Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)
Data type: SASBDB experimental dataStatus: PublishedCurve type: Single concentrationLast updated: 2019-12-06T13:07:15.624532+01:00
Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.
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1. Samples, Components and Experimental Conditions Sample & Experiment
Sample 1 · Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)
2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
dmax
P(r)
12.6
—
nm
i0
Guinier
0.072
—
—
i0
P(r)
0.072
—
—
mw
Experimental
38.4
—
kDa
mw
Porod
34.0
—
kDa
porod_volume
Porod
57.9
—
nm³
rg
Guinier
3.3
—
nm
rg
P(r)
3.35
—
nm
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
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3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
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4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/x2/sasdbx2/source/SASDBX2.dat
downloaded
25776
full_entry_zip
sasbdb/entries/x2/sasdbx2/source/SASDBX2.zip
downloaded
22079
pddf
sasbdb/entries/x2/sasdbx2/source/SASDBX2.out
downloaded
28031
sascif
sasbdb/entries/x2/sasdbx2/source/SASDBX2.sascif
downloaded
55167
summary
sasbdb/entries/x2/sasdbx2/source/summary.json
downloaded
6696
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5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
X-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.
Related Publication
Title
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.
Authors
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
DOI / PMID
10.1016/j.str.2015.12.001 / 26774128
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.
experiment_description
X-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.