Lys63-linked diubiquitin at pH7.4

{
  "code": "SASDCG7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques.",
    "publication": {
      "title": "Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques.",
      "author_list": "Liu Z, Gong Z, Cao Y, Ding YH, Dong MQ, Lu YB, Zhang WP, Tang C",
      "journal": "Biochemistry",
      "doi": "10.1021/acs.biochem.7b00817",
      "pmid": "28945353",
      "published_date": "2018 Jan 23"
    },
    "status": "released",
    "submitted_date": "2017-09-12",
    "released_date": "2017-09-29"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCG7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCG7.dat",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCG7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCG7_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCG7.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "Shanghai Synchrotron Radiation Facility (SSRF)",
      "city": "Shanghai",
      "country": "China",
      "beamline_name": "BL19U2",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Polyubiquitin-C",
          "short_name": "ubiquitin",
          "sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG",
          "organism": "Homo sapiens",
          "uniprot_code": "P0CG48",
          "uniprot_range_first": 305,
          "uniprot_range_last": 379,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN\nIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI\nFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA\nKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT\nITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR\nLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL\nSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ\nQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE\nNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL\nTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH\nLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED\nGRTLSDYNIQKESTLHLVLRLRGGV",
          "mw": 8.565,
          "total_mw": 17.13,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20mM HEPES, 150mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Lys63-linked diubiquitin at pH7.4",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Wuhan, China",
            "full_name": "Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences",
            "webpage": "http://english.wipm.cas.cn/"
          }
        ],
        "contributor_name": "Zhu",
        "contributor_surname": "Liuzhu",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-03-24",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": 1.0,
    "number_of_frames": 20,
    "wavelength": 0.1033,
    "sample_detector_distance": 2.1,
    "concentration_min": null,
    "concentration_max": 2.6,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.197,
    "s_max": 4.611,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Lys63-linked diubiquitin in 20mM HEPES, 150mM NaCl, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facilitystorage ring  (SSRF: Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where  s = 4πsin θ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml (150 µM) was measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 13.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 13.0,
  "porod_mw_error": null,
  "pddf_i0": 66.4,
  "pddf_i0_error": 0.56,
  "guinier_i0": 63.25,
  "guinier_i0_error": 1.64,
  "pddf_rg": 2.1,
  "pddf_rg_error": 0.01,
  "guinier_rg": 2.0,
  "guinier_rg_error": 0.15,
  "pddf_dmax": 7.0,
  "pddf_dmax_error": null,
  "porod_volume": 22.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 4,
  "guinier_point_last": 55,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCG7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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