SASDBN4

Callose synthase

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Callose synthase

Concentration— – 1.0 Buffer / pHTris, 50 mM NaCl / 7.3
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.12 nmExposure0.05 s × 18

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Callose synthase
View sequence
MASRGSHHHHHHGAGDRGPEFELGTRGSCAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFGNKFKDGIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWDVTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLVGLVNDEETDSGRVVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASKLLFQNAIRLPDASNEDFYRQVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSEEVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSELWTTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRNLQGELGGQSDGFVSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMKQNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTILGVRHAHGLSAWSHPQFEK
proteinArabidopsis thalianaQ9ZT82570–1208octamerMolecule count 879.092 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11808[3]0.0269502–4.80121 1/nmHas uncertainty columnMissing 0478sasbdb/entries/n4/sasdbn4/source/SASDBN4.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)30.03.0nm
i0Guinier25374.1—arbitrary
mwExperimental623.0—kDa
mwPorod646.0—kDa
porod_volumePorod1033.34—nm³
rgGuinier7.990.34nm
rgP(r)7.94—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer nameTris, 50 mM NaClBuffer concentration—
pH7.3Additives50 mM NaCl
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuterationFalse / —

Acquisition Conditions and Instrument

Measurement date2015-06-02Storage / measurement temperature20.0 / 10.0
Exposure time0.05Frames18
Wavelength0.12Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.027 – 4.801Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zip:source record and ZIP contents (6 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:186 field values
Field pathRaw value
codeSASDBN4
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural Characterization of Cell Wall and Plasma Membrane Proteins of Arabidopsis thaliana
project.publication.titleStructural Characterization of Cell Wall and Plasma Membrane Proteins of Arabidopsis thaliana
project.publication.author_listHaifa El Kilani
project.publication.journalUniversity of Hamburg Dissertation
project.publication.doinull
project.publication.pmidnull
project.publication.published_date2016 June
project.statusreleased
project.submitted_date2016-03-24
project.released_date2020-09-29
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameCallose synthase
experiment.sample.molecule[0].short_nameAtGSLO5-IL
experiment.sample.molecule[0].sequenceMASRGSHHHHHHGAGDRGPEFELGTRGSCAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFGNKFKD GIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWDVTVIRWPCFLLCNELLLAL SQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPK IYETLQKLVGLVNDEETDSGRVVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASKLLFQNAIRLPDASNEDFYRQ VRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSEEVVYSKEQLRNETEDGISTLY YLQTIYADEWKNFKERMHREGIKTDSELWTTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQ ELGSVRNLQGELGGQSDGFVSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMKQ NEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYF EEALKMRNLLQEYNHYHGIRKPTILGVRHAHGLSAWSHPQFEK
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeQ9ZT82
experiment.sample.molecule[0].uniprot_range_first570
experiment.sample.molecule[0].uniprot_range_last1208
experiment.sample.molecule[0].oligomerizationoctamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSLRHRTVPPQTGRPLAAEAVGIEEEPYNIIPVNNLLADHPSLRFPEVRAAAAALKTVGD LRRPPYVQWRSHYDLLDWLALFFGFQKDNVRNQREHMVLHLANAQMRLSPPPDNIDSLDS AVVRRFRRKLLANYSSWCSYLGKKSNIWISDRNPDSRRELLYVGLYLLIWGEAANLRFMP ECICYIFHNMASELNKILEDCLDENTGQPYLPSLSGENAFLTGVVKPIYDTIQAEIDESK NGTVAHCKWRNYDDINEYFWTDRCFSKLKWPLDLGSNFFKSRGKSVGKTGFVERRTFFYL YRSFDRLWVMLALFLQAAIIVAWEEKPDTSSVTRQLWNALKARDVQVRLLTVFLTWSGMR LLQAVLDAASQYPLVSRETKRHFFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNA ATTKIYQFLYAVGAFLVPEILALALFIIPWMRNFLEETNWKIFFALTWWFQGKSFVGRGL REGLVDNIKYSTFWIFVLATKFTFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYGDSNRF SVALLWLPVVLIYLMDIQIWYAIYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQF NLMPEEQLLNARGFGNKFKDGIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFR EEDIVSDREVELLELPKNSWDVTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICK NEYRRCAVVEAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPK IYETLQKLVGLVNDEETDSGRVVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASK LLFQNAIRLPDASNEDFYRQVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPH APQVEKMMAFSVLTPYYSEEVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHRE GIKTDSELWTTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQ ELGSVRNLQGELGGQSDGFVSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIY GSQKAKKEPQAEEILYLMKQNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFR VKLPGPVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIR KPTILGVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFL SRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASG NGEQVLSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEK SALADSTDTNAALGVILNQQFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAV FYTFSMGTRAHYFGRTILHGGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLIL IVYASHSPIAKDSLIYIAMTITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWY QGRISTKSEQSWEKWWYEEQDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTS LFVYLFSWIYIFAIFVLFLVIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHF SFIDIFTSLLAFIPTGWGILLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVA FLSWMPGFQSMQTRILFNEAFSRGLRIMQIVTGKKSKGDV
experiment.sample.molecule[0].mw79.092
experiment.sample.molecule[0].total_mw632.736
experiment.sample.molecule[0].number_molecules8
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.nameTris, 50 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive50 mM NaCl
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.3
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCallose synthase
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixtureFalse
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHamburg, Germany
experiment.contributor[0].affiliation[0].full_nameUniversity of Hamburg
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-hamburg.de/
experiment.contributor[0].contributor_nameHaifa
experiment.contributor[0].contributor_surnameEl Kilani
experiment.contributor[0].orcidnull
experiment.contributor[1].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[1].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[1].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[1].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[1].contributor_nameAl
experiment.contributor[1].contributor_surnameKikhney
experiment.contributor[1].orcidhttps://orcid.org/0000-0003-1321-3956
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-06-02
experiment.storage_temperature20.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames18
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.027
experiment.s_max4.801
experiment.total_exposure_timenull
experiment.seccolumnnull
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pddf_softwareATSAS GNOM
pddf_software_versionGNOM 5
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw623.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw646.0
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i025374.1
guinier_i0_errornull
pddf_rg7.94
pddf_rg_errornull
guinier_rg7.99
guinier_rg_error0.34
pddf_dmax30.0
pddf_dmax_error3.0
porod_volume1033.34
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last52
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBN4_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBN4
statussuccess
started_at2026-08-11T14:27:13.792282+00:00
finished_at2026-08-11T14:27:26.058410+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDBN4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
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