SASDBQ7

Domain 3'X of hepatitis C virus RNA at higher ionic strength

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2024-09-11T17:08:26.026510+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Domain 3'X of hepatitis C virus RNA at higher ionic strength

Concentration0.37 – 1.5 Buffer / pH10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl / 7.0
Experimental temperature27.0 Facility / beamlineCenter for Cancer Research, National Cancer Institute / Rigaku BioSAXS-2000
Wavelength0.154 nmExposure1.0 s × 30

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Domain 3'X of hepatitis C virus
View sequence
GGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCUUGACUGCAGAGAGUGCUGAUACUGGCCUCUCUGCAGAUCAAGU
RNAHepatitis C virus——–—dimerMolecule count 231.7 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1501[3]0.0–1.0 1/AHas uncertainty columnMissing 0159sasbdb/entries/q7/sasdbq7/source/SASDBQ7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)24.40.06Å
i0Guinier89.690.2—
i0P(r)91.70.0—
mwExperimental81.9—kDa
rgGuinier5.9920.022Å
rgP(r)6.4770.0Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaClBuffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-11-20Storage / measurement temperature27.0 / 27.0
Exposure time1.0Frames30
Wavelength0.154Sample-to-detector distance0.5
SourceX-ray in houseDetectorPilatus 100K
Facility / beamlineCenter for Cancer Research, National Cancer Institute / Rigaku BioSAXS-2000 · Frederick, MD, USA
q range0.0 – 10.0Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDBQ7
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/A
project.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.author_listCantero-Camacho Á, Fan L, Wang YX, Gallego J
project.publication.journalRNA
project.publication.doi10.1261/rna.060632.117
project.publication.pmid28630140
project.publication.published_date2017 Sep
project.statusreleased
project.submitted_date2016-11-23
project.released_date2017-06-26
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameCenter for Cancer Research, National Cancer Institute
experiment.instrument.cityFrederick, MD
experiment.instrument.countryUSA
experiment.instrument.beamline_nameRigaku BioSAXS-2000
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
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experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDomain 3'X of hepatitis C virus
experiment.sample.molecule[0].short_name3'X
experiment.sample.molecule[0].sequenceGGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCUUGACUGCAGAGAGUGCUGAUACUGGCCUCUCUGCAGAUCAAGU
experiment.sample.molecule[0].organismHepatitis C virus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw31.7
experiment.sample.molecule[0].total_mw63.4
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl
experiment.sample.buffer.concentration_unitnull
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experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
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experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDomain 3'X of hepatitis C virus RNA at higher ionic strength
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressValencia, Spain
experiment.contributor[0].affiliation[0].full_nameUniversidad Católica de Valencia
experiment.contributor[0].affiliation[0].webpagehttps://www.ucv.es/
experiment.contributor[0].contributor_nameJosé
experiment.contributor[0].contributor_surnameGallego
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-20
experiment.storage_temperature27.0
experiment.cell_temperature27.0
experiment.exposure_time1.0
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experiment.wavelength0.154
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experiment_descriptionSAXS data from solutions of Domain 3'X of hepatitis C virus RNA at higher ionic strength in 10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl, pH 7, were collected on an in-house Rigaku BIOSAXS-2000 instrument (Frederick, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 1.5 mg/ml were measured at 27°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for RNA concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[]
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pddf_i091.7
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guinier_i089.69
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pddf_rg6.477
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symmetrynull
last_modified2024-09-11T17:08:26.026510+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBQ7
statussuccess
started_at2026-08-11T16:41:14.793331+00:00
finished_at2026-08-11T16:41:22.526814+00:00
source_last_modified2024-09-11T17:08:26.026510+02:00
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View the complete summary.json source
{
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  "status": "Published",
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  "angular_unit": "1/A",
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    "title": "Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.",
    "publication": {
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      "author_list": "Cantero-Camacho Á, Fan L, Wang YX, Gallego J",
      "journal": "RNA",
      "doi": "10.1261/rna.060632.117",
      "pmid": "28630140",
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  "experiment_description": "SAXS data from solutions of Domain 3'X of hepatitis C virus RNA at higher ionic strength in 10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl, pH 7, were collected on an in-house Rigaku BIOSAXS-2000 instrument (Frederick, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 1.5 mg/ml were measured at 27°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for RNA concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.",
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBQ7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-09-11T17:08:26.026510+02:00",
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}
View the complete manifest.json source
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