SASDC94

Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

浓度— – 1.5 缓冲液 / pH10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA / 7.0
实验温度20.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1
波长0.109 nm曝光5.0 s × 50

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Truncated P5abc subdomain from tetrahymena ribozyme
查看序列
GGCAGUACCAAGUCGCGAAAGCGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGCC
RNA———–—monomer分子数 118.223 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1276[3]0.02–0.29 1/A含误差列缺失 020sasbdb/entries/94/sasdc94/source/SASDC94.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.2—Å
i0Guinier0.650.012—
i0P(r)0.650.004—
mwExperimental30.0—kDa
rgGuinier2.270.05Å
rgP(r)2.320.012Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA缓冲液浓度—
pH7.0添加剂—
缓冲液说明0ms time point
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-12-03储存 / 测量温度4.0 / 20.0
曝光时间5.0帧数50
波长0.109样品-探测器距离1.72
光源X-ray synchrotron探测器Finger Lakes CCD
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1 · Ithaca, NY, USA
q 范围0.2 – 2.9样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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summarysasbdb/entries/94/sasdc94/source/summary.jsondownloaded5961abf53b16af4903082b299a451c1ed4a5fd1baad4ccead01447b65e266633c51e下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
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statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleRevealing the distinct folding phases of an RNA three-helix junction.
project.publication.titleRevealing the distinct folding phases of an RNA three-helix junction.
project.publication.author_listPlumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gky363
project.publication.pmid29762712
project.publication.published_date2018 Aug 21
project.statusreleased
project.submitted_date2017-06-02
project.released_date2018-05-20
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experiment.instrument.detector.resolution0.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameG1
experiment.instrument.beam_geometry
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experiment.sample.molecule[0].short_nametp5abc
experiment.sample.molecule[0].sequenceGGCAGUACCAAGUCGCGAAAGCGAUGGCCUUGCAAAGGGUAUGGUAAUAAGCUGCC
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experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeRNA
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experiment.sample.molecule[0].mw18.223
experiment.sample.molecule[0].total_mw18.223
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experiment.sample.mixturenull
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experiment.contributor[0].affiliation[0].full_nameCornell University
experiment.contributor[0].affiliation[0].webpagehttps://www.cornell.edu/
experiment.contributor[0].contributor_nameAlex
experiment.contributor[0].contributor_surnamePlumridge
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-12-03
experiment.storage_temperature4.0
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fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Truncated P5abc subdomain from tetrahymena ribozyme (Time-resolved 10ms) in 10mM MOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a CCD Finger Lakes CCD detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 20°C. 50 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
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i0_calibration_standard_datanull
intensities_log_log_plotSASDC94_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDC94
statussuccess
started_at2026-08-11T14:16:33.410473+00:00
finished_at2026-08-11T14:16:40.280318+00:00
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查看完整 summary.json 原文
{
  "code": "SASDC94",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Revealing the distinct folding phases of an RNA three-helix junction.",
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      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gky363",
      "pmid": "29762712",
      "published_date": "2018 Aug 21"
    },
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查看完整 manifest.json 原文
{
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  "status": "success",
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