SASDDT8

Neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5)

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5)

Concentration2.3 – 9.3 Buffer / pHphosphate buffered saline / 7.4
Experimental temperature25.0 Facility / beamlineESRF / ID14-3
Wavelength0.093 nmExposure10.0 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Insulin-like peptide 5
View sequence
NSLRACGPALMDMLRVACPNGFNSMFAKGVVDSCCRKSCSFSTLRAYCDS
proteinDrosophila melanogasterE7BBS4—–—monomerMolecule count 15.385 kDa
Neural/ectodermal development factor IMP-L2
View sequence
MEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKFTKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVRVRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTEKTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMGNYKCIARNVVGKDTADTFVYPVLNEED
proteinDrosophila melanogasterQ09024—–—monomerMolecule count 129.895 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11270[3]0.0644891–6.10763 1/nmHas uncertainty columnMissing 00sasbdb/entries/t8/sasddt8/source/SASDDT8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.0—nm
i0Guinier44.770.03arbitrary
mwExperimental30.0—kDa
mwGuinier I(0)30.0—kDa
porod_volumePorod55.0—nm³
rgGuinier2.590.04nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer namephosphate buffered salineBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2011-11-20Storage / measurement temperature5.0 / 25.0
Exposure time10.0Frames10
Wavelength0.093Sample-to-detector distance2.43
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / ID14-3 · Grenoble, France
q range0.064 – 6.108Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDDT8
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.
project.publication.titleStructures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.
project.publication.author_listRoed NK, Viola CM, Kristensen O, Schluckebier G, Norrman M, Sajid W, Wade JD, Andersen AS, Kristensen C, Ganderton TR, Turkenburg JP, De Meyts P, Brzozowski AM
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-018-06192-3
project.publication.pmid30242155
project.publication.published_date2018 Sep 21
project.statusreleased
project.submitted_date2018-06-21
project.released_date2018-12-03
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameID14-3
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameInsulin-like peptide 5
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceNSLRACGPALMDMLRVACPNGFNSMFAK GVVDSCCRKSCSFSTLRAYCDS
experiment.sample.molecule[0].organismDrosophila melanogaster
experiment.sample.molecule[0].uniprot_codeE7BBS4
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFRSVIPVLLFLIPLLLSAQAANSLRACGPALMDMLRVACPNGFNSMFAKRGTLGLFDYE DHLADLDSSESHHMNSLSSIRRDFRGVVDSCCRKSCSFSTLRAYCDS
experiment.sample.molecule[0].mw5.385
experiment.sample.molecule[0].total_mw5.385
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameNeural/ectodermal development factor IMP-L2
experiment.sample.molecule[1].short_nameImp-L2 IBP
experiment.sample.molecule[1].sequenceMEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF TKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR VRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE KTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG NYKCIARNVVGKDTADTFVYPVLNEED
experiment.sample.molecule[1].organismDrosophila melanogaster
experiment.sample.molecule[1].uniprot_codeQ09024
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF TKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR VRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE KTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG NYKCIARNVVGKDTADTFVYPVLNEED
experiment.sample.molecule[1].mw29.895
experiment.sample.molecule[1].total_mw29.895
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.namephosphate buffered saline
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNeural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressDenmark
experiment.contributor[0].affiliation[0].full_nameNovo Nordisk A/S
experiment.contributor[0].affiliation[0].webpagehttps://www.novonordisk.com/
experiment.contributor[0].contributor_nameMathias
experiment.contributor[0].contributor_surnameNorrman
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2011-11-20
experiment.storage_temperature5.0
experiment.cell_temperature25.0
experiment.exposure_time10.0
experiment.number_of_frames10
experiment.wavelength0.093
experiment.sample_detector_distance2.43
experiment.concentration_min2.3
experiment.concentration_max9.3
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.064
experiment.s_max6.108
experiment.total_exposure_timenull
experiment.seccolumnnull
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pddf_softwarenull
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i0_calibration_standardnull
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experiment_descriptionSynchrotron SAXS data from solutions of neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5) in phosphate buffered saline, pH 7.4 were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.093 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 9.3 mg/ml were measured at 25°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unitarbitrary
experimental_mw30.0
experimental_mw_errornull
guinier_i0_mw30.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0null
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guinier_i0_error0.03
pddf_rgnull
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guinier_rg_error0.04
pddf_dmax9.0
pddf_dmax_errornull
porod_volume55.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first26
guinier_point_last65
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDT8_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:33 field values
Field pathRaw value
codeSASDDT8
statussuccess
started_at2026-08-11T16:00:11.279148+00:00
finished_at2026-08-11T16:00:18.090106+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDDT8",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.",
    "publication": {
      "title": "Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.",
      "author_list": "Roed NK, Viola CM, Kristensen O, Schluckebier G, Norrman M, Sajid W, Wade JD, Andersen AS, Kristensen C, Ganderton TR, Turkenburg JP, De Meyts P, Brzozowski AM",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-018-06192-3",
      "pmid": "30242155",
      "published_date": "2018 Sep 21"
    },
    "status": "released",
    "submitted_date": "2018-06-21",
    "released_date": "2018-12-03"
  },
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          "short_name": null,
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  "experiment_description": "Synchrotron SAXS data from solutions of neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5) in phosphate buffered saline, pH 7.4 were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.093 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 9.3 mg/ml were measured at 25°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
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  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
View the complete manifest.json source
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