SASDC93

Tetrameric apoptosis regulator BAX (Bcl-2 associated X)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2021-12-03T19:22:36.894202+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Tetrameric apoptosis regulator BAX (Bcl-2 associated X)

Concentration— – 2.0 Buffer / pH20mM sodium phosphate 100mM NaCl / 8.0
Experimental temperature15.0 Facility / beamlineTaiwan Photon Source, NSRRC / 23A
Wavelength0.08266 nmExposure30.0 s × 2

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Apoptosis regulator BAX (Bcl-2 associated X)
View sequence
MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG
proteinHomo sapiensQ078121–192tetramerMolecule count 421.184 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1214[3]0.01444–0.29913 1/AHas uncertainty columnMissing 02sasbdb/entries/93/sasdc93/source/SASDC93.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.96—Å
i0Guinier0.02289230.00032—
i0P(r)0.023540.0002—
mwExperimental85.5—kDa
porod_volumePorod180.0—ų
rgGuinier3.6450.07Å
rgP(r)3.850.004Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM sodium phosphate 100mM NaClBuffer concentration—
pH8.0Additives—
Buffer description20mM sodium phosphate, 100mM NaCl
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-05-28Storage / measurement temperature— / 15.0
Exposure time30.0Frames2
Wavelength0.08266Sample-to-detector distance3.17
SourceX-ray synchrotronDetectorPilatus 1M-F
Facility / beamlineTaiwan Photon Source, NSRRC / 23A · Hsinchu, Taiwan
q range0.144 – 2.991Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDC93
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleOligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.
project.publication.titleOligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.
project.publication.author_listShih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US
project.publication.journalPhys Chem Chem Phys
project.publication.doi10.1039/c6cp08820a
project.publication.pmid28262878
project.publication.published_date2017 Mar 15
project.statusreleased
project.submitted_date2017-05-03
project.released_date2017-05-05
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameTaiwan Photon Source, NSRRC
experiment.instrument.cityHsinchu
experiment.instrument.countryTaiwan
experiment.instrument.beamline_name23A
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameApoptosis regulator BAX (Bcl-2 associated X)
experiment.sample.molecule[0].short_nameBAX
experiment.sample.molecule[0].sequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMI AAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ07812
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last192
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS ECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL VLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV LTASLTIWKKMG
experiment.sample.molecule[0].mw21.184
experiment.sample.molecule[0].total_mw84.736
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20mM sodium phosphate 100mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.comment20mM sodium phosphate, 100mM NaCl
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
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experiment.sample.purity_methodnull
experiment.sample.nameTetrameric apoptosis regulator BAX (Bcl-2 associated X)
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experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHsinchu City, Taiwan
experiment.contributor[0].affiliation[0].full_nameNational Synchrotron Radiation Research Center
experiment.contributor[0].affiliation[0].webpagehttps://www.nsrrc.org.tw/
experiment.contributor[0].contributor_nameOrion
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experiment.date2015-05-28
experiment.storage_temperaturenull
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experiment_descriptionSynchrotron SAXS data from solutions of tetrameric apoptosis regulator BAX (Bcl-2 associated X) in 20mM sodium phosphate, 100mM NaCl, pH 8 were collected on the 23A beam line at the Taiwan Photon Source (NSRRC, Hsinchu, Taiwan) using a Pilatus 1M-F detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.08266 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 15°C. Two successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to obtain the SAXS profile displayed in this entry.
tags[]
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symmetrynull
last_modified2021-12-03T19:22:36.894202+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDC93
statussuccess
started_at2026-08-11T13:55:11.624379+00:00
finished_at2026-08-11T13:55:20.614112+00:00
source_last_modified2021-12-03T19:22:36.894202+01:00
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View the complete summary.json source
{
  "code": "SASDC93",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.",
    "publication": {
      "title": "Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.",
      "author_list": "Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US",
      "journal": "Phys Chem Chem Phys",
      "doi": "10.1039/c6cp08820a",
      "pmid": "28262878",
      "published_date": "2017 Mar 15"
    },
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}
View the complete manifest.json source
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