SASDCL6

Lys63-linked dimer ubiquitin

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Lys63-linked dimer ubiquitin

Concentration— – 5.1 Buffer / pH100mM NaCl, 10mM sodium acetate / 6.0
Experimental temperature25.0 Facility / beamlineShanghai Synchrotron Radiation Facility (SSRF) / BL19U2
Wavelength0.1033 nmExposure1.0 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Polyubiquitin-C
View sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
proteinHomo sapiensP0CG48305–379dimerMolecule count 28.565 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1933[3]0.0123022366–0.469875795 1/AHas uncertainty columnMissing 00sasbdb/entries/l6/sasdcl6/source/SASDCL6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)6.5—Å
i0Guinier69.06640.01—
i0P(r)69.00.07—
mwExperimental14.0—kDa
mwPorod14.0—kDa
porod_volumePorod23.8—ų
rgGuinier2.0840.01Å
rgP(r)2.1150.024Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name100mM NaCl, 10mM sodium acetateBuffer concentration—
pH6.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-03-24Storage / measurement temperature4.0 / 25.0
Exposure time1.0Frames20
Wavelength0.1033Sample-to-detector distance2.126
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineShanghai Synchrotron Radiation Facility (SSRF) / BL19U2 · Shanghai, China
q range0.123 – 4.699Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDCL6
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleLys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
project.publication.titleLys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
project.publication.author_listLiu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
project.publication.journalElife
project.publication.doi10.7554/eLife.05767
project.publication.pmid26090905
project.publication.published_date2015 Jun 19
project.statusreleased
project.submitted_date2017-07-18
project.released_date2017-07-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDCL6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDCL6.dat
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDCL6.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameShanghai Synchrotron Radiation Facility (SSRF)
experiment.instrument.cityShanghai
experiment.instrument.countryChina
experiment.instrument.beamline_nameBL19U2
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePolyubiquitin-C
experiment.sample.molecule[0].short_nameubiquitin
experiment.sample.molecule[0].sequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP0CG48
experiment.sample.molecule[0].uniprot_range_first305
experiment.sample.molecule[0].uniprot_range_last379
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT ITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR LRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL SDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL TGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH LVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED GRTLSDYNIQKESTLHLVLRLRGGV
experiment.sample.molecule[0].mw8.565
experiment.sample.molecule[0].total_mw17.13
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name100mM NaCl, 10mM sodium acetate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLys63-linked dimer ubiquitin
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressWuhan, China
experiment.contributor[0].affiliation[0].full_nameWuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences
experiment.contributor[0].affiliation[0].webpagehttp://english.wipm.cas.cn/
experiment.contributor[0].contributor_nameZhu
experiment.contributor[0].contributor_surnameLiuzhu
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-03-24
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time1.0
experiment.number_of_frames20
experiment.wavelength0.1033
experiment.sample_detector_distance2.126
experiment.concentration_minnull
experiment.concentration_max5.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.123
experiment.s_max4.699
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.
tags[]
intensity_unitnull
experimental_mw14.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw14.0
porod_mw_errornull
pddf_i069.0
pddf_i0_error0.07
guinier_i069.0664
guinier_i0_error0.01
pddf_rg2.115
pddf_rg_error0.024
guinier_rg2.084
guinier_rg_error0.01
pddf_dmax6.5
pddf_dmax_errornull
porod_volume23.8
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first31
guinier_point_last101
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCL6_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDCL6
statussuccess
started_at2026-08-11T15:11:44.330977+00:00
finished_at2026-08-11T15:11:52.081376+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDCL6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.",
    "publication": {
      "title": "Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.",
      "author_list": "Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C",
      "journal": "Elife",
      "doi": "10.7554/eLife.05767",
      "pmid": "26090905",
      "published_date": "2015 Jun 19"
    },
    "status": "released",
    "submitted_date": "2017-07-18",
    "released_date": "2017-07-18"
  },
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      "country": "China",
      "beamline_name": "BL19U2",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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          "long_name": "Polyubiquitin-C",
          "short_name": "ubiquitin",
          "sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG",
          "organism": "Homo sapiens",
          "uniprot_code": "P0CG48",
          "uniprot_range_first": 305,
          "uniprot_range_last": 379,
          "oligomerization": "dimer",
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  "tags": [],
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  "experimental_mw_error": null,
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View the complete manifest.json source
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