SASDE68

Two dimers of antitoxin GraA in complex with the grta operator

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Two dimers of antitoxin GraA in complex with the grta operator

Concentration— – 10.0 Buffer / pH50 mM Tris 250 mM NaCl 2 mM TCEP / 8.0
Experimental temperature10.0 Facility / beamlineSOLEIL / SWING
Wavelength0.10332 nmExposure1.5 s × 254

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
GraTA operator region
View sequence
AAATTAACGAATAACGTTAAGCATTCAGCTCATTTTAATTGCTTATTGCAATTCGTAAGTCGAGTA
DNA———–—monomerMolecule count 120.402 kDa
antitoxin GraA, antidote of toxin GraT
View sequence
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA
proteinPseudomonas putidaQ88MI61–99dimerMolecule count 210.905 kDa
atitoxin GraA, antidote of GraT.
View sequence
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVAHHHHHH
proteinPseudomonas putidaQ88MI61–99dimerMolecule count 211.728 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1568[3]0.01306281–0.3347094 1/AHas uncertainty columnMissing 03sasbdb/entries/68/sasde68/source/SASDE68.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.25—Å
i0Guinier0.017131e-05arbitrary
i0P(r)0.017139.1e-06arbitrary
mwExperimental67.055—kDa
mwGuinier I(0)67.055—kDa
mwPorod59.0—kDa
porod_volumePorod94.0—ų
rgGuinier2.920.002Å
rgP(r)2.940.02Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris 250 mM NaCl 2 mM TCEPBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-12-17Storage / measurement temperature10.0 / 10.0
Exposure time1.5Frames254
Wavelength0.10332Sample-to-detector distance1.785
SourceX-ray synchrotronDetectorAVIEX PCCD170170
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.131 – 3.347Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:168 field values
Field pathRaw value
codeSASDE68
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleA dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.
project.publication.titleA dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.
project.publication.author_listTalavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-019-08865-z
project.publication.pmid30814507
project.publication.published_date2019 Feb 27
project.statusreleased
project.submitted_date2018-11-15
project.released_date2019-03-04
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDE68.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDE68.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE68_dat_img.png
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE68_guinier_img.png
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experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGraTA operator region
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceAAATTAACGAATAACGTTAAGCATTCAGCTCAT TTTAATTGCTTATTGCAATTCGTAAGTCGAGTA
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw20.402
experiment.sample.molecule[0].total_mw20.402
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameantitoxin GraA, antidote of toxin GraT
experiment.sample.molecule[1].short_nameGraA
experiment.sample.molecule[1].sequenceMLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA
experiment.sample.molecule[1].organismPseudomonas putida
experiment.sample.molecule[1].uniprot_codeQ88MI6
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last99
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLS ICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA
experiment.sample.molecule[1].mw10.905
experiment.sample.molecule[1].total_mw21.81
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameatitoxin GraA, antidote of GraT.
experiment.sample.molecule[2].short_nameGraA
experiment.sample.molecule[2].sequenceMLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVAHHHHHH
experiment.sample.molecule[2].organismPseudomonas putida
experiment.sample.molecule[2].uniprot_codeQ88MI6
experiment.sample.molecule[2].uniprot_range_first1
experiment.sample.molecule[2].uniprot_range_last99
experiment.sample.molecule[2].oligomerizationdimer
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequenceMLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLS ICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA
experiment.sample.molecule[2].mw11.728
experiment.sample.molecule[2].total_mw23.456
experiment.sample.molecule[2].number_molecules2
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris 250 mM NaCl 2 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTwo dimers of antitoxin GraA in complex with the grta operator
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameVUB
experiment.contributor[0].affiliation[0].addressPleinlaan 2 1050 Brussel
experiment.contributor[0].affiliation[0].full_nameVrije Universiteit Brussel
experiment.contributor[0].affiliation[0].webpagehttp://www.vub.ac.be/
experiment.contributor[0].contributor_nameAriel
experiment.contributor[0].contributor_surnameTalavera
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-12-17
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time1.5
experiment.number_of_frames254
experiment.wavelength0.10332
experiment.sample_detector_distance1.785
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.131
experiment.s_max3.347
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of antitoxin GraA in complex with the grta operator in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 10°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Sample injection concentration: approx. 10 mg/mL; Injection volume: 40 μL. 254 successive 1.500 second frames were collected. The SAXS data measured through the SEC elution profile of the sample were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
tags[]
intensity_unitarbitrary
experimental_mw67.055
experimental_mw_errornull
guinier_i0_mw67.055
guinier_i0_mw_errornull
porod_mw59.0
porod_mw_errornull
pddf_i00.01713
pddf_i0_error9.1e-06
guinier_i00.01713
guinier_i0_error1e-05
pddf_rg2.94
pddf_rg_error0.02
guinier_rg2.92
guinier_rg_error0.002
pddf_dmax10.25
pddf_dmax_errornull
porod_volume94.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last56
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDE68_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDE68
statussuccess
started_at2026-08-11T15:46:36.412801+00:00
finished_at2026-08-11T15:46:43.583643+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDE68",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.",
    "publication": {
      "title": "A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.",
      "author_list": "Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-019-08865-z",
      "pmid": "30814507",
      "published_date": "2019 Feb 27"
    },
    "status": "released",
    "submitted_date": "2018-11-15",
    "released_date": "2019-03-04"
  },
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      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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    "sample": {
      "molecule": [
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          "long_name": "GraTA operator region",
          "short_name": null,
          "sequence": "AAATTAACGAATAACGTTAAGCATTCAGCTCAT\r\nTTTAATTGCTTATTGCAATTCGTAAGTCGAGTA",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
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          "long_name": "antitoxin GraA, antidote of toxin GraT",
          "short_name": "GraA",
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          "organism": "Pseudomonas putida",
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          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLS\nICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA",
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          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
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        },
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          "long_name": "atitoxin GraA, antidote of GraT.",
          "short_name": "GraA",
          "sequence": "MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVAHHHHHH",
          "organism": "Pseudomonas putida",
          "uniprot_code": "Q88MI6",
          "uniprot_range_first": 1,
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          "mw": 11.728,
          "total_mw": 23.456,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
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        "name": "50 mM Tris 250 mM NaCl 2 mM TCEP",
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        "comment": null,
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        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Two dimers of antitoxin GraA in complex with the grta operator",
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      "absorbption": null,
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    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "VUB",
            "address": "Pleinlaan 2 1050 Brussel ",
            "full_name": "Vrije Universiteit Brussel",
            "webpage": "http://www.vub.ac.be/"
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        "contributor_surname": "Talavera",
        "orcid": null
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-12-17",
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    "wavelength": 0.10332,
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  "description": null,
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  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE68_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
View the complete manifest.json source
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