SASDAS7

mouse olfactomedin-1

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00

SAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · mouse olfactomedin-1

Concentration— – 3.55 mg/mlBuffer / pH20 mM HEPES 150 mM NaCl / 7.5
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.93 nmExposure0.056 s × 18

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Noelin
View sequence
MITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESWQVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQYVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQFKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVKTSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVDENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSGGTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW
proteinMus musculusO88998—–—tetramerMolecule count 464.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11037[3]0.02801756–4.493585 1/nmHas uncertainty columnMissing 029sasbdb/entries/s7/sasdas7/source/SASDAS7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)30.0—nm
i0Guinier249.00.3—
i0P(r)250.90.2—
mwExperimental249.0—kDa
mwPorod323.0—kDa
porod_volumePorod616.0—nm³
rgGuinier8.5—nm
rgP(r)8.790.008nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES 150 mM NaClBuffer concentration20.0 mM
pH7.5Additives150 mM NaCl
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-11-06Storage / measurement temperature20.0 / 20.0
Exposure time0.056Frames18
Wavelength0.93Sample-to-detector distance2.43
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.028 – 4.494Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDAS7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleOlfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.
project.publication.titleOlfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.
project.publication.author_listPronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M115.653485
project.publication.pmid25903135
project.publication.published_date2015 Jun 12
project.statusreleased
project.submitted_date2015-03-12
project.released_date2015-04-27
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNoelin
experiment.sample.molecule[0].short_nameOlfactomedin-1
experiment.sample.molecule[0].sequenceMITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESW QVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQ YVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQ FKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVK TSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSG TGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVD ENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeO88998
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSVPLLKIGVVLSTMAMITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESW QVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQ YVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQ FKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVK TSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSG TGQVVYNGSIYFNKFQSHIIIRFDLKTEAILKTRSLDYAGYNNMYHYAWGGHSDIDLMVD ENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAWNNGHQTLYNVTLFHVI RSDEL
experiment.sample.molecule[0].mw64.0
experiment.sample.molecule[0].total_mw256.0
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES 150 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namemouse olfactomedin-1
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressUtrecht, Netherlands
experiment.contributor[0].affiliation[0].full_nameUtrecht University
experiment.contributor[0].affiliation[0].webpagehttp://www.uu.nl/
experiment.contributor[0].contributor_nameMatti
experiment.contributor[0].contributor_surnamePronker
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-11-06
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.056
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experiment.sample_detector_distance2.43
experiment.concentration_minnull
experiment.concentration_max3.55
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.028
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estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionSAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw249.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw323.0
porod_mw_errornull
pddf_i0250.9
pddf_i0_error0.2
guinier_i0249.0
guinier_i0_error0.3
pddf_rg8.79
pddf_rg_error0.008
guinier_rg8.5
guinier_rg_errornull
pddf_dmax30.0
pddf_dmax_errornull
porod_volume616.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
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pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAS7_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAS7
statussuccess
started_at2026-08-11T15:37:35.188528+00:00
finished_at2026-08-11T15:37:42.943401+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDAS7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.",
    "publication": {
      "title": "Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.",
      "author_list": "Pronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M115.653485",
      "pmid": "25903135",
      "published_date": "2015 Jun 12"
    },
    "status": "released",
    "submitted_date": "2015-03-12",
    "released_date": "2015-04-27"
  },
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      "beamline_name": "BM29",
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          "long_name": "Noelin",
          "short_name": "Olfactomedin-1",
          "sequence": "MITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESW QVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQ YVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQ FKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVK TSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSG TGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVD ENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW",
          "organism": "Mus musculus",
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          "molecular_type": "protein",
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          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
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