SASDEU9

Legionella pneumophila Phosphocholinase AnkX

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2024-02-21T18:33:53.450280+01:00

X-ray exposure time = UNKNOWN. Sample temperature = UNKNOWN.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Legionella pneumophila Phosphocholinase AnkX

浓度— – 8.0 缓冲液 / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
实验温度— 设备 / 束线ESRF / BM29
波长0.09919 nm曝光— s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Phosphocholinase AnkX
查看序列
GVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFWSKENGWRGYESREPGCLNAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGELRTDEPVSFGIPAGRASIKGIEEFLSLVFLTEGGAEFGPGKAGPFGPRFDKNYFKNLNPEQIPDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSYNKEIKTAETLDEKLKIIVKHIRMYEVLHPFRDANGRTFVNNLLNIPLMQQGLPPATFYEPNVFDLYSAEELVVVVKEAIFNTVEIIEQSKRKTPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPEKLHLKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVGKTPAHLAVISGNMAMLDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLSQEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVIQINEPDNSGSSAITLAYKNHKLKIFDELLNSGADISDELLDAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLGSVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNSVLHYVFYSKAENREALANIITEKDKKLINQPNANGNPPLYNAVVVNDLKMATILLEMGARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHQALHEMHTFPSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHTNISSPSKFLKNSDANSILERPFKFKNDLKKELDNNPLIAMAQINDLYVQIKNNRIRTPTGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKFFDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW
proteinLegionella pneumophilaQ5ZXN6—–—monomer分子数 1107.2 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1400[3]0.178784–2.060208 1/nm含误差列缺失 00sasbdb/entries/u9/sasdeu9/source/SASDEU9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)14.7—nm
i0Guinier86.330.1arbitrary
i0P(r)86.76—arbitrary
mwExperimental109.0—kDa
mwPorod90.0—kDa
porod_volumePorod145.0—nm³
rgGuinier4.250.03nm
rgP(r)4.324—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-10-20储存 / 测量温度— / —
曝光时间—帧数—
波长0.09919样品-探测器距离—
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.179 – 2.06样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDEU9
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleLegionella pneumophila effector AnkX
project.publicationnull
project.statusreleased
project.submitted_date2019-01-17
project.released_date2024-01-17
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePhosphocholinase AnkX
experiment.sample.molecule[0].short_nameAnkX
experiment.sample.molecule[0].sequenceGVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFWSKENGWRGYESREPGCL NAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGE LRTDEPVSFGIPAGRASIKGIEEFLSLVFLTEGGAEFGPGKAGPFGPRF DKNYFKNLNPEQIPDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEK ITQSYNKEIKTAETLDEKLKIIVKHIRMYEVLHPFRDANGRTFVNNLLN IPLMQQGLPPATFYEPNVFDLYSAEELVVVVKEAIFNTVEIIEQSKRKT PITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPEKLHLKTQKC LSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG KTPAHLAVISGNMAMLDELIAKKADLSLQDYDGKTALHYAAECGNMQIM GKILKVVLSQEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVI QINEPDNSGSSAITLAYKNHKLKIFDELLNSGADISDELLDAIWARKDK ETLGKIIAKNEKILLNKEAFRIAISLGSVSLVKKFLRAGVDIDIPLTKD KATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNSVLHYVFYSKAENR EALANIITEKDKKLINQPNANGNPPLYNAVVVNDLKMATILLEMGARVD FEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHQALH EMHTFPSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIALSKQY FHLCVKLMKAGAHTNISSPSKFLKNSDANSILERPFKFKNDLKKELDNN PLIAMAQINDLYVQIKNNRIRTPTGYAPKEGVSFFKGKSNDAKAHDEVL SVLKELYDSKLTEMLGNLPGEGLEEIKRSQKFFDGELKLLIKNQDISRK VDKKSIQEAVGTSLKLKW
experiment.sample.molecule[0].organismLegionella pneumophila
experiment.sample.molecule[0].uniprot_codeQ5ZXN6
