SASDEV9

Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

浓度— – 8.0 缓冲液 / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
实验温度15.0 设备 / 束线SOLEIL / SWING
波长0.1033 nm曝光1.5 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Cytohesin-2; ARNO truncation mutant
查看序列
TLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
proteinHomo sapiensQ9941858–400monomer分子数 139.861 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11106[3]0.0–0.62027221301 1/A含误差列缺失 00sasbdb/entries/v9/sasdev9/source/SASDEV9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.89—Å
i0Guinier0.030411e-051/cm
i0P(r)0.03045—1/cm
mwExperimental41.5—kDa
mwPorod39.0—kDa
porod_volumePorod63.0—ų
rgGuinier2.7450.02Å
rgP(r)2.77—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-11-25储存 / 测量温度15.0 / 15.0
曝光时间1.5帧数1
波长0.1033样品-探测器距离1.8
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.062 – 6.125样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDEV9
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.author_listDas S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.09.007
project.publication.pmid31601460
project.publication.published_date2019 Oct 5
project.statusreleased
project.submitted_date2019-01-17
project.released_date2019-10-14
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experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameCytohesin-2; ARNO truncation mutant
experiment.sample.molecule[0].short_nameARNO-CC
experiment.sample.molecule[0].sequenceTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFL YKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQAL RQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCY VLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEE LLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGR VKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRK PNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEK DEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ99418
experiment.sample.molecule[0].uniprot_range_first58
experiment.sample.molecule[0].uniprot_range_last400
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw39.861
experiment.sample.molecule[0].total_mw39.861
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-25
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.5
experiment.number_of_frames1
experiment.wavelength0.1033
experiment.sample_detector_distance1.8
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.062
experiment.s_max6.125
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIN
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fits[0].p_value0.006663
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fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions ofthe cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured at 15°C. One 1.5 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw41.5
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw39.0
porod_mw_errornull
pddf_i00.03045
pddf_i0_errornull
guinier_i00.03041
guinier_i0_error1e-05
pddf_rg2.77
pddf_rg_errornull
guinier_rg2.745
guinier_rg_error0.02
pddf_dmax9.89
pddf_dmax_errornull
porod_volume63.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last69
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEV9_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEV9
statussuccess
started_at2026-08-11T16:22:41.460940+00:00
finished_at2026-08-11T16:22:51.141417+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDEV9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
    "publication": {
      "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
      "author_list": "Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.09.007",
      "pmid": "31601460",
      "published_date": "2019 Oct 5"
    },
    "status": "released",
    "submitted_date": "2019-01-17",
    "released_date": "2019-10-14"
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          "long_name": "Cytohesin-2; ARNO truncation mutant",
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      "name": "Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant",
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      "contrast": null,
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    },
    "contributor": [
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          {
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            "address": "Cachan, France",
            "full_name": "CNRS UMR8113, ENS Paris-Saclay",
            "webpage": "http://lbpa.ens-paris-saclay.fr/"
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        ],
        "contributor_name": "Wenhua",
        "contributor_surname": "Zhang",
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    "concentration_unit": null,
    "date": "2015-11-25",
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  "intensities_log_log_plot": "SASDEV9_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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