SASDD22

Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2020-08-28T15:27:27.968395+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

Concentration— – 16.0 Buffer / pH30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine / 7.9
Experimental temperature20.0 Facility / beamlineSOLEIL / SWING
Wavelength0.1033 nmExposure1.0 s × 250

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Calvin cycle protein CP12, chloroplastic
View sequence
HHHHHHHHHHSSGHIEGRHMSGQPAVDLNKKVQDAVKEAEDACAKGTSADCAVAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED
proteinChlamydomonas reinhardtiiA6Q0K528–107monomerMolecule count 110.934 kDa
Glyceraldehyde-3-phosphate dehydrogenase
View sequence
EKKIRVAINGFGRIGRNFLRCWHGRQNTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQLPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKHEYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFREAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQRVVDLAEVTAKKWVA
proteinChlamydomonas reinhardtiiA8HP84—–—tetramerMolecule count 436.881 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1813[3]0.01052867–0.4595047 1/AHas uncertainty columnMissing 00sasbdb/entries/22/sasdd22/source/SASDD22.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)19.01.0Å
i0Guinier0.00038521e-081/cm
i0P(r)0.00038772e-071/cm
mwExperimental166.0—kDa
porod_volumePorod250.82—ų
rgGuinier3.840.01Å
rgP(r)3.990.006Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteineBuffer concentration—
pH7.9Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2010-12-08Storage / measurement temperature15.0 / 20.0
Exposure time1.0Frames250
Wavelength0.1033Sample-to-detector distance1.832
SourceX-ray synchrotronDetectorAVIEX PCCD170170
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.105 – 4.595Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (6 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:172 field values
Field pathRaw value
codeSASDD22
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleCryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.
project.publication.titleCryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.
project.publication.author_listLaunay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
project.publication.journalJ Mol Biol
project.publication.doi10.1016/j.jmb.2018.02.020
project.publication.pmid29501381
project.publication.published_date2018 Apr 13
project.statusreleased
project.submitted_date2017-12-04
project.released_date2018-03-11
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDD22.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDD22.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_dat_img.png
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDD22.sascif
experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameCalvin cycle protein CP12, chloroplastic
experiment.sample.molecule[0].short_nameCP12
experiment.sample.molecule[0].sequenceHHHHHHHHHHSSGHIEGRHMSGQPAVDLNKKVQDAVKEAEDACAKGTSADCAVAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED
experiment.sample.molecule[0].organismChlamydomonas reinhardtii
experiment.sample.molecule[0].uniprot_codeA6Q0K5
experiment.sample.molecule[0].uniprot_range_first28
experiment.sample.molecule[0].uniprot_range_last107
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMMLTKSVVISRPAVRPVSTRRAVVVRASGQPAVDLNKKVQDAVKEAEDACAKGTSADCAV AWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED
experiment.sample.molecule[0].mw10.934
experiment.sample.molecule[0].total_mw10.934
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameGlyceraldehyde-3-phosphate dehydrogenase
experiment.sample.molecule[1].short_nameGAPDH
experiment.sample.molecule[1].sequenceEKKIRVAINGFGRIGRNFLRCWHGRQNTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQLPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKHEYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFREAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQRVVDLAEVTAKKWVA
experiment.sample.molecule[1].organismChlamydomonas reinhardtii
experiment.sample.molecule[1].uniprot_codeA8HP84
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationtetramer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMAAMMQKSAFTGSAVSSKSGVRAKAARAVVDVRAEKKIRVAINGFGRIGRNFLRCWHGRQ NTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQ LPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKH EYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAA LNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFR EAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQR VVDLAEVTAKKWVA
experiment.sample.molecule[1].mw36.881
experiment.sample.molecule[1].total_mw147.524
experiment.sample.molecule[1].number_molecules4
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.9
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameGlyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMarseille, France
experiment.contributor[0].affiliation[0].full_nameAix Marseille Univ, CNRS, BIP UMR7281
experiment.contributor[0].affiliation[0].webpagehttp://bip.cnrs-mrs.fr
experiment.contributor[0].contributor_nameVeronique
experiment.contributor[0].contributor_surnameReceveur-Brechot
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2010-12-08
experiment.storage_temperature15.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames250
experiment.wavelength0.1033
experiment.sample_detector_distance1.832
experiment.concentration_minnull
experiment.concentration_max16.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.105
experiment.s_max4.595
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[]
fits[0].fit_unit1/A
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fits[0].softwareEOM/GAJOE
fits[0].chi_square_value5.291
fits[0].p_value0.0
fits[0].fit_residual_plotfit4columns1223_fitresiduals_img.png
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fits[0].descriptionnull
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fits[1].softwareEOM/GAJOE
fits[1].chi_square_value29.721
fits[1].p_value0.0
fits[1].fit_residual_plotfit4columns1224_fitresiduals_img.png
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fits[1].software_versionnull
fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 in 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH 7.9 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 16.00 mg/ml was measured at 20°C. 250 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
tags[]
intensity_unit1/cm
experimental_mw166.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.0003877
pddf_i0_error2e-07
guinier_i00.0003852
guinier_i0_error1e-08
pddf_rg3.99
pddf_rg_error0.006
guinier_rg3.84
guinier_rg_error0.01
pddf_dmax19.0
pddf_dmax_error1.0
porod_volume250.82
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last31
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDD22_datloglog_img.png
symmetrynull
last_modified2020-08-28T15:27:27.968395+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDD22
statussuccess
started_at2026-08-11T13:28:49.310425+00:00
finished_at2026-08-11T13:28:57.853621+00:00
source_last_modified2020-08-28T15:27:27.968395+02:00
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View the complete summary.json source
{
  "code": "SASDD22",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.",
    "publication": {
      "title": "Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.",
      "author_list": "Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2018.02.020",
      "pmid": "29501381",
      "published_date": "2018 Apr 13"
    },
    "status": "released",
    "submitted_date": "2017-12-04",
    "released_date": "2018-03-11"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD22.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD22.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_dat_img.png",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD22_pofr_img.png",
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        "resolution": null
      },
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      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Calvin cycle protein CP12, chloroplastic",
          "short_name": "CP12",
          "sequence": "HHHHHHHHHHSSGHIEGRHMSGQPAVDLNKKVQDAVKEAEDACAKGTSADCAVAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED",
          "organism": "Chlamydomonas reinhardtii",
          "uniprot_code": "A6Q0K5",
          "uniprot_range_first": 28,
          "uniprot_range_last": 107,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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            "webpage": "http://bip.cnrs-mrs.fr"
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View the complete manifest.json source
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