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFWSKENGWRGYESREPGCLNAALESLCSIA LQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGELRTDEPVSFGIPAGRASIKGIE EFLSLVFLTEGGAEFGPGKAGPFGPRFDKNYFKNLNPEQIPDLAKQIYFDMCKYGHSNTN HFYLAVMKNVDVYLEKITQSYNKEIKTAETLDEKLKIIVKHIRMYEVLHPFRDANGRTFV NNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVVKEAIFNTVEIIEQSKRKTPITLYG YHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPEKLHLKTQKCLSSLNEQYPLHRGAIYL SDPGEIKLLLSNRNESQINQQIEQGAPPIYVGKTPAHLAVISGNMAMLDELIAKKADLSL QDYDGKTALHYAAECGNMQIMGKILKVVLSQEDAIKVLNIKDNHGKTAFHYAAEFGTPEL ISALTTTEVIQINEPDNSGSSAITLAYKNHKLKIFDELLNSGADISDELLDAIWARKDKE TLGKIIAKNEKILLNKEAFRIAISLGSVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSG NPKLVSYLLKKGANTRLTDTSGNSVLHYVFYSKAENREALANIITEKDKKLINQPNANGN PPLYNAVVVNDLKMATILLEMGARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLH KRNSERRNPFHQALHEMHTFPSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIAL SKQYFHLCVKLMKAGAHTNISSPSKFLKNSDANSILERPFKFKNDLKKELDNNPLIAMAQ INDLYVQIKNNRIRTPTGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNL PGEGLEEIKRSQKFFDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW
experiment.sample.molecule[0].mw107.2
experiment.sample.molecule[0].total_mw107.2
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLegionella pneumophila Phosphocholinase AnkX
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-10-20
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_timenull
experiment.number_of_framesnull
experiment.wavelength0.09919
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.179
experiment.s_max2.06
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareGASBOR
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fits[0].models[0].bead_radius1.9
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fits[0].models[0].symmetryP1
fits[0].models[0].commentnull
fits[0].models[0].user84
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fits[0].softwareGASBOR
fits[0].chi_square_value1.05
fits[0].p_value0.0
fits[0].fit_residual_plotSASDEU9_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionX-ray exposure time = UNKNOWN. Sample temperature = UNKNOWN.
experiment_descriptionSynchrotron SAXS data from solutions of phosphocholinase AnkX in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 camera at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw109.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw90.0
porod_mw_errornull
pddf_i086.76
pddf_i0_errornull
guinier_i086.33
guinier_i0_error0.1
pddf_rg4.324
pddf_rg_errornull
guinier_rg4.25
guinier_rg_error0.03
pddf_dmax14.7
pddf_dmax_errornull
porod_volume145.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last26
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEU9_datloglog_img.png
symmetrynull
last_modified2024-02-21T18:33:53.450280+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEU9
statussuccess
started_at2026-08-11T16:22:13.366464+00:00
finished_at2026-08-11T16:22:21.619623+00:00
source_last_modified2024-02-21T18:33:53.450280+01:00
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查看完整 summary.json 原文
{
  "code": "SASDEU9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Legionella pneumophila effector AnkX",
    "publication": null,
    "status": "released",
    "submitted_date": "2019-01-17",
    "released_date": "2024-01-17"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDEU9.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEU9.dat",
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          "oligomerization": "monomer",
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      "name": "Legionella pneumophila Phosphocholinase AnkX",
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      "absorbption": null,
      "deuteration": null,
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        "affiliation": [
          {
            "short_name": null,
            "address": "Cachan, France",
            "full_name": "CNRS UMR8113, ENS Paris-Saclay",
            "webpage": "http://lbpa.ens-paris-saclay.fr/"
          }
        ],
        "contributor_name": "Wenhua",
        "contributor_surname": "Zhang",
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    "concentration_unit": null,
    "date": "2017-10-20",
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          "user": 84
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  "last_modified": "2024-02-21T18:33:53.450280+01:00",
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}
查看完整 manifest.json 原文
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