SASDEW9

Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Concentration— – 10.0 Buffer / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
Experimental temperature15.0 Facility / beamlineESRF / BM29
Wavelength0.993 nmExposure1.0 s × 1500

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Cytohesin-2 ARF nucleotide-binding site opener
View sequence
MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
proteinHomo sapiensQ994181–400dimerMolecule count 246.6 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11030[3]0.0723579–4.95184 1/nmHas uncertainty columnMissing 029sasbdb/entries/w9/sasdew9/source/SASDEW9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)19.65—nm
i0Guinier43.990.067arbitrary
i0P(r)44.95—arbitrary
mwExperimental94.0—kDa
mwPorod90.0—kDa
porod_volumePorod145.0—nm³
rgGuinier4.750.1nm
rgP(r)5.107—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-06-23Storage / measurement temperature15.0 / 15.0
Exposure time1.0Frames1500
Wavelength0.993Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.072 – 4.952Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDEW9
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.author_listDas S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.09.007
project.publication.pmid31601460
project.publication.published_date2019 Oct 5
project.statusreleased
project.submitted_date2019-01-17
project.released_date2019-10-14
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.sample.molecule[0].long_nameCytohesin-2 ARF nucleotide-binding site opener
experiment.sample.molecule[0].short_nameARNO
experiment.sample.molecule[0].sequenceMEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK KFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNL VQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL ERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRR WFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGN HMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ99418
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last400
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQ RNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREE LNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQ STDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRN EPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEP RGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPT QEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
experiment.sample.molecule[0].mw46.6
experiment.sample.molecule[0].total_mw93.2
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCytohesin-2; ARF nucleotide-binding site opener, ARNO
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-06-23
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
experiment.number_of_frames1500
experiment.wavelength0.993
experiment.sample_detector_distancenull
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experiment.concentration_max10.0
experiment.sample_volumenull
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pddf_softwareATSAS GNOM
pddf_software_version5.0
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descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of the cytohesin-2; ARF nucleotide-binding site opener, ARNO, in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.993 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 1500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw94.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw90.0
porod_mw_errornull
pddf_i044.95
pddf_i0_errornull
guinier_i043.99
guinier_i0_error0.067
pddf_rg5.107
pddf_rg_errornull
guinier_rg4.75
guinier_rg_error0.1
pddf_dmax19.65
pddf_dmax_errornull
porod_volume145.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last43
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEW9_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDEW9
statussuccess
started_at2026-08-11T16:23:09.628261+00:00
finished_at2026-08-11T16:23:18.326247+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDEW9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
    "publication": {
      "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
      "author_list": "Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.09.007",
      "pmid": "31601460",
      "published_date": "2019 Oct 5"
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    "status": "released",
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          "long_name": "Cytohesin-2 ARF nucleotide-binding site opener",
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View the complete manifest.json source
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