Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

{
  "code": "SASDCP5",
  "status": "Published",
  "type_of_curve": "Other",
  "angular_unit": "1/nm",
  "project": {
    "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
    "publication": {
      "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
      "author_list": "Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-01542-z",
      "pmid": "29129932",
      "published_date": "2017 Nov 13"
    },
    "status": "released",
    "submitted_date": "2017-06-27",
    "released_date": "2017-11-20"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCP5.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCP5.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP5_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCP5_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCP5.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "DNA polymerase processivity factor component A20 C-ter fragment",
          "short_name": "A20 C-ter",
          "sequence": "GNGKYFSKVGSAGLKQLTNKLDINECATVDELVDEINKSGTVKRKIKNQSAFDLSRECLGYPEADFITLVNNMRFKIENC\r\nKVVNFNIENTNCLNNPSIETIYRNFNQFVSIFNVVTDVKKRLFENASGNGSGGGLNDIFEAQKIEWHE",
          "organism": "Vaccinia virus",
          "uniprot_code": "P20995",
          "uniprot_range_first": 304,
          "uniprot_range_last": 426,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTSSADLTNLKELLSLYKSLRFSDSAAIEKYNSLVEWGTSTYWKIGVQKVANVETSISDY\nYDEVKNKPFNIDPGYYIFLPVYFGSVFIYSKGKNMVELGSGNSFQIPDDMRSACNKVLDS\nDNGIDFLRFVLLNNRWIMEDAISKYQSPVNIFKLASEYGLNIPKYLEIEIEEDTLFDDEL\nYSIIERSFDDKFPKISISYIKLGELRRQVVDFFKFSFMYIESIKVDRIGDNIFIPSVITK\nSGKKILVKDVDHLIRSKVREHTFVKVKKKNTFSILYDYDGNGTETRGEVIKRIIDTIGRD\nYYVNGKYFSKVGSAGLKQLTNKLDINECATVDELVDEINKSGTVKRKIKNQSAFDLSREC\nLGYPEADFITLVNNMRFKIENCKVVNFNIENTNCLNNPSIETIYRNFNQFVSIFNVVTDV\nKKRLFE",
          "mw": 16.65,
          "total_mw": 16.65,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Tris-HCl, 300 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "IBS",
            "address": "Grenoble, France",
            "full_name": "Institut de Biologie Structurale",
            "webpage": ""
          }
        ],
        "contributor_name": "Wim",
        "contributor_surname": "Burmeister",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-02-05",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 3000,
    "wavelength": 0.0931,
    "sample_detector_distance": 2.34,
    "concentration_min": null,
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    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.033,
    "s_max": 4.945,
    "total_exposure_time": null,
    "seccolumn": null
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          "bead_radius": 1.6,
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          "symmetry": "none",
          "comment": "best model of 40",
          "user": 226
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCP5_fit1_fit_img.png",
      "software": "DAMMIF",
      "chi_square_value": 0.254,
      "p_value": 0.000148,
      "fit_residual_plot": "SASDCP5_fit1_fitresiduals_img.png",
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of A20 C-ter fragment in 25 mM Tris-HCl, 300 mM NaCl, pH 7.5 were collected using size exclusion chromatography coupled SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.0931 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were collected at at 20°C during the course of elution (3000 successive 1 second frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the resulting subtracted curves were scaled and averaged.",
  "tags": [],
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  "experimental_mw": 19.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 19.0,
  "porod_mw_error": null,
  "pddf_i0": 5.58,
  "pddf_i0_error": 0.01,
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  "guinier_i0_error": 0.01,
  "pddf_rg": 2.15,
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  "guinier_rg_error": 0.01,
  "pddf_dmax": 7.4,
  "pddf_dmax_error": null,
  "porod_volume": 31.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 18,
  "guinier_point_last": 88,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCP5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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