SASDDB8

Periplasmic domain of inner membrane protein GspL, dimer

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Periplasmic domain of inner membrane protein GspL, dimer

Concentration2.0 – 13.7 Buffer / pH50 mM TRIS, 100 mM NaCl / 7.5
Experimental temperature15.0 Facility / beamlineSOLEIL / SWING
Wavelength0.1033 nmExposure— s × 19

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Type II secretion system protein L, periplasmic domain
View sequence
WSHPQFEKGGGDRYAAQSAELYRQLFPEDRKLINLRAQFDQHLADSASSGGEGQLLGLLGQAATVIGGEPTVSVEQLDFSAARGDVALQVRAPGFDVLERLRSRLSESGLAVQLGSASRDGSTVSARLVIGG
proteinPseudomonas aeruginosaP25060261–382dimerMolecule count 213.966 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11086[4]0.00795134474354–0.621731749979 1/AHas uncertainty columnMissing 04sasbdb/entries/b8/sasddb8/source/SASDDB8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.5—Å
i0Guinier0.03913392.5e-05arbitrary
i0P(r)0.03865—arbitrary
mwExperimental22.8—kDa
mwGuinier I(0)36.4—kDa
rgGuinier2.2430.07Å
rgP(r)2.21—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM TRIS, 100 mM NaClBuffer concentration—
pH7.5Additives—
Buffer descriptionpH=7.5 at 25°C
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-04-08Storage / measurement temperature4.0 / 15.0
Exposure time—Frames19
Wavelength0.1033Sample-to-detector distance—
SourceX-ray synchrotronDetectorAVIEX PCCD170170
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.08 – 6.139Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:221 field values
Field pathRaw value
codeSASDDB8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.author_listFulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN
project.publication.journalSci Rep
project.publication.doi10.1038/s41598-018-34956-w
project.publication.pmid30425318
project.publication.published_date2018 Nov 13
project.statusreleased
project.submitted_date2018-06-11
project.released_date2018-11-13
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experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameType II secretion system protein L, periplasmic domain
experiment.sample.molecule[0].short_nameGspLperi
experiment.sample.molecule[0].sequenceWSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD QHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS AARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD GSTVSARLVI GG
experiment.sample.molecule[0].organismPseudomonas aeruginosa
experiment.sample.molecule[0].uniprot_codeP25060
experiment.sample.molecule[0].uniprot_range_first261
experiment.sample.molecule[0].uniprot_range_last382
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSGVSALFLPPASTAGADGELAVWWVQDGECRRAPFAQALAEIRAPWRLYLPVEAVTACA VNLPTQKARWLRQSLPFAVEEQLADDVEQMHLALGPALADGRHRVFAVQRTWLAAWLALA EGAGKAPASLHVDADCLPGEGSCLFWLEERWLLGGSGAVRLACGSEDWPVLRDSCPPPQR AFAAQEVAPLEGVEVQALAGNPHVWLSEQPLGTDLAQAEFAARQQSSQWRRWRPLLGLVG LWLVLQWGFTLVQAWQLQREGDRYAAQSAELYRQLFPEDRKLINLRAQFDQHLADSASSG GEGQLLGLLGQAATVIGGEPTVSVEQLDFSAARGDVALQVRAPGFDVLERLRSRLSESGL AVQLGSASRDGSTVSARLVIGG
experiment.sample.molecule[0].mw13.966
experiment.sample.molecule[0].total_mw27.932
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM TRIS, 100 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentpH=7.5 at 25°C
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePeriplasmic domain of inner membrane protein GspL, dimer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGent, Belgium
experiment.contributor[0].affiliation[0].full_nameGhent University
experiment.contributor[0].affiliation[0].webpagehttps://www.ugent.be/
experiment.contributor[0].contributor_nameAleksandra
experiment.contributor[0].contributor_surnameFulara
experiment.contributor[0].orcid
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experiment.concentration_unitnull
experiment.date2016-04-08
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_timenull
experiment.number_of_frames19
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tags[]
intensity_unitarbitrary
experimental_mw22.8
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDB8
statussuccess
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View the complete summary.json source
{
  "code": "SASDDB8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
    "publication": {
      "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
      "author_list": "Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN",
      "journal": "Sci Rep",
      "doi": "10.1038/s41598-018-34956-w",
      "pmid": "30425318",
      "published_date": "2018 Nov 13"
    },
    "status": "released",
    "submitted_date": "2018-06-11",
    "released_date": "2018-11-13"
  },
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