Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

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    "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
    "publication": {
      "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
      "author_list": "Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.09.007",
      "pmid": "31601460",
      "published_date": "2019 Oct 5"
    },
    "status": "released",
    "submitted_date": "2019-01-17",
    "released_date": "2019-10-14"
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        "type": "CCD",
        "name": "AVIEX PCCD170170",
        "resolution": null
      },
      "name": "SOLEIL",
      "city": "Saint-Aubin",
      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Cytohesin-2; ARNO truncation mutant",
          "short_name": "ARNO-CC",
          "sequence": "TLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFL\r\nYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQAL\r\nRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCY\r\nVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEE\r\nLLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGR\r\nVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRK\r\nPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEK\r\nDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP",
          "organism": "Homo sapiens",
          "uniprot_code": "Q99418",
          "uniprot_range_first": 58,
          "uniprot_range_last": 400,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQ\nRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREE\nLNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQ\nSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRN\nEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEP\nRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPT\nQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP",
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
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      "purity_method": null,
      "name": "Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant",
      "ext_coefficient": null,
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      "absorbption": null,
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      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Cachan, France",
            "full_name": "CNRS UMR8113, ENS Paris-Saclay",
            "webpage": "http://lbpa.ens-paris-saclay.fr/"
          }
        ],
        "contributor_name": "Wenhua",
        "contributor_surname": "Zhang",
        "orcid": null
      }
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    "date": "2015-11-25",
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    "cell_temperature": 15.0,
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    "wavelength": 0.1033,
    "sample_detector_distance": 1.8,
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions ofthe cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured at 15°C. One 1.5 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "estimated_volume_error": null,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
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