SASDB24

Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2019-12-06T13:07:15.624532+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

浓度0.63 – 2.5 mg/ml缓冲液 / pH10 mM Tris 300 mM NaCl / 8.0
实验温度4.0 设备 / 束线ESRF / BM29
波长0.93 nm曝光20.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Aureochrome 1a bZIP-LOV module
查看序列
MGSSHHHHHHSSGLVPRGSHMDRKMSEQQKVERRERNREHAKRSRIRKKFLLESLQQSVSLLKEENEKLKTSIRSHLGEEKADTLIDSANNNKTDVDGLLASSQGIANKVLDDPDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
proteinPhaeodactylum tricornutum145–378dimer分子数 228.655 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1800[3]0.0363783–3.82291 1/nm含误差列缺失 00sasbdb/entries/24/sasdb24/source/SASDB24.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)12.54nm
i0Guinier43.62
i0P(r)42.66
mwExperimental43.5kDa
mwPorod60.5kDa
porod_volumePorod121.45nm³
rgGuinier3.89nm
rgP(r)3.872nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10 mM Tris 300 mM NaCl缓冲液浓度10.0 mM
pH8.0添加剂300 mM NaCl
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-11-06储存 / 测量温度4.0 / 4.0
曝光时间20.0帧数10
波长0.93样品-探测器距离2.43
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 3.823样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDB24
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleAllosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
project.publication.titleAllosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
project.publication.author_listBanerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkw420
project.publication.pmid27179025
project.publication.published_date2016 Jul 8
project.statusreleased
project.submitted_date2016-02-14
project.released_date2016-09-19
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAureochrome 1a bZIP-LOV module
experiment.sample.molecule[0].short_namePtAUREO1a bZIP-LOV
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMDRKMSEQQKVERRERNREHAKRSRIRKKFLLESLQQSVSLLKEENEKLKTSIRSHLGEEKADTLIDSANNNKTDVDGLLASSQGIANKVLDDPDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
experiment.sample.molecule[0].organismPhaeodactylum tricornutum
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first145
experiment.sample.molecule[0].uniprot_range_last378
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw28.655
experiment.sample.molecule[0].total_mw57.309
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10 mM Tris 300 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive300 mM NaCl
experiment.sample.buffer.concentration10.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameAureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMarburg, Germany
experiment.contributor[0].affiliation[0].full_namePhilipps-Universität Marburg
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameAnkan
experiment.contributor[0].contributor_surnameBanerjee
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2014-11-06
experiment.storage_temperature4.0
experiment.cell_temperature4.0
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experiment.number_of_frames10
experiment.wavelength0.93
experiment.sample_detector_distance2.43
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experiment.flow_ratenull
experiment.s_min0.036
experiment.s_max3.823
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of light-state PtAUREO1a bZIP-LOV module in 10mM Tris HCl pH 8 300mM NaCl buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of bZIP-LOV module in the light-state was performed at 0.625-2.5mg/mL concentration at 4 degrees centigrade. The SAXS data derived from the concentration series were merged for analysis using the ATSAS package. For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
tags[]
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guinier_i0_mwnull
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pddf_dmax_errornull
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porod_volume_errornull
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guinier_point_last60
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDB24_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDB24
statussuccess
started_at2026-08-11T14:12:07.248153+00:00
finished_at2026-08-11T14:12:14.818052+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.",
    "publication": {
      "title": "Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.",
      "author_list": "Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T",
      "journal": "Nucleic Acids Res",
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  "pddf_dmax_error": null,
  "porod_volume": 121.45,
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  "guinier_point_first": 8,
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  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDB24_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDB24",
  "status": "success",
  "started_at": "2026-08-11T14:12:07.248153+00:00",
  "finished_at": "2026-08-11T14:12:14.818052+00:00",
  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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}