SASDAJ8

Light state solution structure of Aureochrome1a- A´α-LOV-Jα

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2019-12-06T13:07:15.624532+01:00

Solution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar concentration. The buffer-only scattering was subtracted and the data sets were merged to generate representative curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Light state solution structure of Aureochrome1a- A´α-LOV-Jα

Concentration0.75 – 15.0 Buffer / pH10 mM Tris 300 mM NaCl / 8.0
Experimental temperature— Facility / beamlineESRF / BM29
Wavelength0.93 nmExposure20.0 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Aureochrome1a-A´α-LOV-Jα
View sequence
MGSSHHHHHHSSGLVPRGSHMDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
proteinPhaeodactylum tricornutum——–—dimerMolecule count 217.973 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11043[3]0.0336923–4.97517 1/nmHas uncertainty columnMissing 00sasbdb/entries/j8/sasdaj8/source/SASDAJ8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.0—nm
i0Guinier62.75——
i0P(r)63.8——
mwExperimental63.0—kDa
porod_volumePorod87.66—nm³
rgGuinier3.34—nm
rgP(r)3.51—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM Tris 300 mM NaClBuffer concentration10.0 mM
pH8.0Additives300 mM NaCl
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-03-16Storage / measurement temperature4.0 / —
Exposure time20.0Frames10
Wavelength0.93Sample-to-detector distance2.43
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.034 – 4.975Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
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codeSASDAJ8
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.
project.publication.titleStructure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.
project.publication.author_listBanerjee A, Herman E, Kottke T, Essen LO
project.publication.journalStructure
project.publication.doi10.1016/j.str.2015.10.022
project.publication.pmid26688213
project.publication.published_date2016 Jan 5
project.statusreleased
project.submitted_date2015-06-18
project.released_date2016-01-05
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experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
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experiment.sample.molecule[0].short_nameAureochrome1a-A
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
experiment.sample.molecule[0].organismPhaeodactylum tricornutum
experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.buffer.name10 mM Tris 300 mM NaCl
experiment.sample.buffer.concentration_unitmM
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experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
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experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMarburg, Germany
experiment.contributor[0].affiliation[0].full_namePhilipps-Universität Marburg
experiment.contributor[0].affiliation[0].webpage
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descriptionSolution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar concentration. The buffer-only scattering was subtracted and the data sets were merged to generate representative curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
experiment_descriptionnull
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last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
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View the complete manifest.json source
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