SASDBF3

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Concentration— – — Buffer / pH25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol / 7.5
Experimental temperature— Facility / beamlineBrazilian Synchrotron Light Laboratory / SAXS2 Beamline
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains
View sequence
MGSSHHHHHHSSGLVPRGSHMTETFAFQAEINQLMSLIINTFYSNKEIFLRELISNASDACDKIRYQSLTDPSVLGEETHLRVRVVPDKANKTLTVEDNGIGMTKADLVNNLGTIARSGTKAFMEALEAGGDMSMIGQFGVGFYSAYLVADRVTVVSKNNSDEAYVWESSAGGTFTITSVPESDMKRGTRITLHLKEDQQEYLEERRVKELIKKHSEFIGYDIELMVEKTAEKEVTDEDEEEDESKKKSCGDEGEPKVEEVTEGGEDKKKKTKKVKEVTKTYEVQNKHKPLWTRDPKDVTKEEYAAFYKAISNDWEDPAATKHFSVEGQLEFRAIAFVPKRAPFDMFEPNKKRNNIKLYVRRVFIMDNCEDLCPDWLGFVKGVVDSEDLPLNISRENLQQNKILKVIRKNIVKKCLELFEEIAENKEDYKQFYEQFGKNIKLGIHEDTANRKKLMELLRFYSTESGEEMTTLKDYVTRMKPEQKSIYYITGDSKKKLESSPFIEKARRCGLEVLFMTEPIDEYVMQQVKDFEDKKFACLTKE
proteinLeishmania braziliensis——–—monomerMolecule count 162.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1227[2]0.1906827–3.297047 1/nmNo uncertainty columnMissing 2270sasbdb/entries/f3/sasdbf3/source/SASDBF3.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.0—nm
i0Guinier0.0013——
i0P(r)0.1311——
mwExperimental67.0—kDa
porod_volumePorod89.0—nm³
rgGuinier3.15—nm
rgP(r)3.24—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanolBuffer concentration25.0 mM
pH7.5Additives100 mM NaCl, 1 mM 2-mercaptoethanol
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2011-09-01Storage / measurement temperature— / —
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 165 CCD
Facility / beamlineBrazilian Synchrotron Light Laboratory / SAXS2 Beamline · Campinas, Brazil
q range0.191 – 3.297Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:184 field values
Field pathRaw value
codeSASDBF3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleInsights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.
project.publication.titleInsights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.
project.publication.author_listSeraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC
project.publication.journalInt J Biol Macromol
project.publication.doi10.1016/j.ijbiomac.2017.01.058
project.publication.pmid28104372
project.publication.published_date2017 Apr
project.statusreleased
project.submitted_date2016-01-18
project.released_date2016-01-21
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 165 CCD
experiment.instrument.detector.resolutionnull
experiment.instrument.nameBrazilian Synchrotron Light Laboratory
experiment.instrument.cityCampinas
experiment.instrument.countryBrazil
experiment.instrument.beamline_nameSAXS2 Beamline
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameLeishmania braziliensis heat shock protein 90 (Hsp90) N and M domains
experiment.sample.molecule[0].short_nameL. brazilien.Hsp90NM
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMTETFAFQAEINQLMSLIINTFYSNKEIFLRELISNASDACDKIRYQSLT DPSVLGEETHLRVRVVPDKANKTLTVEDNGIGMTKADLVNNLGTIARSGTKAFMEALEAGGDMSMIGQFG VGFYSAYLVADRVTVVSKNNSDEAYVWESSAGGTFTITSVPESDMKRGTRITLHLKEDQQEYLEERRVKE LIKKHSEFIGYDIELMVEKTAEKEVTDEDEEEDESKKKSCGDEGEPKVEEVTEGGEDKKKKTKKVKEVTK TYEVQNKHKPLWTRDPKDVTKEEYAAFYKAISNDWEDPAATKHFSVEGQLEFRAIAFVPKRAPFDMFEPN KKRNNIKLYVRRVFIMDNCEDLCPDWLGFVKGVVDSEDLPLNISRENLQQNKILKVIRKNIVKKCLELFE EIAENKEDYKQFYEQFGKNIKLGIHEDTANRKKLMELLRFYSTESGEEMTTLKDYVTRMKPEQKSIYYIT GDSKKKLESSPFIEKARRCGLEVLFMTEPIDEYVMQQVKDFEDKKFACLTKE
experiment.sample.molecule[0].organismLeishmania braziliensis
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw62.0
experiment.sample.molecule[0].total_mw62.0
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive100 mM NaCl, 1 mM 2-mercaptoethanol
experiment.sample.buffer.concentration25.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLeishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressSão Paulo, SP, Brazil
experiment.contributor[0].affiliation[0].full_nameInstituto de Física, Universidade de São Paulo
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameLeandro
experiment.contributor[0].contributor_surnameBarbosa
experiment.contributor[0].orcidnull
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experiment.contributor[1].affiliation[0].addressSão Carlos - SP - Brasil
experiment.contributor[1].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[1].affiliation[0].webpage
experiment.contributor[1].contributor_nameJúlio
experiment.contributor[1].contributor_surnameBorges
experiment.contributor[1].orcidnull
experiment.contributor[2].affiliation[0].short_namenull
experiment.contributor[2].affiliation[0].addressSão Carlos - SP - Brasil
experiment.contributor[2].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[2].affiliation[0].webpage
experiment.contributor[2].contributor_namePaulo
experiment.contributor[2].contributor_surnameDores-Silva
experiment.contributor[2].orcidnull
experiment.contributor[3].affiliation[0].short_namenull
experiment.contributor[3].affiliation[0].addressSão Carlos - SP - Brasil
experiment.contributor[3].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[3].affiliation[0].webpage
experiment.contributor[3].contributor_nameThiago
experiment.contributor[3].contributor_surnameSeraphim
experiment.contributor[3].orcidnull
experiment.contributor[4].affiliation[0].short_namenull
experiment.contributor[4].affiliation[0].addressSão Carlos - SP - Brasil
experiment.contributor[4].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[4].affiliation[0].webpage
experiment.contributor[4].contributor_nameKelly
experiment.contributor[4].contributor_surnameSilva
experiment.contributor[4].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2011-09-01
experiment.storage_temperaturenull
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experiment.exposure_timenull
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experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.191
experiment.s_max3.297
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius3.0
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fits[0].softwarenull
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fits[0].p_value0.001616
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains in 25 mM Tris 100 mM NaCl, 1 mM 2-mercaptoethanol were collected on the SAXS2 Beamline camera on the storage ring Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate detector (s = 4π sin θ/λ, where 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve.
tags[]
intensity_unitnull
experimental_mw67.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.1311
pddf_i0_errornull
guinier_i00.0013
guinier_i0_errornull
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pddf_rg_errornull
guinier_rg3.15
guinier_rg_errornull
pddf_dmax13.0
pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
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guinier_point_last17
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBF3_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBF3
statussuccess
started_at2026-08-11T13:58:53.457443+00:00
finished_at2026-08-11T13:59:03.193350+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDBF3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.",
    "publication": {
      "title": "Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.",
      "author_list": "Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC",
      "journal": "Int J Biol Macromol",
      "doi": "10.1016/j.ijbiomac.2017.01.058",
      "pmid": "28104372",
      "published_date": "2017 Apr"
    },
    "status": "released",
    "submitted_date": "2016-01-18",
    "released_date": "2016-01-21"
  },
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    "sample": {
      "molecule": [
        {
          "long_name": "Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains",
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View the complete manifest.json source
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