SASDBX2

Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00

Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

Concentration— – — Buffer / pH50 mM HEPES 100mM NaCl 1mM TCEP / 7.5
Experimental temperature15.0 Facility / beamlineSOLEIL / SWING
Wavelength0.1 nmExposure1.5 s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex
View sequence
GSRIYDNTALLNKPVHNEKLSFTWDPIVHQSGHGVILEGTVVDFSRHAITDVKNRKERYNVLYIKPSRVHRRKYDSKGNEIEPNFSDTKKVNTGFLMSSFKVEAKGETDCLDERQLREIVNKEQLVKVTIKHCPREAFAFWISEAEMDKTELEPGQEVRLKTKGDGPFIFSSAKLDSGTVTKCNFAGDENAGASWTEKIMANKSNQENTGKSAAQGEGADDDEWDDGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAAL
proteinDanio rerio / Homo sapiens——–—monomerMolecule count 143.018 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1559[3]0.152458–2.48064 1/nmHas uncertainty columnMissing 00sasbdb/entries/x2/sasdbx2/source/SASDBX2.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.6—nm
i0Guinier0.072——
i0P(r)0.072——
mwExperimental38.4—kDa
mwPorod34.0—kDa
porod_volumePorod57.9—nm³
rgGuinier3.3—nm
rgP(r)3.35—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM HEPES 100mM NaCl 1mM TCEPBuffer concentration50.0 mM
pH7.5Additives100mM NaCl, 1mM TCEP
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2014-12-04Storage / measurement temperature15.0 / 15.0
Exposure time1.5Frames—
Wavelength0.1Sample-to-detector distance1.82
SourceX-ray synchrotronDetectorAVIEX PCCD170170
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.152 – 2.481Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDBX2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleHybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.
project.publication.titleHybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.
project.publication.author_listFetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
project.publication.journalStructure
project.publication.doi10.1016/j.str.2015.12.001
project.publication.pmid26774128
project.publication.published_date2016 Feb 2
project.statusreleased
project.submitted_date2015-12-03
project.released_date2016-07-03
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameZebrafish arpin/human tankyrase 2 ankyrin repeat domain complex
experiment.sample.molecule[0].short_nameArpin/tankyrase
experiment.sample.molecule[0].sequenceGSRIYDNTALLNKPVHNEKLSFTWDPIVHQSGHGVILEGTVVDFSRHAITDVKNRKERYNVLYIKPSRVHRRKYDSKGNEIEPNFSDTKKVNTGFLMSSFKVEAKGETDCLDERQLREIVNKEQLVKVTIKHCPREAFAFWISEAEMDKTELEPGQEVRLKTKGDGPFIFSSAKLDSGTVTKCNFAGDENAGASWTEKIMANKSNQENTGKSAAQGEGADDDEWDD GNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAAL
experiment.sample.molecule[0].organismDanio rerio / Homo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw43.018
experiment.sample.molecule[0].total_mw43.018
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateTrue
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM HEPES 100mM NaCl 1mM TCEP
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive100mM NaCl, 1mM TCEP
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameZebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameSoleil
experiment.contributor[0].affiliation[0].addressSaint-Aubin, France
experiment.contributor[0].affiliation[0].full_nameSoleil Synchrotron
experiment.contributor[0].affiliation[0].webpagehttp://www.synchrotron-soleil.fr/
experiment.contributor[0].contributor_nameAurélien
experiment.contributor[0].contributor_surnameThureau
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-12-04
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.5
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experiment.seccolumnnull
fits[]
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i0_calibration_standardnull
descriptionDue to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.
tags[]
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pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBX2_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBX2
statussuccess
started_at2026-08-11T13:48:19.628456+00:00
finished_at2026-08-11T13:48:27.007104+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDBX2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.",
    "publication": {
      "title": "Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.",
      "author_list": "Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J",
      "journal": "Structure",
      "doi": "10.1016/j.str.2015.12.001",
      "pmid": "26774128",
      "published_date": "2016 Feb 2"
    },
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
View the complete manifest.json source
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