SASDC94

Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

Concentration— – 1.5 Buffer / pH10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA / 7.0
Experimental temperature20.0 Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / G1
Wavelength0.109 nmExposure5.0 s × 50

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Truncated P5abc subdomain from tetrahymena ribozyme
View sequence
GGCAGUACCAAGUCGCGAAAGCGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGCC
RNA———–—monomerMolecule count 118.223 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1276[3]0.02–0.29 1/AHas uncertainty columnMissing 020sasbdb/entries/94/sasdc94/source/SASDC94.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.2—Å
i0Guinier0.650.012—
i0P(r)0.650.004—
mwExperimental30.0—kDa
rgGuinier2.270.05Å
rgP(r)2.320.012Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTABuffer concentration—
pH7.0Additives—
Buffer description0ms time point
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-12-03Storage / measurement temperature4.0 / 20.0
Exposure time5.0Frames50
Wavelength0.109Sample-to-detector distance1.72
SourceX-ray synchrotronDetectorFinger Lakes CCD
Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / G1 · Ithaca, NY, USA
q range0.2 – 2.9Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
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statusPublished
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angular_unit1/A
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project.publication.titleRevealing the distinct folding phases of an RNA three-helix junction.
project.publication.author_listPlumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gky363
project.publication.pmid29762712
project.publication.published_date2018 Aug 21
project.statusreleased
project.submitted_date2017-06-02
project.released_date2018-05-20
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experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameG1
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experiment.sample.molecule[0].long_nameTruncated P5abc subdomain from tetrahymena ribozyme
experiment.sample.molecule[0].short_nametp5abc
experiment.sample.molecule[0].sequenceGGCAGUACCAAGUCGCGAAAGCGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGCC
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeRNA
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experiment.sample.molecule[0].mw18.223
experiment.sample.molecule[0].total_mw18.223
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experiment.contributor[0].affiliation[0].full_nameCornell University
experiment.contributor[0].affiliation[0].webpagehttps://www.cornell.edu/
experiment.contributor[0].contributor_nameAlex
experiment.contributor[0].contributor_surnamePlumridge
experiment.contributor[0].orcidnull
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fits[]
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i0_calibration_standardnull
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experiment_descriptionSynchrotron SAXS data from solutions of Truncated P5abc subdomain from tetrahymena ribozyme (Time-resolved 10ms) in 10mM MOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a CCD Finger Lakes CCD detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 20°C. 50 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
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View the complete manifest.json source
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