SASDEW9

Cytohesin-2; ARF nucleotide-binding site opener, ARNO

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Cytohesin-2; ARF nucleotide-binding site opener, ARNO

浓度— – 10.0 缓冲液 / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
实验温度15.0 设备 / 束线ESRF / BM29
波长0.993 nm曝光1.0 s × 1500

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Cytohesin-2 ARF nucleotide-binding site opener
查看序列
MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP
proteinHomo sapiensQ994181–400dimer分子数 246.6 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11030[3]0.0723579–4.95184 1/nm含误差列缺失 029sasbdb/entries/w9/sasdew9/source/SASDEW9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)19.65—nm
i0Guinier43.990.067arbitrary
i0P(r)44.95—arbitrary
mwExperimental94.0—kDa
mwPorod90.0—kDa
porod_volumePorod145.0—nm³
rgGuinier4.750.1nm
rgP(r)5.107—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-06-23储存 / 测量温度15.0 / 15.0
曝光时间1.0帧数1500
波长0.993样品-探测器距离—
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.072 – 4.952样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDEW9
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.titleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
project.publication.author_listDas S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.09.007
project.publication.pmid31601460
project.publication.published_date2019 Oct 5
project.statusreleased
project.submitted_date2019-01-17
project.released_date2019-10-14
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
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experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ99418
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last400
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experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw46.6
experiment.sample.molecule[0].total_mw93.2
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
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experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
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experiment.concentration_unitnull
experiment.date2016-06-23
experiment.storage_temperature15.0
experiment.cell_temperature15.0
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of the cytohesin-2; ARF nucleotide-binding site opener, ARNO, in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.993 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 1500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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intensity_unitarbitrary
experimental_mw94.0
experimental_mw_errornull
guinier_i0_mwnull
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pddf_rg_errornull
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pddf_dmax_errornull
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estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last43
pddf_point_firstnull
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i0_calibration_standard_datanull
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symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEW9
statussuccess
started_at2026-08-11T16:23:09.628261+00:00
finished_at2026-08-11T16:23:18.326247+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Single concentration",
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    "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
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      "title": "Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.",
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      "doi": "10.1016/j.str.2019.09.007",
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          "sequence": "MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK\r\nKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNL\r\nVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL\r\nERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRR\r\nWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGN\r\nHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP",
          "organism": "Homo sapiens",
          "uniprot_code": "Q99418",
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          "uniprot_range_last": 400,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQ\nRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREE\nLNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQ\nSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRN\nEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEP\nRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPT\nQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP",
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          "deuteration": null,
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          "molecule_description": null
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        "name": "300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl",
        "concentration_unit": null,
        "comment": null,
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        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Cytohesin-2; ARF nucleotide-binding site opener, ARNO",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
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      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Cachan, France",
            "full_name": "CNRS UMR8113, ENS Paris-Saclay",
            "webpage": "http://lbpa.ens-paris-saclay.fr/"
          }
        ],
        "contributor_name": "Wenhua",
        "contributor_surname": "Zhang",
        "orcid": null
      }
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-06-23",
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          "user": 84
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/GS-4_1_fit_img.png",
      "software": "GASBOR",
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      "p_value": 0.0,
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  "intensities_log_log_plot": "SASDEW9_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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