SASDEG2

Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Data type: SASBDB experimental data Status: Published Curve type: Other Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Concentration0.0 – 2.0 Buffer / pH50mM Tris, 150mM NaCl, imidiazole / 7.4
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.099 nmExposure1.0 s × 50

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Mitochondrial import inner membrane translocase subunit TIM9
View sequence
MDALNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFTDCVNDFTTSKLTNKEQTCIMKCSEKFLKHSERVGQRFQEQNAALGQGLGR
proteinSaccharomyces cerevisiaeO74700—–—hexamerMolecule count 610.202 kDa
Mitochondrial import inner membrane translocase subunit TIM10
View sequence
GSFLGFGGGQPQLSSQQKIQAAEAELDLVTDMFNKLVNNCYKKCINTSYSEGELNKNESSCLDRCVAKYFETNVQVGENMQKMGQSFNAAGKF
proteinSaccharomyces cerevisiaeP87108—–—hexamerMolecule count 610.23 kDa
Mitochondrial GTP/GDP carrier protein 1
View sequence
QSGLARLLGSASAGIMEIAVFHPVDTISKRLMSNHTKITSGQELNRVIFRDHFSEPLGKRLFTLFPGLGYAASYKVLQRVYKYGGQPFANEFLNKHYKKDFDNLFGEKTGKAMRSAAAGSLIGIGEIVLLPLDVLKIKRQTNPESFKGRGFIKILRDEGLFNLYRGWGWTAARNAPGSFALFGGNAFAKEYILGLKDYSQATWSQNFISSIVGASSSLIVSAPLDVIKTRIQNRNFDNPESGLRIVKNTLKNEGVTAFFKGLTPKLLTTGPKLVFSFALAQSLIPRFDNLLSKLEHHHHHH
proteinSaccharomyces cerevisiaeP389888–300monomerMolecule count 133.426 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11014[3]0.16957–4.9448 1/nmHas uncertainty columnMissing 09sasbdb/entries/g2/sasdeg2/source/SASDEG2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)16.0—nm
i0Guinier13.90.0095arbitrary
i0P(r)13.97—arbitrary
mwExperimental160.0—kDa
mwPorod160.0—kDa
porod_volumePorod272.0—nm³
rgGuinier4.510.03nm
rgP(r)4.601—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50mM Tris, 150mM NaCl, imidiazoleBuffer concentration—
pH7.4Additives—
Buffer descriptionThe imidiazole concentration varies between 200 mM and 300 mM
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-02-22Storage / measurement temperature20.0 / 20.0
Exposure time1.0Frames50
Wavelength0.099Sample-to-detector distance2.872
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.17 – 4.945Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zip:source record and ZIP contents (15 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:327 field values
Field pathRaw value
codeSASDEG2
statusPublished
type_of_curveOther
angular_unit1/nm
project.titleStructural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.
project.publication.titleStructural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.
