SASDCU5

SAXS dilution series on ground Doryteuthis squid lens 100% layer

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2022-01-27T08:12:51.670715+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · SAXS dilution series on ground Doryteuthis squid lens 100% layer

浓度— – — 18缓冲液 / pHPBS, phosphate buffered saline / 7.0
实验温度— 设备 / 束线National Synchrotron Light Source (NSLS) / X9A
波长0.1 nm曝光— s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
S-crystallin, Q25367_DOROP
查看序列
MPTYTLYYFNGRGRAEVLRMMFAAANVKYMDKRFEFNEWDKYRKDMPSMCVPVLEMDGGNKMPETMAIPRYLAREFGFYGKSNMDMMRCDYIADCFYEIMHDYMRYFHEKNGRFRSSMEGSGNGMNSPTGASGDMNSNSNFDTYLQWRFMNTCNRVLPFLERTLEMQNGGKAFFMGDQMMWCDMMCHCALEGPFMENQSMLSKHPKLMALRTRIASHPKISGYLKSRSNTNW
proteinDoryteuthis pealeiiQ253671–232dimer分子数 227.289 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1222[3]0.008–1.72 1/A含误差列缺失 00sasbdb/entries/u5/sasdcu5/source/SASDCU5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
i0Guinier794.0——
mwExperimental55.0—kDa
rgGuinier2.4—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称PBS, phosphate buffered saline缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-11-12储存 / 测量温度— / —
曝光时间—帧数—
波长0.1样品-探测器距离—
光源X-ray synchrotron探测器Pilatus 300K
机构 / 束线National Synchrotron Light Source (NSLS) / X9A · Brookhaven, NY, USA
q 范围0.08 – 17.2样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/u5/sasdcu5/source/SASDCU5.outnot_listed——下载查看原文件
sascifsasbdb/entries/u5/sasdcu5/source/SASDCU5.sascifdownloaded17457d09e82702214061ec0a09aeefeb8ee3a127151633f2d3ab2d1c04c466ca29930下载查看原文件源站
summarysasbdb/entries/u5/sasdcu5/source/summary.jsondownloaded6859c492c2bc14a1f99bd30b93bafa2e426d2546f7baa5ea786f8ad14465faad6bad下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(3 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDCU5
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleEye patches: Protein assembly of index-gradient squid lenses.
project.publication.titleEye patches: Protein assembly of index-gradient squid lenses.
project.publication.author_listCai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM
project.publication.journalScience
project.publication.doi10.1126/science.aal2674
project.publication.pmid28798124
project.publication.published_date2017 Aug 11
project.statusreleased
project.submitted_date2017-06-29
project.released_date2017-08-18
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameNational Synchrotron Light Source (NSLS)
experiment.instrument.cityBrookhaven, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameX9A
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
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experiment.sample.molecule[0].long_nameS-crystallin, Q25367_DOROP
experiment.sample.molecule[0].short_nameS-crystallin, Q25367
experiment.sample.molecule[0].sequenceMPTYTLYYFNGRGRAEVLRMMFAAANVKYMDKRFEFNEWDKYRKDMPSMCVPVLEMDGGN KMPETMAIPRYLAREFGFYGKSNMDMMRCDYIADCFYEIMHDYMRYFHEKNGRFRSSMEG SGNGMNSPTGASGDMNSNSNFDTYLQWRFMNTCNRVLPFLERTLEMQNGGKAFFMGDQMM WCDMMCHCALEGPFMENQSMLSKHPKLMALRTRIASHPKISGYLKSRSNTNW
experiment.sample.molecule[0].organismDoryteuthis pealeii
experiment.sample.molecule[0].uniprot_codeQ25367
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last232
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPTYTLYYFNGRGRAEVLRMMFAAANVKYMDKRFEFNEWDKYRKDMPSMCVPVLEMDGGN KMPETMAIPRYLAREFGFYGKSNMDMMRCDYIADCFYEIMHDYMRYFHEKNGRFRSSMEG SGNGMNSPTGASGDMNSNSNFDTYLQWRFMNTCNRVLPFLERTLEMQNGGKAFFMGDQMM WCDMMCHCALEGPFMENQSMLSKHPKLMALRTRIASHPKISGYLKSRSNTNW
experiment.sample.molecule[0].mw27.289
experiment.sample.molecule[0].total_mw54.578
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.namePBS, phosphate buffered saline
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSAXS dilution series on ground Doryteuthis squid lens 100% layer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameJing
experiment.contributor[0].contributor_surnameCai
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unit18
experiment.date2013-11-12
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experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of ground Doryteuthis squid lens (100% layer) diluted in phosphate buffered saline, pH 7, were collected on the X9A camera on the storage ring National Synchrotron Light Source (NSLS; Brookhaven, NY, USA) using a 20Hz Pilatus 300K detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged, and the scattering of the solvent blank (PBS) was subtracted from the diluted lens data. The dataset shown in this entry was collected from a suspension containing about 18 mg/ml of lens protein (refer to the full entry zip archive for the complete dilution series of 9, 4.5, 2.25, 1.13, 0.56, 0.28, 0.14 and 0.07 mg/ml lens protein). The lens contains about 40 unique S-crystallins isoforms that vary in molecular weight due to length variation in a disordered region encoded in the central exon of the coding sequence such that loops form protruding from the folded protein. S-crystallins are dimeric and globular, such that each dimer has two of these disordered loops, which form linking structures between particles in the system. The full set of sequences describing the protein polydispersity in this system is available at GenBank BioProject PRJNA386124.
tags[0]softmatter
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experimental_mw55.0
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pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCU5_datloglog_img.png
symmetrynull
last_modified2022-01-27T08:12:51.670715+01:00
bragg_peak[]
manifest.json:33 个字段值
字段路径原始值
codeSASDCU5
statussuccess
started_at2026-08-11T14:56:19.214185+00:00
finished_at2026-08-11T14:56:24.620109+00:00
source_last_modified2022-01-27T08:12:51.670715+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCU5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Eye patches: Protein assembly of index-gradient squid lenses.",
    "publication": {
      "title": "Eye patches: Protein assembly of index-gradient squid lenses.",
      "author_list": "Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM",
      "journal": "Science",
      "doi": "10.1126/science.aal2674",
      "pmid": "28798124",
      "published_date": "2017 Aug 11"
    },
    "status": "released",
    "submitted_date": "2017-06-29",
    "released_date": "2017-08-18"
  },
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  "guinier_i0": 794.0,
  "guinier_i0_error": null,
  "pddf_rg": null,
  "pddf_rg_error": null,
  "guinier_rg": 2.4,
  "guinier_rg_error": null,
  "pddf_dmax": null,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": null,
  "guinier_point_last": null,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCU5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2022-01-27T08:12:51.670715+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
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  "status": "success",
  "started_at": "2026-08-11T14:56:19.214185+00:00",
  "finished_at": "2026-08-11T14:56:24.620109+00:00",
  "source_last_modified": "2022-01-27T08:12:51.670715+01:00",
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      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDCU5.zip",
      "path": "/data/saxsdb/sasbdb/entries/u5/sasdcu5/source/SASDCU5.zip",
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}