Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA

{
  "code": "SASDB27",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.",
    "publication": {
      "title": "Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.",
      "author_list": "Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF",
      "journal": "Biosci Rep",
      "doi": "10.1042/BSR20160435",
      "pmid": "27742866",
      "published_date": "2016 Dec"
    },
    "status": "released",
    "submitted_date": "2016-10-10",
    "released_date": "2016-10-16"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDB27.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB27.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB27_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB27.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "MAR 165 CCD",
        "resolution": null
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Proline utilization A",
          "short_name": "PutA",
          "sequence": "MGSSHHHHHHSSGLVPRGSH\r\nMGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSY\r\nLEQLENSDTLPEH\r\nMTDLSALGPKAKFAPEAEVLQALVAQAALPQPQLDRIAARGADLVAR\r\nIRAEAKPSLMEHFLAQYGLSTREGVALMCLAEAMLRVPDTATIDALIEDKIAPSDWGKHL\r\nGTAASSLVNASTWALMLTGKVLDDGAGGIAGTLRGAMRRLGEPVIRAAVGQAMREMGRQF\r\nVLGETIEKALERAEKREAEGYTFSYDMLGEAALTAADAERYRLAYAQAITAIGKAATRGS\r\nIAANPGISIKLSALHPRYEVAQEARVMAELVPVVRDLARAAARAGIALHIDAEEQDRLAL\r\nSLRVMAAVIADPETAGWEGFGAVVQAYGKRAGAAIDALAAMARAAGRRINIRLVKGAYWD\r\nAEMKRAQVEGHPGFPLFTSKTGTDVAYICLAAKLFGLNDCIYPQFATHNAHTVAAVLEMA\r\nAGRPFEFQRLHGMGARLHDIVLRETGGRCRIYAPVGAHRDLLAYLVRRLLENGANSSFVN\r\nQIVNESVPPAEVAACPFAALPTARAPRGLLAPADLFGAGRVNAQGFDLSDPEVLARIEAA\r\nRDVTLPDAAPIVAGPVSGTLRPVRNPATGAVVAQVTEADAATVALALDAAQVWSAPAATR\r\nAAVLCRAADLYEENFGPIFAALAQEAGKTLGDAVSELREAVDFLRYYAAEGAADTRPPRG\r\nAVVAISPWNFPLAIFTGQVAAALMAGNAVLAKPAEQTPIIAALAVRLLHQAGVPETALQL\r\nLPGDGPTVGAALTRDPRVAGVVFTGSTETAQIIARAMAAHLAPGTPLIAETGGLNAMVVD\r\nSTALPEQAVRDVVASAFRSAGQRCSALRCLYVQDDIAPHLIGMLKGAMEELVSGDPARLS\r\nTDVGPVIDAEAKAGIETYLAANKARILHRSTAPEGGHFVAPALLQVGGIADLEREIFGPV\r\nLHLATFAAEDLPAVIAAINARGYGLTFGLHSRIDARVETVAETIRAGNIYVNRNQIGAVV\r\nGSQPFGGEGLSGTGPKAGGPLYLNRFYAPEPVVAVGGWTEAATPILPEARETQLDEIFLP\r\nGPTGELNRLTRHQRGPILCLGPGAEAASAQAAAVVALGGQAVQASGAVSPKALETLTPLA\r\nGVLWWGAAEMGRAYAQALAVRPGPLVPLITAKPDLAHVAHERHLCVDTTAAGGNAALLAG",
          "organism": "Escherchia coli, Rhodobacter capsulatus",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 125.528,
          "total_mw": 251.056,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "Mizzou",
            "address": "Columbia, MO, USA",
            "full_name": "University of Missouri-Columbia",
            "webpage": "http://missouri.edu/"
          }
        ],
        "contributor_name": "John",
        "contributor_surname": "Tanner",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2012-10-16",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.5,
    "number_of_frames": 2,
    "wavelength": 0.1,
    "sample_detector_distance": 1.5,
    "concentration_min": null,
    "concentration_max": 6.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.112,
    "s_max": 3.165,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "GNOM",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of a chimeric EcRHH-RcPutA protein fusion in 50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH 7.5 were collected on the 12.3.1 (SIBYLS) camera on the storage ring Advanced Light Source (ALS) (Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and  2θ is the scattering angle). A solute concentrations of 6 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The merged SAXS curve displayed in this entry was obtained by merging the low-s region (points 2-140, s=0.1125 — 0.9517 nm-1) from an image collected with an exposure time of 0.5 s with the higher-s region (points 19-505, s=0.2159 — 3.1710 nm-1) of a profile collected with exposure time of 2.0 s. The errors in PDDF Rg and I0 were estimated by running GNOM via primus/qt (r4556) assuming Dmax in the range of 173-193 and using all points of the scattering curve (1-503).",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 251.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 3033.0,
  "pddf_i0_error": 5.5,
  "guinier_i0": 3011.44,
  "guinier_i0_error": 11.0,
  "pddf_rg": 5.41,
  "pddf_rg_error": 0.008,
  "guinier_rg": 5.2,
  "guinier_rg_error": 0.02,
  "pddf_dmax": 18.3,
  "pddf_dmax_error": 1.0,
  "porod_volume": 308.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 23,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDB27_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-05-18T10:07:55.648439+02:00",
  "bragg_peak": []
}

{
  "code": "SASDB27",
  "status": "success",
  "started_at": "2026-08-11T15:20:00.476624+00:00",
  "finished_at": "2026-08-11T15:20:08.500214+00:00",
  "source_last_modified": "2026-05-18T10:07:55.648439+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDB27/",
      "path": "/data/saxsdb/sasbdb/entries/27/sasdb27/source/summary.json",
      "size": 7715,
      "sha256": "d217009d7bfc5ab4d5862579a1df7291f1d743062b61d74cd3f51b0f071030e9"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDB27.dat",
      "path": "/data/saxsdb/sasbdb/entries/27/sasdb27/source/SASDB27.dat",
      "size": 22247,
      "sha256": "cf19334b2f40fd699f510b460587ab94ce7d41a99aa29667bf2e952eaaea38a1",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDB27.out",
      "path": "/data/saxsdb/sasbdb/entries/27/sasdb27/source/SASDB27.out",
      "size": 40408,
      "sha256": "91029ebc330c5238c45d824e7a5a7df5ab9f29ccbb460c02e14f7147f4a6e106",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB27.sascif",
      "path": "/data/saxsdb/sasbdb/entries/27/sasdb27/source/SASDB27.sascif",
      "size": 59238,
      "sha256": "3b870352e02875c08e157d06eeb18ed7c4c961719be61db081a1b30f59f14c79",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDB27.zip",
      "path": "/data/saxsdb/sasbdb/entries/27/sasdb27/source/SASDB27.zip",
      "size": 16647,
      "sha256": "c54d186130bde009764e546960b76ef9f7f30884d9c77c333078dd1531c9319a",
      "role": "full_entry_zip",
      "zip_member_count": 2,
      "zip_validation": "central_directory_readable"
    }
  ]
}