Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex

{
  "code": "SASDC73",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.",
    "publication": {
      "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.",
      "author_list": "Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH",
      "journal": "Structure",
      "doi": "10.1016/j.str.2015.02.012",
      "pmid": "25817386",
      "published_date": "2015 May 5"
    },
    "status": "released",
    "submitted_date": "2017-05-18",
    "released_date": "2017-05-05"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC73.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC73.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC73_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC73.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Serine/threonine-protein kinase ATG1",
          "short_name": "Atg1",
          "sequence": "DSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDYDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQ\r\nTLTSATTKISSATNVDTQIPAPELKKLCTESLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQ\r\nWIRDRFNECLDKAEFLRLKLHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCEL\r\nAYATSLWMLEILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CSX2",
          "uniprot_range_first": 562,
          "uniprot_range_last": 831,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSSESHDKVVAKAIRLPTENYSVEKEIGKGSFAVVYKGLSLRDGRNIAIKAVSRSKLKNK\nKLLENLEVEIAILKKIKHPHIVGLIDCERTSSDFYLIMEYCALGDLTFFIKKRKNLVLKH\nPLIKTVFEHYPPPSTEHNGLNRVLVVNYLQQLSSALKFLRSKNLVHRDIKPQNLLLCTPL\nLDYNDPKTFHELGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKY\nNAKADLWSVGTVLYEMCCGRPPFKASNHLELFQKIKKANDEITVPSNCYIEPKLFNLIRG\nLLTFDPDSRMGFTDFFNNEVVTEDLTRYEQSYEPDLESKSKDVAESNMFVSEYLVKPLKQ\nQESAHIPPTQTDENTSVQTGVRRTSGKERLATNHPPHQQIHPEDNSQNPEQSYQSASQKR\nLKSSYNDLILEKEYVVVEKKTVEVNSLADDFANNGPITNNQGAQVIKPLRYRTSSSSDAS\nGGRRASLVERRLSISSLSPSNALSKALGLASVRLFGYQHNTKATSSPPQQTLLNPQIFQE\nLTENAVLRADHKLNPFSEQMLDSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDY\nDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQTLTSATTKISSATNVDTQIPAPELKKLCTE\nSLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQWIRDRFNECLDKAEFLRLKL\nHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCELAYATSLWMLE\nILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV",
          "mw": 30.664,
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Autophagy-related protein 13",
          "short_name": "Atg13",
          "sequence": "FDRTAKPRKSTENPPEDLLEF\r\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNV",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CWK2",
          "uniprot_range_first": 400,
          "uniprot_range_last": 475,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSEGEVQVIELINNFFLKSALLLEQSKVAHNFDTEEALRDGNHLFNIETRGDPLLEAQIQ\nPWITFDGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVE\nLDKQTIDDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDIITKPHNSQQPALINVQTRL\nLDGSKPILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKTTFGSLRITVSYRKD\nVDFYVIDSDDLQKRYMTPSLSNETTTVPDRRASSNCSRSMSVSPKTNTINATLFPLEGSS\nARRQSISSKLQPFKVGSVGSGSFVQSGSAQSTTSLVPSLSRNVSSSSVVAALKVQRGSAG\nSTIVNGDVPPELSSVGSGSKYSSSFGRIRRHSSIRRSESFDRTAKPRKSTENPPEDLLEF\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNVRFGSN\nSHSPIPSFSPNYGSIPSRLSQGSRNNSNAELITSRKSSLDRHKHNLLSRTGSNVDINRRG\nSVGTMETTNEDSKEDEQSHMKGMHFSNEATINKDDDEDEMLMKRSFNAGTSTTEQVMGSP\nRSIRSISVSSYPRNQLPLKHLNLSHPTTSATTTHAKFHKSEMSPDPLHTEGAQPHTSSQH\nHNSSQKNDEDDDLLFVMSDMNLTN",
          "mw": 8.726,
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          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Autophagy-related protein 17",
          "short_name": "Atg17",
          "sequence": "MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILNCLVNQVEFFK\r\nNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDLTDDHSSLSDFICMDHVDILQ\r\nQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLRTYFDSHFSRDSKNNLFKLLESDYVTELNEF\r\nENELADFLRSITDHFDKCTILKEQQIPLPDLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETI\r\nSEIMDKIGGFHLLASKIVTELEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLL\r\nRERDRRKATALQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\r\nVPN",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CS99",
          "uniprot_range_first": 1,
          "uniprot_range_last": 423,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILN\nCLVNQVEFFKNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDL\nTDDHSSLSDFICMDHVDILQQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLR\nTYFDSHFSRDSKNNLFKLLESDYVTELNEFENELADFLRSITDHFDKCTILKEQQIPLPD\nLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETISEIMDKIGGFHLLASKIVTE\nLEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLLRERDRRKATA\nLQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\nVPN",
          "mw": 49.864,
          "total_mw": 99.728,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Autophagy-related protein 29",
          "short_name": null,
          "sequence": "MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGAPEPFLKKRSY\r\nTLFQNQLKVLSNQID",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CTU8",
          "uniprot_range_first": 1,
          "uniprot_range_last": 85,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGA\nPEPFLKKRSYTLFQNQLKVLSNQIDVTSNTRSSSESRNSVDNNILQNLQASRIMNHKLDK\nTDNLANNQESSSELSNLSVSKSALEDALMDCLQL",
          "mw": 10.031,
          "total_mw": 20.062,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "KLLA0A10637p",
          "short_name": "Atg31",
          "sequence": "MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLDYENVIIIDSE\r\nPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGLDNLSKLYHERNAQIRKLLDS\r\nIAP",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CX74",
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          "uniprot_range_last": 143,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLD\nYENVIIIDSEPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGL\nDNLSKLYHERNAQIRKLLDSIAP",
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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      "buffer": {
        "name": "20 mM Tris, 200 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Hanover, NH, USA",
            "full_name": "Dartmouth College",
            "webpage": "http://home.dartmouth.edu/"
          }
        ],
        "contributor_name": "Michael",
        "contributor_surname": "Ragusa",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2013-12-20",
    "storage_temperature": 10.0,
    "cell_temperature": 22.0,
    "exposure_time": 0.6,
    "number_of_frames": null,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": 0.7,
    "concentration_max": 2.6,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.109,
    "s_max": 2.719,
    "total_exposure_time": null,
    "seccolumn": null
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  "estimated_volume_method": null,
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  "description": null,
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  "tags": [],
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  "experimental_mw_error": null,
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  "pddf_rg_error": 0.1,
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  "pddf_dmax_error": null,
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  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 6,
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  "symmetry": null,
  "last_modified": "2020-11-24T17:34:01.079622+01:00",
  "bragg_peak": []
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