2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
dmax
P(r)
12.54
—
nm
i0
Guinier
43.62
—
—
i0
P(r)
42.66
—
—
mw
Experimental
43.5
—
kDa
mw
Porod
60.5
—
kDa
porod_volume
Porod
121.45
—
nm³
rg
Guinier
3.89
—
nm
rg
P(r)
3.872
—
nm
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
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3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
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4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/24/sasdb24/source/SASDB24.dat
downloaded
37338
full_entry_zip
sasbdb/entries/24/sasdb24/source/SASDB24.zip
downloaded
25414
pddf
sasbdb/entries/24/sasdb24/source/SASDB24.out
downloaded
55861
sascif
sasbdb/entries/24/sasdb24/source/SASDB24.sascif
downloaded
82969
summary
sasbdb/entries/24/sasdb24/source/summary.json
downloaded
6363
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5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
X-ray synchrotron radiation scattering data from solutions of light-state PtAUREO1a bZIP-LOV module in 10mM Tris HCl pH 8 300mM NaCl buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of bZIP-LOV module in the light-state was performed at 0.625-2.5mg/mL concentration at 4 degrees centigrade. The SAXS data derived from the concentration series were merged for analysis using the ATSAS package. For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
Related Publication
Title
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
Authors
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
DOI / PMID
10.1093/nar/gkw420 / 27179025
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
X-ray synchrotron radiation scattering data from solutions of light-state PtAUREO1a bZIP-LOV module in 10mM Tris HCl pH 8 300mM NaCl buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of bZIP-LOV module in the light-state was performed at 0.625-2.5mg/mL concentration at 4 degrees centigrade. The SAXS data derived from the concentration series were merged for analysis using the ATSAS package. For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.