SASDAP7

PBX1-PREB1 complex

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-06-21T07:56:39.492710+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · PBX1-PREB1 complex

浓度1.5 – 2.8 mg/ml缓冲液 / pH20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT / 7.4
实验温度设备 / 束线PETRA III / EMBL P12
波长0.12 nm曝光0.05 s × 50

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Pre-B-cell leukemia transcription factor 1
查看序列
MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAAK
proteinHomo sapiensP404241–308monomer分子数 134.455 kDa
Homeobox protein PKNOX1
查看序列
MMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYRHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTEMNSETLLSGEPGSPYSPVQSQQIQSAITGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRF
proteinHomo sapiensP553471–344monomer分子数 138.339 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11682[3]0.027543–4.45043 1/nm含误差列缺失 017sasbdb/entries/p7/sasdap7/source/SASDAP7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)19.0nm
i0Guinier1580.53
i0P(r)1580.0
mwExperimental77.0kDa
mwGuinier I(0)77.0kDa
mwPorod84.0kDa
porod_volumePorod143.0nm³
rgGuinier5.846nm
rgP(r)5.7nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT缓冲液浓度20.0 mM
pH7.4添加剂150 mM NaCl, 5% glycerol, 1 mM DTT
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-12-06储存 / 测量温度— / —
曝光时间0.05帧数50
波长0.12样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.028 – 4.45样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/p7/sasdap7/source/SASDAP7.datdownloaded75961a37a3714d518477e94e05b8b5e174aa3f0fcb9c4f3efdf463b342e2df2eb02a9下载查看原文件源站
full_entry_zipsasbdb/entries/p7/sasdap7/source/SASDAP7.zipdownloaded43306916b3ce7cec4efb8de275fb3332018fe50fcb5bb986298663aae3d1a4eb60fd9下载查看原文件源站
pddfsasbdb/entries/p7/sasdap7/source/SASDAP7.outdownloaded37553915170d48430bcf3ea8b97f81b84fea6cccfc4b444b16665b3b4012fc7cde4a3下载查看原文件源站
sascifsasbdb/entries/p7/sasdap7/source/SASDAP7.sascifdownloaded20244768801e10d74e38abc6ae2d20de311a6eacc37dfe251a08726744fbabcf234e08下载查看原文件源站
summarysasbdb/entries/p7/sasdap7/source/summary.jsondownloaded8552af7c8771f2623e236fa41978eb3c3a80d96fa492bf69567f186e2b8583c294b6下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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  "zip_member_count": 4,
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:173 个字段值
字段路径原始值
codeSASDAP7
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleThe flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
project.publication.titleThe flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
project.publication.author_listMathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
project.publication.journalFEBS J
project.publication.doi10.1111/febs.13801
project.publication.pmid27390177
project.publication.published_date2016 Aug
project.statusreleased
project.submitted_datenull
project.released_date2016-07-25
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAP7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDAP7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAP7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDAP7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePre-B-cell leukemia transcription factor 1
experiment.sample.molecule[0].short_namePBX1
experiment.sample.molecule[0].sequenceMDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDE AQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGP EKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVM NLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNF NKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQE EANIYAAK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP40424
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last308
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDE AQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGP EKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVM NLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNF NKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQE EANIYAAKTAVTATNVSAHGSQANSPSTPNSAGSSSSFNMSNSGDLFMSVQSLNGDSYQG AQVGANVQSQVDTLRHVISQTGGYSDGLAASQMYSPQGISANGGWQDATTPSSVTSPTEG PGSVHSDTSN
experiment.sample.molecule[0].mw34.455
experiment.sample.molecule[0].total_mw34.455
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameHomeobox protein PKNOX1
experiment.sample.molecule[1].short_namePREP1
experiment.sample.molecule[1].sequenceMMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYR HPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVK AIQVLRIHLLELEKVNELCKDFCSRYIACLKTEMNSETLLSGEPGSPYSPVQSQQIQSAI TGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQ LQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTN LTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRF
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeP55347
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last344
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYR HPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVK AIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLLSGEPGSPYSPVQSQQIQSAI TGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQ LQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTN LTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRFWPDSIASGVAQPPPS ELTMSEGAVVTITTPVNMNVDSLQSLSSDGATLAVQQVMMAGQSEDESVDSTEEDAGALA PAHISGLVLENSDSLQ
experiment.sample.molecule[1].mw38.339
experiment.sample.molecule[1].total_mw38.339
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl, 5% glycerol, 1 mM DTT
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePBX1-PREB1 complex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameErica
experiment.contributor[0].contributor_surnameValentini
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-12-06
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time0.05
experiment.number_of_frames50
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_min1.5
experiment.concentration_max2.8
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.028
experiment.s_max4.45
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDAP7_fit1_model1_img.png
fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_version
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDAP7_fit1_model1.pdb
fits[0].models[0].model_mw104.154
fits[0].models[0].bead_radius4.0
fits[0].models[0].lognull
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user1
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAP7_fit1_fit_img.png
fits[0].softwarenull
fits[0].chi_square_value1.340964
fits[0].p_value7e-06
fits[0].fit_residual_plotSASDAP7_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDAP7_fit1.fir
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw77.0
experimental_mw_errornull
guinier_i0_mw77.0
guinier_i0_mw_errornull
porod_mw84.0
porod_mw_errornull
pddf_i01580.0
pddf_i0_errornull
guinier_i01580.53
guinier_i0_errornull
pddf_rg5.7
pddf_rg_errornull
guinier_rg5.846
guinier_rg_errornull
pddf_dmax19.0
pddf_dmax_errornull
porod_volume143.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first29
guinier_point_last75
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAP7_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAP7
statussuccess
started_at2026-08-11T15:35:27.320178+00:00
finished_at2026-08-11T15:35:39.135556+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAP7",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.",
    "publication": {
      "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.",
      "author_list": "Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C",
      "journal": "FEBS J",
      "doi": "10.1111/febs.13801",
      "pmid": "27390177",
      "published_date": "2016 Aug"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2016-07-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDAP7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAP7.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_dat_img.png",
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查看完整 manifest.json 原文
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