SASDAS7

mouse olfactomedin-1

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

SAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · mouse olfactomedin-1

浓度— – 3.55 mg/ml缓冲液 / pH20 mM HEPES 150 mM NaCl / 7.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.93 nm曝光0.056 s × 18

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Noelin
查看序列
MITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESWQVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQYVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQFKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVKTSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVDENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSGGTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW
proteinMus musculusO88998—–—tetramer分子数 464.0 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11037[3]0.02801756–4.493585 1/nm含误差列缺失 029sasbdb/entries/s7/sasdas7/source/SASDAS7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)30.0—nm
i0Guinier249.00.3—
i0P(r)250.90.2—
mwExperimental249.0—kDa
mwPorod323.0—kDa
porod_volumePorod616.0—nm³
rgGuinier8.5—nm
rgP(r)8.790.008nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES 150 mM NaCl缓冲液浓度20.0 mM
pH7.5添加剂150 mM NaCl
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-11-06储存 / 测量温度20.0 / 20.0
曝光时间0.056帧数18
波长0.93样品-探测器距离2.43
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.028 – 4.494样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDAS7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleOlfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.
project.publication.titleOlfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.
project.publication.author_listPronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M115.653485
project.publication.pmid25903135
project.publication.published_date2015 Jun 12
project.statusreleased
project.submitted_date2015-03-12
project.released_date2015-04-27
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAS7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDAS7.dat
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNoelin
experiment.sample.molecule[0].short_nameOlfactomedin-1
experiment.sample.molecule[0].sequenceMITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESW QVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQ YVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQ FKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVK TSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSG TGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVD ENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeO88998
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw64.0
experiment.sample.molecule[0].total_mw256.0
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES 150 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namemouse olfactomedin-1
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressUtrecht, Netherlands
experiment.contributor[0].affiliation[0].full_nameUtrecht University
experiment.contributor[0].affiliation[0].webpagehttp://www.uu.nl/
experiment.contributor[0].contributor_nameMatti
experiment.contributor[0].contributor_surnamePronker
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-11-06
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.056
experiment.number_of_frames18
experiment.wavelength0.93
experiment.sample_detector_distance2.43
experiment.concentration_minnull
experiment.concentration_max3.55
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.028
experiment.s_max4.494
experiment.total_exposure_time1.0
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
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fits[0].models[0].type_of_modeldummy
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fits[0].models[0].bead_radius6.3
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fits[0].models[0].comment
fits[0].models[0].user52
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fits[0].softwarenull
fits[0].chi_square_value4.313
fits[0].p_value0.0
fits[0].fit_residual_plotSASDAS7_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionSAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw249.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw323.0
porod_mw_errornull
pddf_i0250.9
pddf_i0_error0.2
guinier_i0249.0
guinier_i0_error0.3
pddf_rg8.79
pddf_rg_error0.008
guinier_rg8.5
guinier_rg_errornull
pddf_dmax30.0
pddf_dmax_errornull
porod_volume616.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAS7_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAS7
statussuccess
started_at2026-08-11T15:37:35.188528+00:00
finished_at2026-08-11T15:37:42.943401+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDAS7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.",
    "publication": {
      "title": "Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.",
      "author_list": "Pronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M115.653485",
      "pmid": "25903135",
      "published_date": "2015 Jun 12"
    },
    "status": "released",
    "submitted_date": "2015-03-12",
    "released_date": "2015-04-27"
  },
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        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
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      "xray_energy": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Noelin",
          "short_name": "Olfactomedin-1",
          "sequence": "MITNWMSQTLPSLVGLNTTRLSAASGGTLDRSTGVLPTNPEESW QVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQ YVEKMENQMKGLETKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQ FKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVK TSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMVDFMNTDNFTSHRLPHPWSG TGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVD ENGLWAVYATNQNAGNIVISKLDPVSLQILQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAW",
          "organism": "Mus musculus",
          "uniprot_code": "O88998",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
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          "molecular_type": "protein",
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}
查看完整 manifest.json 原文
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