SASDDE7

Class I chitinase 1 from Agave tequilana

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Class I chitinase 1 from Agave tequilana

浓度— – 0.23 缓冲液 / pHMES 50 mM / 6.0
实验温度15.0 设备 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
波长1.127 nm曝光1.0 s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Chitinase 1
查看序列
GGAQQCGSQAGGAVCPNGLCCSQFGYCGSTSPYCGNGCQSQCGGGSSPTPNPPSGGGGGGGGGGSGVGSIISSSLFDQILLHRNDAACPAHGFYTYDAFVAAANAFSGFATTGDADTQKREIAAFLAQTSHETTGGWPTAPDGPYSWGYCFLQEQGNPGDYCVPNDQWPCAPGKKYYGRGPIQISYNYNYGPCGNAIGSDLLNNPDLVASDPTVSFKTALWFWMTPQSPKPSCHDVITGQWTPSAADQAAGRVPGFGVITNIINGGVECGHGSDSRDEDRVGFYKRYCDILGVSYGDNLDCGNQSHF
proteinAgave tequilana——–—monomer分子数 131.753 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1444[3]0.0131603–0.418793 1/A含误差列缺失 05sasbdb/entries/e7/sasdde7/source/SASDDE7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.66—Å
i0Guinier257.541.72—
i0P(r)257.2——
mwExperimental31.7—kDa
mwGuinier I(0)30.52.0kDa
mwPorod32.5—kDa
porod_volumePorod52.0—ų
rgGuinier2.5510.029Å
rgP(r)2.61—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称MES 50 mM缓冲液浓度—
pH6.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-04-26储存 / 测量温度4.0 / 15.0
曝光时间1.0帧数—
波长1.127样品-探测器距离1.78
光源X-ray synchrotron探测器Rayonix MX225-HE
机构 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q 范围0.132 – 4.188样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDDE7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.author_listSierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A
project.publication.journalFEBS J
project.publication.doi10.1111/febs.14993
project.publication.pmid31291689
project.publication.published_date2019 Jul 10
project.statusreleased
project.submitted_date2018-05-28
project.released_date2019-07-16
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experiment.instrument.detector.resolutionnull
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experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
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experiment.sample.molecule[0].long_nameChitinase 1
experiment.sample.molecule[0].short_nameChiAt1
experiment.sample.molecule[0].sequenceG G A Q Q C G S Q A G G A V C P N G L C C S Q F G Y C G S T S P Y C G N G C Q S Q C G G G S S P T P N P P S G G G G G G G G G G S G V G S I I S S S L F D Q I L L H R N D A A C P A H G F Y T Y D A F V A A A N A F S G F A T T G D A D T Q K R E I A A F L A Q T S H E T T G G W P T A P D G P Y S W G Y C F L Q E Q G N P G D Y C V P N D Q W P C A P G K K Y Y G R G P I Q I S Y N Y N Y G P C G N A I G S D L L N N P D L V A S D P T V S F K T A L W F W M T P Q S P K P S C H D V I T G Q W T P S A A D Q A A G R V P G F G V I T N I I N G G V E C G H G S D S R D E D R V G F Y K R Y C D I L G V S Y G D N L D C G N Q S H F
experiment.sample.molecule[0].organismAgave tequilana
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw31.753
experiment.sample.molecule[0].total_mw31.753
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.nameMES 50 mM
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
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experiment.sample.purity_methodnull
experiment.sample.nameClass I chitinase 1 from Agave tequilana
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMexico
experiment.contributor[0].affiliation[0].full_nameIQ, UNAM
experiment.contributor[0].affiliation[0].webpagehttps://www.iquimica.unam.mx/
experiment.contributor[0].contributor_nameYusvel
experiment.contributor[0].contributor_surnameSierra-Gómez
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-04-26
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
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experiment.wavelength1.127
experiment.sample_detector_distance1.78
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tags[]
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i0_calibration_standard_datanull
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symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDE7
statussuccess
started_at2026-08-11T16:26:57.051392+00:00
finished_at2026-08-11T16:27:05.365153+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDDE7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.",
    "publication": {
      "title": "A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.",
      "author_list": "Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A",
      "journal": "FEBS J",
      "doi": "10.1111/febs.14993",
      "pmid": "31291689",
      "published_date": "2019 Jul 10"
    },
    "status": "released",
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    "released_date": "2019-07-16"
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          "long_name": "Chitinase 1",
          "short_name": "ChiAt1",
          "sequence": "G G A Q Q C G S Q A G G A V C P N G L C C S Q F G Y C G S T S P Y C G N G C Q S Q C G G G S S P T P N P P S G G G G G G G G G G S G V G S I I S S S L F D Q I L L H R N D A A C P A H G F Y T Y D A F V A A A N A F S G F A T T G D A D T Q K R E I A A F L A Q T S H E T T G G W P T A P D G P Y S W G Y C F L Q E Q G N P G D Y C V P N D Q W P C A P G K K Y Y G R G P I Q I S Y N Y N Y G P C G N A I G S D L L N N P D L V A S D P T V S F K T A L W F W M T P Q S P K P S C H D V I T G Q W T P S A A D Q A A G R V P G F G V I T N I I N G G V E C G H G S D S R D E D R V G F Y K R Y C D I L G V S Y G D N L D C G N Q S H F",
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查看完整 manifest.json 原文
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