SASDDE7

Class I chitinase 1 from Agave tequilana

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Class I chitinase 1 from Agave tequilana

Concentration— – 0.23 Buffer / pHMES 50 mM / 6.0
Experimental temperature15.0 Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
Wavelength1.127 nmExposure1.0 s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Chitinase 1
View sequence
GGAQQCGSQAGGAVCPNGLCCSQFGYCGSTSPYCGNGCQSQCGGGSSPTPNPPSGGGGGGGGGGSGVGSIISSSLFDQILLHRNDAACPAHGFYTYDAFVAAANAFSGFATTGDADTQKREIAAFLAQTSHETTGGWPTAPDGPYSWGYCFLQEQGNPGDYCVPNDQWPCAPGKKYYGRGPIQISYNYNYGPCGNAIGSDLLNNPDLVASDPTVSFKTALWFWMTPQSPKPSCHDVITGQWTPSAADQAAGRVPGFGVITNIINGGVECGHGSDSRDEDRVGFYKRYCDILGVSYGDNLDCGNQSHF
proteinAgave tequilana——–—monomerMolecule count 131.753 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1444[3]0.0131603–0.418793 1/AHas uncertainty columnMissing 05sasbdb/entries/e7/sasdde7/source/SASDDE7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.66—Å
i0Guinier257.541.72—
i0P(r)257.2——
mwExperimental31.7—kDa
mwGuinier I(0)30.52.0kDa
mwPorod32.5—kDa
porod_volumePorod52.0—ų
rgGuinier2.5510.029Å
rgP(r)2.61—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer nameMES 50 mMBuffer concentration—
pH6.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-04-26Storage / measurement temperature4.0 / 15.0
Exposure time1.0Frames—
Wavelength1.127Sample-to-detector distance1.78
SourceX-ray synchrotronDetectorRayonix MX225-HE
Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q range0.132 – 4.188Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDDE7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.author_listSierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A
project.publication.journalFEBS J
project.publication.doi10.1111/febs.14993
project.publication.pmid31291689
project.publication.published_date2019 Jul 10
project.statusreleased
project.submitted_date2018-05-28
project.released_date2019-07-16
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameStanford Synchrotron Radiation Lightsource (SSRL)
experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
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experiment.sample.molecule[0].long_nameChitinase 1
experiment.sample.molecule[0].short_nameChiAt1
experiment.sample.molecule[0].sequenceG G A Q Q C G S Q A G G A V C P N G L C C S Q F G Y C G S T S P Y C G N G C Q S Q C G G G S S P T P N P P S G G G G G G G G G G S G V G S I I S S S L F D Q I L L H R N D A A C P A H G F Y T Y D A F V A A A N A F S G F A T T G D A D T Q K R E I A A F L A Q T S H E T T G G W P T A P D G P Y S W G Y C F L Q E Q G N P G D Y C V P N D Q W P C A P G K K Y Y G R G P I Q I S Y N Y N Y G P C G N A I G S D L L N N P D L V A S D P T V S F K T A L W F W M T P Q S P K P S C H D V I T G Q W T P S A A D Q A A G R V P G F G V I T N I I N G G V E C G H G S D S R D E D R V G F Y K R Y C D I L G V S Y G D N L D C G N Q S H F
experiment.sample.molecule[0].organismAgave tequilana
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw31.753
experiment.sample.molecule[0].total_mw31.753
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.nameMES 50 mM
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
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experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameClass I chitinase 1 from Agave tequilana
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMexico
experiment.contributor[0].affiliation[0].full_nameIQ, UNAM
experiment.contributor[0].affiliation[0].webpagehttps://www.iquimica.unam.mx/
experiment.contributor[0].contributor_nameYusvel
experiment.contributor[0].contributor_surnameSierra-Gómez
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-04-26
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
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tags[]
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i0_calibration_standard_datanull
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symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDE7
statussuccess
started_at2026-08-11T16:26:57.051392+00:00
finished_at2026-08-11T16:27:05.365153+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDDE7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.",
    "publication": {
      "title": "A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.",
      "author_list": "Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A",
      "journal": "FEBS J",
      "doi": "10.1111/febs.14993",
      "pmid": "31291689",
      "published_date": "2019 Jul 10"
    },
    "status": "released",
    "submitted_date": "2018-05-28",
    "released_date": "2019-07-16"
  },
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          "long_name": "Chitinase 1",
          "short_name": "ChiAt1",
          "sequence": "G G A Q Q C G S Q A G G A V C P N G L C C S Q F G Y C G S T S P Y C G N G C Q S Q C G G G S S P T P N P P S G G G G G G G G G G S G V G S I I S S S L F D Q I L L H R N D A A C P A H G F Y T Y D A F V A A A N A F S G F A T T G D A D T Q K R E I A A F L A Q T S H E T T G G W P T A P D G P Y S W G Y C F L Q E Q G N P G D Y C V P N D Q W P C A P G K K Y Y G R G P I Q I S Y N Y N Y G P C G N A I G S D L L N N P D L V A S D P T V S F K T A L W F W M T P Q S P K P S C H D V I T G Q W T P S A A D Q A A G R V P G F G V I T N I I N G G V E C G H G S D S R D E D R V G F Y K R Y C D I L G V S Y G D N L D C G N Q S H F",
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View the complete manifest.json source
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