SASDAK6

Glucose Isomerase

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2023-05-25T11:21:52.654103+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Glucose Isomerase

浓度1.2 – 12.83 mg/ml缓冲液 / pHPBS, 50% Glycerol, 0.076 M NaCl / 7.4
实验温度— 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光15.0 s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Xylose isomerase
查看序列
>SP|P24300|XYLA_STRRUXYLOSEISOMERASEOS=STREPTOMYCESRUBIGINOSUSGN=XYLAPE=1SV=4MNYQPTPEDRFTFGLWTVGWQGRDPFGDATRRALDPVESVRRLAELGAHGVTFHDDDLIPFGSSDSEREEHVKRFRQALDDTGMKVPMATTNLFTHPVFKDGGFTANDRDVRRYALRKTIRNIDLAVELGAETYVAWGGREGAESGGAKDVRDALDRMKEAFDLLGEYVTSQGYDIRFAIEPKPNEPRGDILLPTVGHALAFIERLERPELYGVNPEVGHEQMAGLNFPHGIAQALWAGKLFHIDLNGQNGIKYDQDLRFGAGDLRAAFWLVDLLESAGYSGPRHFDFKPPRTEDFDGVWASAAGCMRNYLILKERAAAFRADPEVQEALRASRLDELARPTAADGLQALLDDRSAFEEFDVDAAAARGMAFERLDQLAMDHLLGARG
proteinStreptomyces rubiginosusP24300—–—tetramer分子数 443.23 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12169[3]0.0871252–6.01727 1/nm含误差列缺失 00sasbdb/entries/k6/sasdak6/source/SASDAK6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.74—nm
i0Guinier165.24——
i0P(r)162.5——
mwExperimental184.02—kDa
mwGuinier I(0)184.02—kDa
mwPorod141.1—kDa
porod_volumePorod293.1—nm³
rgGuinier3.4—nm
rgP(r)3.288—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称PBS, 50% Glycerol, 0.076 M NaCl缓冲液浓度—
pH7.4添加剂Glycerol 50.000 % , NaCl 0.076 M
缓冲液说明—
纯度测定方法—消光系数—
吸收值11.12散射对比度—
比体积 / 干体积— / 209混合物 / 氘代— / —

采集条件与仪器

测量日期2012-04-24储存 / 测量温度— / —
曝光时间15.0帧数8
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q 范围0.087 – 6.017样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/k6/sasdak6/source/SASDAK6.datdownloaded9805378c0e55dbb1f18af32f1fdef2870db7f46479aa0568d6e2761f4cff443139f9a下载查看原文件源站
full_entry_zipsasbdb/entries/k6/sasdak6/source/SASDAK6.zipdownloaded3806481c58440a800c88aec7e7815227d1e09d4f1a9b2919a62acb5585fba09ba323cc下载查看原文件源站
pddfsasbdb/entries/k6/sasdak6/source/SASDAK6.outdownloaded1029485ce93e68d0a3954d4fe7ea98ac64c610354ad37bd0d60d7ac0db93706f7983c1下载查看原文件源站
sascifsasbdb/entries/k6/sasdak6/source/SASDAK6.sascifdownloaded20780284084752d36c7eb67af4f90de241e81b19b7303950640d18d0fe202ee6c340407下载查看原文件源站
summarysasbdb/entries/k6/sasdak6/source/summary.jsondownloaded8126efd4361fbbaa36e7efa9a3a8a9914309f04c5f98e444fa6a0bd8f233fe30adcd下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:177 个字段值
字段路径原始值
codeSASDAK6
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleStandard proteins
project.publicationnull
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameXylose isomerase
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence>sp|P24300|XYLA_STRRU Xylose isomerase OS=Streptomyces rubiginosus GN=xylA PE=1 SV=4 MNYQPTPEDRFTFGLWTVGWQGRDPFGDATRRALDPVESVRRLAELGAHGVTFHDDDLIP FGSSDSEREEHVKRFRQALDDTGMKVPMATTNLFTHPVFKDGGFTANDRDVRRYALRKTI RNIDLAVELGAETYVAWGGREGAESGGAKDVRDALDRMKEAFDLLGEYVTSQGYDIRFAI EPKPNEPRGDILLPTVGHALAFIERLERPELYGVNPEVGHEQMAGLNFPHGIAQALWAGK LFHIDLNGQNGIKYDQDLRFGAGDLRAAFWLVDLLESAGYSGPRHFDFKPPRTEDFDGVW ASAAGCMRNYLILKERAAAFRADPEVQEALRASRLDELARPTAADGLQALLDDRSAFEEF DVDAAAARGMAFERLDQLAMDHLLGARG
experiment.sample.molecule[0].organismStreptomyces rubiginosus
experiment.sample.molecule[0].uniprot_codeP24300
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw43.23
experiment.sample.molecule[0].total_mw172.3
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.namePBS, 50% Glycerol, 0.076 M NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveGlycerol 50.000 % , NaCl 0.076 M
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameGlucose Isomerase
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_vol209
experiment.sample.absorbption11.12
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameErica
experiment.contributor[0].contributor_surnameValentini
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2012-04-24
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_min1.2
experiment.concentration_max12.83
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.087
experiment.s_max6.017
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
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fits[0].p_value0.0
fits[0].fit_residual_plotSASDAK6_fit1_fitresiduals_img.png
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fits[1].chi_square_value2.047761
fits[1].p_value0.0
fits[1].fit_residual_plotSASDAK6_fit2_fitresiduals_img.png
fits[1].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDAK6_fit2.fit
fits[1].fit_lognull
fits[1].software_versionnull
fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versiondatgnom
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw184.02
experimental_mw_errornull
guinier_i0_mw184.02
guinier_i0_mw_errornull
porod_mw141.1
porod_mw_errornull
pddf_i0162.5
pddf_i0_errornull
guinier_i0165.24
guinier_i0_errornull
pddf_rg3.288
pddf_rg_errornull
guinier_rg3.4
guinier_rg_errornull
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pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first30
guinier_point_last109
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAK6_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAK6
statussuccess
started_at2026-08-11T15:11:13.430554+00:00
finished_at2026-08-11T15:11:31.250193+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Standard proteins",
    "publication": null,
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
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查看完整 manifest.json 原文
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