Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct

{
  "code": "SASDB54",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.",
    "publication": {
      "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.",
      "author_list": "Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC",
      "journal": "Arch Biochem Biophys",
      "doi": "10.1016/j.abb.2016.04.008",
      "pmid": "27103305",
      "published_date": "2016 Jun 15"
    },
    "status": "released",
    "submitted_date": "2016-02-21",
    "released_date": "2016-08-01"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDB54.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB54.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB54_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB54.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "20Hz",
        "name": "Pilatus 300K",
        "resolution": null
      },
      "name": "Brazilian Synchrotron Light Laboratory",
      "city": "Campinas",
      "country": "Brazil",
      "beamline_name": "SAXS1 Beamline",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Stress-induced protein sti1",
          "short_name": "Hop/Sti1",
          "sequence": "MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDADKCISIKPNW\r\nAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSREARDPIARVFTPEAFRKIQE\r\nNPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTLMYLSGMKIPNDDEDDEEERPSAKAAAEAKA\r\nKEEKKLLTDNEKEAMALKEEGNKLYLSKRFEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITE\r\nCERGIEHGRENHCDYTIVAKLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVE\r\nEAYIDPEIARQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\r\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASGQSADGDEAAR\r\nRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISAGIIRFGQ",
          "organism": "Leishmania braziliensis",
          "uniprot_code": "A4H5F0",
          "uniprot_range_first": 1,
          "uniprot_range_last": 547,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDA\nDKCISIKPNWAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSR\nEARDPIARVFTPEAFRKIQENPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTL\nMYLSGMKIPNDDEDDEEERPSAKAAAEAKAKEEKKLLTDNEKEAMALKEEGNKLYLSKRF\nEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITECERGIEHGRENHCDYTIVAK\nLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIAR\nQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASG\nQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISA\nGIIRFGQ",
          "mw": 62.452,
          "total_mw": 62.452,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol",
        "concentration_unit": "mM",
        "comment": null,
        "additive": "100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol",
        "concentration": 25.0,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "São Carlos - SP - Brasil",
            "full_name": "Instituto de Química de São Carlos",
            "webpage": ""
          }
        ],
        "contributor_name": "Júlio",
        "contributor_surname": "Borges",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": "mg/ml",
    "date": "2016-02-21",
    "storage_temperature": null,
    "cell_temperature": null,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": 0.8,
    "concentration_max": 1.8,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.123,
    "s_max": 4.067,
    "total_exposure_time": null,
    "seccolumn": null
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          "software": "DAMFILT",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDB54_fit1_model1.pdb",
          "model_mw": 58.95,
          "bead_radius": 1.9,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 100
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB54_fit1_fit_img.png",
      "software": null,
      "chi_square_value": 3.549,
      "p_value": 0.0,
      "fit_residual_plot": "SASDB54_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDB54_fit1.fir",
      "fit_log": null,
      "software_version": null,
      "description": null
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "X-ray synchrotron radiation scattering data from solutions of stress-induced protein sti1 (Hop/Sti1) in 25 mM Tris 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol were collected on the SAXS1 and SAXS2 beam lines at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate and Pilatus 300k detectors (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 0.80-1.80 mg/ml were measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged to yield the final composite scattering curve. ",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 63.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 59.0,
  "porod_mw_error": null,
  "pddf_i0": 0.0632,
  "pddf_i0_error": 0.0002,
  "guinier_i0": 0.063,
  "guinier_i0_error": null,
  "pddf_rg": 4.68,
  "pddf_rg_error": 0.02,
  "guinier_rg": 4.51,
  "guinier_rg_error": 0.05,
  "pddf_dmax": 18.0,
  "pddf_dmax_error": null,
  "porod_volume": 94.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 3,
  "guinier_point_last": 16,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDB54_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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