Human Proliferating Cell Nuclear Antigen (PCNA)

{
  "code": "SASDBD7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
    "publication": {
      "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
      "author_list": "Prakash A, Moharana K, Wallace SS, Doublié S",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkw1282",
      "pmid": "27994037",
      "published_date": "2017 Mar 17"
    },
    "status": "released",
    "submitted_date": "2016-11-04",
    "released_date": "2017-01-03"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBD7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBD7.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBD7_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBD7_kratky_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Proliferating cell nuclear antigen",
          "short_name": null,
          "sequence": "MHHHHHHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFACICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS",
          "organism": "Homo sapiens",
          "uniprot_code": "P12004",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "trimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY\nRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD\nLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI\nKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK\nIADMGHLKYYLAPKIEDEEGS",
          "mw": 29.669,
          "total_mw": 89.007,
          "number_molecules": 3,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25mM HEPES 100mM NaCl 1mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Human Proliferating Cell Nuclear Antigen (PCNA)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Burlington, VT, USA",
            "full_name": "University of Vermont",
            "webpage": "https://www.uvm.edu/"
          }
        ],
        "contributor_name": "Kedar",
        "contributor_surname": "Moharana",
        "orcid": null
      }
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-01-20",
    "storage_temperature": 4.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.2,
    "number_of_frames": 24,
    "wavelength": 0.1127,
    "sample_detector_distance": 1.4,
    "concentration_min": 1.0,
    "concentration_max": 6.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.164,
    "s_max": 5.285,
    "total_exposure_time": null,
    "seccolumn": null
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          "user": 177
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  "pddf_software_version": "ATSAS GNOM 5.0",
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  "description": "Experimental MW reported here is from SAXSMoW. PCNA was used to calibrate forward intensity for MW estimation of other scatterers.",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 85.9,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 75.414,
  "porod_mw_error": null,
  "pddf_i0": 486.2,
  "pddf_i0_error": 1.008,
  "guinier_i0": 483.2,
  "guinier_i0_error": 1.4,
  "pddf_rg": 3.439,
  "pddf_rg_error": 0.052,
  "guinier_rg": 3.44,
  "guinier_rg_error": 0.025,
  "pddf_dmax": 9.72,
  "pddf_dmax_error": 0.18,
  "porod_volume": 128.2,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 39,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBD7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T16:32:30.058203+00:00",
  "finished_at": "2026-08-11T16:32:38.918350+00:00",
  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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