SASDCB9

Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2022-08-15T15:12:53.046326+02:00

Additional SANS measurement parameters. Three detector positions (in m) and count times (s) were employed: 1 m (two frames of 600 + 600 = 1200 s total count time); 4 m (two frames of 1800 + 1800 = 3600 s total count time); 13 m (two frames of 600 + 600 = 1200 s total count time).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS

浓度— – 0.88 缓冲液 / pHPBS in D2O / 7.4
实验温度20.0 设备 / 束线NIST Center for High Resolution Neutron Scattering (CHRNS) / NG7
波长0.6 nm曝光1800.0 s × 2

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Apolipoprotein A-I
查看序列
MHHHHHHGENLYFQGMLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTKAQSVIDKASETLTAQ
proteinMus musculusQ0062367–264dimer分子数 225.153 kDa
1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine
查看序列
N/A
other———–—other分子数 —0.732 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1166[3]0.007775–0.5936 1/A含误差列缺失 021sasbdb/entries/b9/sasdcb9/source/SASDCB9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.7—Å
i0Guinier0.0480.00221/cm
i0P(r)0.04670.00131/cm
mwExperimental148.0—kDa
rgGuinier4.3240.767Å
rgP(r)4.2270.069Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称PBS in D2O缓冲液浓度—
pH7.4添加剂—
缓冲液说明8 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, 0.2 g/L KCl, in D2O with no additional pH adjustment
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-04-11储存 / 测量温度4.0 / 20.0
曝光时间1800.0帧数2
波长0.6样品-探测器距离4.0
光源neutron source探测器Ordela 2660N
机构 / 束线NIST Center for High Resolution Neutron Scattering (CHRNS) / NG7 · Gaithersburg, MD, USA
q 范围0.078 – 5.936样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDCB9
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSmall-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.
project.publication.titleSmall-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.
project.publication.author_listCleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.3365
project.publication.pmid29266475
project.publication.published_date2018 Mar
project.statusreleased
project.submitted_date2017-11-21
project.released_date2018-01-09
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experiment.instrument.detector.nameOrdela 2660N
experiment.instrument.detector.resolution5.08
experiment.instrument.nameNIST Center for High Resolution Neutron Scattering (CHRNS)
experiment.instrument.cityGaithersburg, MD
experiment.instrument.countryUSA
experiment.instrument.beamline_nameNG7
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceneutron source
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameApolipoprotein A-I
experiment.sample.molecule[0].short_nameApoA-I
experiment.sample.molecule[0].sequenceMHHHHHHGENLYFQGMLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTKAQSVIDKASETLTAQ
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeQ00623
experiment.sample.molecule[0].uniprot_range_first67
experiment.sample.molecule[0].uniprot_range_last264
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw25.153
experiment.sample.molecule[0].total_mw50.306
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_name1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine
experiment.sample.molecule[1].short_named54-DMPC
experiment.sample.molecule[1].sequenceN/A
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationother
experiment.sample.molecule[1].molecular_typeother
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.732
experiment.sample.molecule[1].total_mw99.0
experiment.sample.molecule[1].number_molecules0
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experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.namePBS in D2O
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.comment8 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, 0.2 g/L KCl, in D2O with no additional pH adjustment
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameNIST
experiment.contributor[0].affiliation[0].addressGaithersburg, MD, USA
experiment.contributor[0].affiliation[0].full_nameNational Institute of Standards and Technology (NIST)
experiment.contributor[0].affiliation[0].webpagehttps://www.nist.gov/
experiment.contributor[0].contributor_nameThomas
experiment.contributor[0].contributor_surnameCleveland
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-04-11
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1800.0
experiment.number_of_frames2
experiment.wavelength0.6
experiment.sample_detector_distance4.0
experiment.concentration_minnull
experiment.concentration_max0.88
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.078
experiment.s_max5.936
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionAdditional SANS measurement parameters. Three detector positions (in m) and count times (s) were employed: 1 m (two frames of 600 + 600 = 1200 s total count time); 4 m (two frames of 1800 + 1800 = 3600 s total count time); 13 m (two frames of 600 + 600 = 1200 s total count time).
experiment_descriptionSmall-angle neutron scattering (SANS) data from solutions of Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC in PBS constituted in 100% D2O, pD 7.4, were collected on the NG7 instrument at the NIST Center for Neutron Research (Gaithersburg, MD, USA) using a 640x640 mm 3He position sensitive Ordela 2660N detector at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.88 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw148.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.0467
pddf_i0_error0.0013
guinier_i00.048
guinier_i0_error0.0022
pddf_rg4.227
pddf_rg_error0.069
guinier_rg4.324
guinier_rg_error0.767
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pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first4
guinier_point_last22
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCB9_datloglog_img.png
symmetrynull
last_modified2022-08-15T15:12:53.046326+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCB9
statussuccess
started_at2026-08-11T16:10:23.093924+00:00
finished_at2026-08-11T16:10:29.491866+00:00
source_last_modified2022-08-15T15:12:53.046326+02:00
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查看完整 summary.json 原文
{
  "code": "SASDCB9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.",
    "publication": {
      "title": "Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.",
      "author_list": "Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P",
      "journal": "Protein Sci",
      "doi": "10.1002/pro.3365",
      "pmid": "29266475",
      "published_date": "2018 Mar"
    },
    "status": "released",
    "submitted_date": "2017-11-21",
    "released_date": "2018-01-09"
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          "oligomerization": "other",
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
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        "name": "PBS in D2O",
        "concentration_unit": null,
        "comment": "8 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, 0.2 g/L KCl, in D2O with no additional pH adjustment",
        "additive": null,
        "concentration": null,
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      "name": "Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS",
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        "affiliation": [
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            "short_name": "NIST",
            "address": "Gaithersburg, MD, USA",
            "full_name": "National Institute of Standards and Technology (NIST)",
            "webpage": "https://www.nist.gov/"
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        "contributor_name": "Thomas",
        "contributor_surname": "Cleveland",
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  "description": "Additional SANS measurement parameters. Three detector positions (in m) and count times (s) were employed: 1 m (two frames of 600 + 600 = 1200 s total count time); 4 m (two frames of 1800 + 1800 = 3600 s total count time); 13 m (two frames of 600 + 600 = 1200 s total count time).",
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCB9_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2022-08-15T15:12:53.046326+02:00",
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}
查看完整 manifest.json 原文
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