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{
"code": "SASDD52",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP.",
"publication": {
"title": "Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP.",
"author_list": "Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN",
"journal": "Biochim Biophys Acta",
"doi": "10.1016/j.bbabio.2018.03.001",
"pmid": "29524381",
"published_date": "2018 May"
},
"status": "released",
"submitted_date": null,
"released_date": "2018-03-10"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD52.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD52.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD52_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDD52.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Pilatus 2M",
"resolution": null
},
"name": "PETRA III",
"city": "DESY; Hamburg",
"country": "Germany",
"beamline_name": "EMBL P12",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Uncharacterized fluorescence recovery protein",
"short_name": "FRP",
"sequence": "MSEINWSEQDKNAAVDALHKAYEREVKILIDEVKQKAFNLTEIDEVWKLHDFLSARRHDI\r\nDGKYDYRDAASVFVLATLVKQGWLSLEELQVLGQDKLAKISALTRL",
"organism": "Arthrospira maxima CS-328",
"uniprot_code": "B5W3T4",
"uniprot_range_first": 1,
"uniprot_range_last": 106,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MSEINWSEQDKNAAVDALHKAYEREVKILIDEVKQKAFNLTEIDEVWKLHDFLSARRHDI\nDGKYDYRDAASVFVLATLVKQGWLSLEELQVLGQDKLAKISALTRL",
"mw": 12.244,
"total_mw": 24.488,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.6,
"deuteration": null
},
"purity_method": null,
"name": "Arthrospira fluorescence recovery protein AmaxFRP.1-106",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": "EMBL-Hamburg",
"address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
"full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
"webpage": "http://www.embl-hamburg.de/index.php"
}
],
"contributor_name": "Cy M",
"contributor_surname": "Jeffries",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2017-09-01",
"storage_temperature": 10.0,
"cell_temperature": 10.0,
"exposure_time": 0.05,
"number_of_frames": 20,
"wavelength": 0.124,
"sample_detector_distance": null,
"concentration_min": null,
"concentration_max": 5.77,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.088,
"s_max": 3.886,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDD52_fit1_model1_img.png",
"software": "GASBOR",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": "2.3i",
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDD52_fit1_model1.pdb",
"model_mw": null,
"bead_radius": 1.9,
"log": null,
"symmetry": "P2",
"comment": null,
"user": 9
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD52_fit1_fit_img.png",
"software": "GASBOR",
"chi_square_value": 1.08,
"p_value": 0.48876,
"fit_residual_plot": "SASDD52_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD52_fit1.fir",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Amax4.log",
"software_version": "2.3i",
"description": null
},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDD52_fit2_model1_img.png",
"software": "iTASSER",
"pdb_link": [],
"model_title": null,
"type_of_model": "atomic",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDD52_fit2_model1.pdb",
"model_mw": 24.44,
"bead_radius": null,
"log": null,
"symmetry": "P2",
"comment": "Fitted using CRYSOL with 25 harmonics, 25 points and constant adjusment.",
"user": 9
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD52_fit2_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.127,
"p_value": 0.285306,
"fit_residual_plot": "SASDD52_fit2_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD52_fit2.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/AmaxFRP00.log",
"software_version": "2.8.3",
"description": null
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": "Additional GASBOR models, with spatial alignments and the NSD estimate can be located in the full entry zip archive.",
"experiment_description": "Synchrotron SAXS data from solutions of Arthrospira fluorescence recovery protein AmaxFRP.1-106 in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.77 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.",
"tags": [],
"intensity_unit": "1/cm",
"experimental_mw": 22.0,
"experimental_mw_error": null,
"guinier_i0_mw": 22.0,
"guinier_i0_mw_error": null,
"porod_mw": 22.0,
"porod_mw_error": null,
"pddf_i0": 0.01621,
"pddf_i0_error": null,
"guinier_i0": 0.0161,
"guinier_i0_error": 3e-05,
"pddf_rg": 2.786,
"pddf_rg_error": null,
"guinier_rg": 2.68,
"guinier_rg_error": 0.02,
"pddf_dmax": 9.5,
"pddf_dmax_error": null,
"porod_volume": 35.0,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 1,
"guinier_point_last": 144,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDD52_datloglog_img.png",
"symmetry": null,
"last_modified": "2023-06-21T07:56:39.492710+02:00",
"bragg_peak": []
}