project.publication.author_listWeinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
project.publication.journalCell
project.publication.doi10.1016/j.cell.2018.10.039
project.publication.pmid30445040
project.publication.published_date2018 Nov 15
project.statusreleased
project.submitted_date2018-08-13
project.released_date2018-11-21
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDEG2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDEG2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEG2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEG2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEG2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEG2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDEG2.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMitochondrial import inner membrane translocase subunit TIM9
experiment.sample.molecule[0].short_nameTIM9
experiment.sample.molecule[0].sequenceMDALNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFTDCVNDFTTSKLTNKEQTCIMKCSEKFLKHSE RVGQRFQEQNAALGQGLGR
experiment.sample.molecule[0].organismSaccharomyces cerevisiae
experiment.sample.molecule[0].uniprot_codeO74700
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationhexamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMDALNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFTDCVNDFTTSKLTNKEQTCIMKCS EKFLKHSERVGQRFQEQNAALGQGLGR
experiment.sample.molecule[0].mw10.202
experiment.sample.molecule[0].total_mw61.212
experiment.sample.molecule[0].number_molecules6
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameMitochondrial import inner membrane translocase subunit TIM10
experiment.sample.molecule[1].short_nameTIM10
experiment.sample.molecule[1].sequenceGSFLGFGGGQPQLSSQQKIQAAEAELDLVTDMFNKLVNNCYKKCINTSYSEGELNKNESSCLDRCVAKYFETNVQVGENMQKMGQSFNAAGKF
experiment.sample.molecule[1].organismSaccharomyces cerevisiae
experiment.sample.molecule[1].uniprot_codeP87108
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationhexamer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMSFLGFGGGQPQLSSQQKIQAAEAELDLVTDMFNKLVNNCYKKCINTSYSEGELNKNESS CLDRCVAKYFETNVQVGENMQKMGQSFNAAGKF
experiment.sample.molecule[1].mw10.23
experiment.sample.molecule[1].total_mw61.38
experiment.sample.molecule[1].number_molecules6
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameMitochondrial GTP/GDP carrier protein 1
experiment.sample.molecule[2].short_nameGGC1
experiment.sample.molecule[2].sequenceQSGLARLLGSASAGIMEIAVFHPVDTISKRLMSNHTKITSGQELNRVIFRDHFSEPLGKRLFTLFPGLGYAASYKVLQRVYKYGGQPFANEFLNKHYKKDFDNLFGEKTGKAMRSAAAGSLIGIGEIVLLPLDVLKIKRQTNPESFKGRGFIKILRDEGLFNLYRGWGWTAARNAPGSFALFGGNAFAKEYILGLKDYSQATWSQNFISSIVGASSSLIVSAPLDVIKTRIQNRNFDNPESGLRIVKNTLKNEGVTAFFKGLTPKLLTTGPKLVFSFALAQSLIPRFDNLLSKLEHHHHHH
experiment.sample.molecule[2].organismSaccharomyces cerevisiae
experiment.sample.molecule[2].uniprot_codeP38988
experiment.sample.molecule[2].uniprot_range_first8
experiment.sample.molecule[2].uniprot_range_last300
experiment.sample.molecule[2].oligomerizationmonomer
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequenceMPHTDKKQSGLARLLGSASAGIMEIAVFHPVDTISKRLMSNHTKITSGQELNRVIFRDHF SEPLGKRLFTLFPGLGYAASYKVLQRVYKYGGQPFANEFLNKHYKKDFDNLFGEKTGKAM RSAAAGSLIGIGEIVLLPLDVLKIKRQTNPESFKGRGFIKILRDEGLFNLYRGWGWTAAR NAPGSFALFGGNAFAKEYILGLKDYSQATWSQNFISSIVGACSSLIVSAPLDVIKTRIQN RNFDNPESGLRIVKNTLKNEGVTAFFKGLTPKLLTTGPKLVFSFALAQSLIPRFDNLLSK
experiment.sample.molecule[2].mw33.426
experiment.sample.molecule[2].total_mw33.426
experiment.sample.molecule[2].number_molecules1
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.buffer.name50mM Tris, 150mM NaCl, imidiazole
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentThe imidiazole concentration varies between 200 mM and 300 mM
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL
experiment.contributor[0].affiliation[0].addressGrenoble, France
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Grenoble Outstation
experiment.contributor[0].affiliation[0].webpagehttps://www.embl.fr/
experiment.contributor[0].contributor_nameMartha
experiment.contributor[0].contributor_surnameBrennich
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-02-22
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames50
experiment.wavelength0.099
experiment.sample_detector_distance2.872
experiment.concentration_min0.0
experiment.concentration_max2.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.17
experiment.s_max4.945
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].chi_square_value1.358
fits[0].p_value4.5e-05
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fits[1].p_value0.0
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View the complete summary.json source
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      "title": "Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.",
      "author_list": "Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P",
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View the complete manifest.json source
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