SASDBL2

MBP-PICK1 fusion

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

This dataset was collected at a concentration of 3.75 mg/ml.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · MBP-PICK1 fusion

浓度0.46 – 7.5 缓冲液 / pH50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT / 7.5
实验温度4.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1
波长— nm曝光40.0 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Maltose Binding Protein fused to Protein Interacting with C kinase 1
查看序列
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKGGSWCDS
proteinHomo sapiensQ9NRD5—–—dimer分子数 287.089 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1467[3]0.103426–2.93517 1/nm含误差列缺失 079sasbdb/entries/l2/sasdbl2/source/SASDBL2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)27.6—nm
i0Guinier41.42——
i0P(r)41.58——
mwExperimental172.2—kDa
porod_volumePorod483.0—nm³
rgGuinier8.38—nm
rgP(r)8.7—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT缓冲液浓度—
pH7.5添加剂300 mM NaCl, 1 mM maltose, 1 mM EGTA, 2 mM DTT
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-10-13储存 / 测量温度— / 4.0
曝光时间40.0帧数20
波长—样品-探测器距离—
光源X-ray synchrotron探测器Finger Lakes CCD
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1 · Ithaca, NY, USA
q 范围0.103 – 2.935样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/l2/sasdbl2/source/SASDBL2.datdownloaded21795ec2f2c4e44972403256507111f1f2567c8250537423d8d7ee5709896b454b71f下载查看原文件源站
full_entry_zipsasbdb/entries/l2/sasdbl2/source/SASDBL2.zipdownloaded255070e454ae651214f6537b9ce1ac2fd49aa1a10440cec92fc100b1e39c1a47d44232下载查看原文件源站
pddfsasbdb/entries/l2/sasdbl2/source/SASDBL2.outdownloaded2091415dfa52f76e4786ba7553cf6c7a27cdd7ce9be741822989cf249921cf3d72238下载查看原文件源站
sascifsasbdb/entries/l2/sasdbl2/source/SASDBL2.sascifdownloaded13175714742660002d0e0a1de664dfe8832111fd4811f081bc274112c4d9ad6ae74bd12下载查看原文件源站
summarysasbdb/entries/l2/sasdbl2/source/summary.jsondownloaded7638e28a3370ec68625a414eab2d1170d62117af25cb65e2b635062a80a8ac3a1e49下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBL2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titlePICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.
project.publication.titlePICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.
project.publication.author_listMadasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R
project.publication.journalMol Biol Cell
project.publication.doi10.1091/mbc.E14-10-1448
project.publication.pmid25657323
project.publication.published_date2015 Apr 1
project.statusreleased
project.submitted_date2015-10-13
project.released_date2016-04-15
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBL2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDBL2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDBL2.sascif
experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameFinger Lakes CCD
experiment.instrument.detector.resolution0.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameG1
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMaltose Binding Protein fused to Protein Interacting with C kinase 1
experiment.sample.molecule[0].short_nameMBP-PICK1
experiment.sample.molecule[0].sequenceMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDI IFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNK DLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIK DVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSK VNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL GAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDA ALAAAQTNAAAMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYI VQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPK QGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEH TKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIK PMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVST GNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCY AVLRDADVFPIEVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKG GSWCDS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9NRD5
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAAL DGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKK VKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEHTKNLLRAFYEL SQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLN KAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRC RQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPI EVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKGGSWCDS
experiment.sample.molecule[0].mw87.089
experiment.sample.molecule[0].total_mw174.178
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive300 mM NaCl, 1 mM maltose, 1 mM EGTA, 2 mM DTT
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMBP-PICK1 fusion
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameRoberto
experiment.contributor[0].contributor_surnameDominguez
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-10-13
experiment.storage_temperaturenull
experiment.cell_temperature4.0
experiment.exposure_time40.0
experiment.number_of_frames20
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_min0.46
experiment.concentration_max7.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.103
experiment.s_max2.935
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDBL2_fit1_model1_img.png
fits[0].models[0].softwareCRYSOL
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
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fits[0].models[0].model_mwnull
fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user87
fits[0].fit_unit1/A
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fits[0].softwarenull
fits[0].chi_square_value1.1881
fits[0].p_value0.0
fits[0].fit_residual_plotfit4columns358_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDBL2_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionThis dataset was collected at a concentration of 3.75 mg/ml.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw172.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i041.58
pddf_i0_errornull
guinier_i041.42
guinier_i0_errornull
pddf_rg8.7
pddf_rg_errornull
guinier_rg8.38
guinier_rg_errornull
pddf_dmax27.6
pddf_dmax_errornull
porod_volume483.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBL2_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBL2
statussuccess
started_at2026-08-11T13:40:45.692213+00:00
finished_at2026-08-11T13:40:55.673814+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBL2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.",
    "publication": {
      "title": "PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.",
      "author_list": "Madasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R",
      "journal": "Mol Biol Cell",
      "doi": "10.1091/mbc.E14-10-1448",
      "pmid": "25657323",
      "published_date": "2015 Apr 1"
    },
    "status": "released",
    "submitted_date": "2015-10-13",
    "released_date": "2016-04-15"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBL2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBL2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL2_dat_img.png",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBL2.sascif",
  "experiment": {
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        "type": "CCD",
        "name": "Finger Lakes CCD",
        "resolution": 0.0
      },
      "name": "Cornell High Energy Synchrotron Source (CHESS)",
      "city": "Ithaca, NY",
      "country": "USA",
      "beamline_name": "G1",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Maltose Binding Protein fused to Protein Interacting with C kinase 1",
          "short_name": "MBP-PICK1",
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        "concentration_unit": null,
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        "concentration": null,
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            "address": "Philadelphia, PA, United States",
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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