View the complete manifest.json source
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