SASDAL6

Wild-type chalcone isomerase, ligand-free

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Wild-type chalcone isomerase, ligand-free

Concentration1.38 – 15.3 Buffer / pH50 mM sodium phosphate / 6.8
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Bacterial chalcone isomerase
View sequence
>TR|V9P0A9|V9P0A9_EUBRACHALCONEISOMERASEOS=EUBACTERIUMRAMULUSGN=TAIPE=1SV=1MADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQGDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGDAGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGEKWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALEKPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR
proteinEubacterium ramulusV9P0A9—–—hexamerMolecule count 632.4 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11667[3]0.0754087–4.45777 1/nmHas uncertainty columnMissing 01sasbdb/entries/l6/sasdal6/source/SASDAL6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.00.5nm
i0Guinier32346.5—arbitrary
i0P(r)25450.0—arbitrary
mwExperimental190.0—kDa
mwGuinier I(0)190.0—kDa
mwPorod190.0—kDa
porod_volumePorod320.0—nm³
rgGuinier3.97—nm
rgP(r)3.7—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM sodium phosphateBuffer concentration—
pH6.8Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast3.047
Specific volume / dry volume— / —Mixture / deuterationFalse / —

Acquisition Conditions and Instrument

Measurement date2013-09-23Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength0.124Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.075 – 4.458Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDAL6
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleStructure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.
project.publication.titleStructure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.
project.publication.author_listThomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
project.publication.journalActa Crystallogr D Biol Crystallogr
project.publication.doi10.1107/S1399004715001935
project.publication.pmid25849401
project.publication.published_date2015 Apr
project.statusreleased
project.submitted_date2015-07-21
project.released_date2017-11-06
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBacterial chalcone isomerase
experiment.sample.molecule[0].short_nameCHI
experiment.sample.molecule[0].sequence>tr|V9P0A9|V9P0A9_EUBRA Chalcone isomerase OS=Eubacterium ramulus GN=tai PE=1 SV=1 MADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQ GDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGD AGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGE KWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALE KPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR
experiment.sample.molecule[0].organismEubacterium ramulus
experiment.sample.molecule[0].uniprot_codeV9P0A9
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationhexamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQ GDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGD AGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGE KWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALE KPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR
experiment.sample.molecule[0].mw32.4
experiment.sample.molecule[0].total_mw194.4
experiment.sample.molecule[0].number_molecules6
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM sodium phosphate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameWild-type chalcone isomerase, ligand-free
experiment.sample.ext_coefficientnull
experiment.sample.contrast3.047
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixtureFalse
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAnne
experiment.contributor[0].contributor_surnameTuukkanen
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-09-23
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance3.1
experiment.concentration_min1.38
experiment.concentration_max15.3
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.075
experiment.s_max4.458
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw190.0
experimental_mw_errornull
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guinier_i0_mw_errornull
porod_mw190.0
porod_mw_errornull
pddf_i025450.0
pddf_i0_errornull
guinier_i032346.5
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pddf_rg_errornull
guinier_rg3.97
guinier_rg_errornull
pddf_dmax13.0
pddf_dmax_error0.5
porod_volume320.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last73
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAL6_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAL6
statussuccess
started_at2026-08-11T15:11:43.772734+00:00
finished_at2026-08-11T15:11:52.763729+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDAL6",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.",
    "publication": {
      "title": "Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.",
      "author_list": "Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W",
      "journal": "Acta Crystallogr D Biol Crystallogr",
      "doi": "10.1107/S1399004715001935",
      "pmid": "25849401",
      "published_date": "2015 Apr"
    },
    "status": "released",
    "submitted_date": "2015-07-21",
    "released_date": "2017-11-06"
  },
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          "long_name": "Bacterial chalcone isomerase",
          "short_name": "CHI",
          "sequence": ">tr|V9P0A9|V9P0A9_EUBRA Chalcone isomerase OS=Eubacterium ramulus GN=tai PE=1 SV=1\r\nMADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQ\r\nGDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGD\r\nAGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGE\r\nKWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALE\r\nKPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR",
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View the complete manifest.json source
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