SASDCP8

Ras-related protein Rab-1A

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2024-10-31T15:53:47.550615+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Ras-related protein Rab-1A

Concentration2.0 – 6.0 Buffer / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
Experimental temperature15.0 Facility / beamlineSOLEIL / SWING
Wavelength0.103 nmExposure1.2 s × 240

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Ras-related protein Rab-1A
View sequence
MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCCHHHHHH
proteinHomo sapiensP62820—–—monomerMolecule count 123.5 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11062[3]0.01690595–0.6124736 1/AHas uncertainty columnMissing 013sasbdb/entries/p8/sasdcp8/source/SASDCP8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.2—Å
i0Guinier0.013661.3e-051/cm
i0P(r)0.013851.4e-051/cm
mwExperimental24.0—kDa
mwPorod24.0—kDa
porod_volumePorod36.1—ų
rgGuinier2.380.004Å
rgP(r)2.540.006Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-11-17Storage / measurement temperature— / 15.0
Exposure time1.2Frames240
Wavelength0.103Sample-to-detector distance1.2
SourceX-ray synchrotronDetectorAVIEX PCCD170170
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.169 – 6.125Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDCP8
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleThe SAXS Analysis of the human GTPase Rab1a
project.publicationnull
project.statusreleased
project.submitted_date2017-10-29
project.released_date2024-10-29
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDCP8.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDCP8.dat
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP8_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDCP8.sascif
experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameRas-related protein Rab-1A
experiment.sample.molecule[0].short_nameRab1a
experiment.sample.molecule[0].sequenceMSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQ ERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAK EFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCCHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP62820
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTI KLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKL LVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGA TAGGAEKSNVKIQSTPVKQSGGGCC
experiment.sample.molecule[0].mw23.5
experiment.sample.molecule[0].total_mw23.5
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameRas-related protein Rab-1A
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-17
experiment.storage_temperaturenull
experiment.cell_temperature15.0
experiment.exposure_time1.2
experiment.number_of_frames240
experiment.wavelength0.103
experiment.sample_detector_distance1.2
experiment.concentration_min2.0
experiment.concentration_max6.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.169
experiment.s_max6.125
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
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fits[0].models[0].model_mw24.2
fits[0].models[0].bead_radius1.4
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/Rab1-df.log
fits[0].models[0].symmetryP1
fits[0].models[0].comment
fits[0].models[0].user84
fits[0].fit_unit1/A
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fits[0].softwareDAMMIF
fits[0].chi_square_value1.745
fits[0].p_value5e-06
fits[0].fit_residual_plotSASDCP8_fit1_fitresiduals_img.png
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fits[0].software_version2.8.2
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version2.8.2
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Ras-related protein Rab-1A in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5, were collected at the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 2 and 6 mg/ml were measured at 15°C. 240 successive 1.2second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
tags[]
intensity_unit1/cm
experimental_mw24.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw24.0
porod_mw_errornull
pddf_i00.01385
pddf_i0_error1.4e-05
guinier_i00.01366
guinier_i0_error1.3e-05
pddf_rg2.54
pddf_rg_error0.006
guinier_rg2.38
guinier_rg_error0.004
pddf_dmax10.2
pddf_dmax_errornull
porod_volume36.1
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first11
guinier_point_last59
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCP8_datloglog_img.png
symmetrynull
last_modified2024-10-31T15:53:47.550615+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDCP8
statussuccess
started_at2026-08-11T15:57:42.652391+00:00
finished_at2026-08-11T15:57:51.249364+00:00
source_last_modified2024-10-31T15:53:47.550615+01:00
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View the complete summary.json source
{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "The SAXS Analysis of the human GTPase Rab1a",
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  },
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View the complete manifest.json source
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