SASDC97

Ethylene Receptor 1 (DHp + CA + RD domains)

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2023-05-25T11:21:52.654103+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Ethylene Receptor 1 (DHp + CA + RD domains)

Concentration0.7 – 5.0 Buffer / pH20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP / 8.8
Experimental temperature10.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength0.154 nmExposure15.0 s × 8

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains
View sequence
TAIRARNDFLAVMNHEMRTPMHAIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPAIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDVCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDNIRDVLSDLLEPRVLYEGM
proteinArabidopsis thalianaP49333339–738dimerMolecule count 244.085 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12151[3]0.138346–6.01554 1/nmHas uncertainty columnMissing 03sasbdb/entries/97/sasdc97/source/SASDC97.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.90.5nm
i0Guinier68.191.0arbitrary
i0P(r)63.69999999975.0arbitrary
mwExperimental90.124.0kDa
mwGuinier I(0)66.45.0kDa
mwPorod90.124.0kDa
porod_volumePorod144.04.0nm³
rgGuinier3.980.1nm
rgP(r)4.10.1nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADPBuffer concentration—
pH8.8Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / 53494Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2010-10-11Storage / measurement temperature10.0 / 10.0
Exposure time15.0Frames8
Wavelength0.154Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 1M-W
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.138 – 6.016Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDC97
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleStructural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
project.publication.titleStructural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
project.publication.author_listMayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M114.587667
project.publication.pmid25451923
project.publication.published_date2015 Jan 30
project.statusreleased
project.submitted_date2017-02-07
project.released_date2017-02-09
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experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEthylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains
experiment.sample.molecule[0].short_nameETR1_DHp-CA-RD
experiment.sample.molecule[0].sequenceTAIRARNDFLAVMNHEMRTPMHAIIALSS LLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLNLIKPIAVVK KLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYL RVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIF DVKLGISERSNESKQSGIPKVPAIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLR VVSHEHKVVFMDVCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVS LDNIRDVLSDLLEPRVLYEGM
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeP49333
experiment.sample.molecule[0].uniprot_range_first339
experiment.sample.molecule[0].uniprot_range_last738
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMEVCNCIEPQWPADELLMKYQYISDFFIAIAYFSIPLELIYFVKKSAVFPYRWVLVQFGA FIVLCGATHLINLWTFTTHSRTVALVMTTAKVLTAVVSCATALMLVHIIPDLLSVKTREL FLKNKAAELDREMGLIRTQEETGRHVRMLTHEIRSTLDRHTILKTTLVELGRTLALEECA LWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG KYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA DQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH AIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH TLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI SVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS SGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA IPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV CMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN IRDVLSDLLEPRVLYEGM
experiment.sample.molecule[0].mw44.085
experiment.sample.molecule[0].total_mw88.17
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEthylene Receptor 1 (DHp + CA + RD domains)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_vol53494
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitnull
experiment.date2010-10-11
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.154
experiment.sample_detector_distance3.1
experiment.concentration_min0.7
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.138
experiment.s_max6.016
experiment.total_exposure_time120.0
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version2.9.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitarbitrary
experimental_mw90.1
experimental_mw_error24.0
guinier_i0_mw66.4
guinier_i0_mw_error5.0
porod_mw90.1
porod_mw_error24.0
pddf_i063.6999999997
pddf_i0_error5.0
guinier_i068.19
guinier_i0_error1.0
pddf_rg4.1
pddf_rg_error0.1
guinier_rg3.98
guinier_rg_error0.1
pddf_dmax13.9
pddf_dmax_error0.5
porod_volume144.0
porod_volume_error4.0
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last71
pddf_point_first30
pddf_point_last727
i0_calibration_standard_datanull
intensities_log_log_plotSASDC97_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDC97
statussuccess
started_at2026-08-11T15:24:25.522712+00:00
finished_at2026-08-11T15:24:33.634740+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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View the complete summary.json source
{
  "code": "SASDC97",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).",
    "publication": {
      "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).",
      "author_list": "Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M114.587667",
      "pmid": "25451923",
      "published_date": "2015 Jan 30"
    },
    "status": "released",
    "submitted_date": "2017-02-07",
    "released_date": "2017-02-09"
  },
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        "resolution": 0.172
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      "city": "Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL X33",
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    "sample": {
      "molecule": [
        {
          "long_name": "Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains",
          "short_name": "ETR1_DHp-CA-RD",
          "sequence": "TAIRARNDFLAVMNHEMRTPMHAIIALSS\r\nLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLNLIKPIAVVK\r\nKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYL\r\nRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIF\r\nDVKLGISERSNESKQSGIPKVPAIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLR\r\nVVSHEHKVVFMDVCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVS\r\nLDNIRDVLSDLLEPRVLYEGM",
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          "uniprot_range_first": 339,
          "uniprot_range_last": 738,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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          "mw": 44.085,
          "total_mw": 88.17,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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        "concentration_unit": null,
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        "concentration": null,
        "pka": null,
        "ph": 8.8,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ethylene Receptor 1 (DHp + CA + RD domains)",
      "ext_coefficient": null,
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      "specific_vol": null,
      "dry_vol": 53494,
      "absorbption": null,
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      "mixture": null
    },
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View the complete manifest.json source
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