2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
dmax
P(r)
6.5
—
Å
i0
Guinier
69.0664
0.01
—
i0
P(r)
69.0
0.07
—
mw
Experimental
14.0
—
kDa
mw
Porod
14.0
—
kDa
porod_volume
Porod
23.8
—
ų
rg
Guinier
2.084
0.01
Å
rg
P(r)
2.115
0.024
Å
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
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3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
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4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/l6/sasdcl6/source/SASDCL6.dat
downloaded
43881
full_entry_zip
sasbdb/entries/l6/sasdcl6/source/SASDCL6.zip
downloaded
28292
pddf
sasbdb/entries/l6/sasdcl6/source/SASDCL6.out
downloaded
43605
sascif
sasbdb/entries/l6/sasdcl6/source/SASDCL6.sascif
downloaded
83750
summary
sasbdb/entries/l6/sasdcl6/source/summary.json
downloaded
6665
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5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.
Related Publication
Title
Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
Authors
Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
DOI / PMID
10.7554/eLife.05767 / 26090905
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences
experiment.contributor[0].affiliation[0].webpage
http://english.wipm.cas.cn/
experiment.contributor[0].contributor_name
Zhu
experiment.contributor[0].contributor_surname
Liuzhu
experiment.contributor[0].orcid
null
experiment.concentration_method
null
experiment.concentration_unit
null
experiment.date
2016-03-24
experiment.storage_temperature
4.0
experiment.cell_temperature
25.0
experiment.exposure_time
1.0
experiment.number_of_frames
20
experiment.wavelength
0.1033
experiment.sample_detector_distance
2.126
experiment.concentration_min
null
experiment.concentration_max
5.1
experiment.sample_volume
null
experiment.flow_rate
null
experiment.s_min
0.123
experiment.s_max
4.699
experiment.total_exposure_time
null
experiment.seccolumn
null
fits
[]
estimated_volume_method
null
pddf_software
ATSAS GNOM
pddf_software_version
null
i0_calibration_standard
null
description
null
experiment_description
Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.