Cold-active AMS8 lipase in the presence of calcium (without polyhistidine affinity tag)

{
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  "status": "Published",
  "type_of_curve": "Single concentration",
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  "project": {
    "title": "Structural interpretations of a flexible cold-active AMS8 lipase by combining small-angle X-ray scattering and molecular dynamics simulation (SAXS-MD).",
    "publication": {
      "title": "Structural interpretations of a flexible cold-active AMS8 lipase by combining small-angle X-ray scattering and molecular dynamics simulation (SAXS-MD).",
      "author_list": "Yaacob N, Kamonsutthipaijit N, Soontaranon S, Leow TC, Rahman RNZRA, Ali MSM",
      "journal": "Int J Biol Macromol",
      "doi": "10.1016/j.ijbiomac.2022.08.145",
      "pmid": "36029961",
      "published_date": "2022 Aug 24"
    },
    "status": "released",
    "submitted_date": "2018-10-27",
    "released_date": "2021-09-20"
  },
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEX6_kratky_img.png",
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  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEX6_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEX6.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "MAR 345 Image Plate",
        "resolution": null
      },
      "name": "Synchrotron Light Research Institute (SLRI)",
      "city": "Nakhon Ratchasima",
      "country": "Thailand",
      "beamline_name": "BL1.3W",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "LipAMS8",
          "short_name": "AMS8",
          "sequence": ">tr|E1B2U7|E1B2U7_9PSED LipAMS8 OS=Pseudomonas sp. AMS8 OX=882519 PE=4 SV=1\r\nMGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGS\r\nTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQ\r\nVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAF\r\nGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPT\r\nQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVL\r\nPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNR\r\nNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIG\r\nYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS",
          "organism": "Pseudomonas sp. AMS8",
          "uniprot_code": "E1B2U7",
          "uniprot_range_first": 1,
          "uniprot_range_last": 476,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGS\nTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQ\nVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAF\nGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPT\nQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVL\nPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNR\nNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIG\nYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS",
          "mw": 50.019,
          "total_mw": 50.019,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM Tris HCl, 5 mM CaCl2",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Cold-active AMS8 lipase in the presence of calcium (without polyhistidine affinity tag)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Thailand",
            "full_name": "Synchrotron Light Research Institute",
            "webpage": "https://www.slri.or.th"
          }
        ],
        "contributor_name": "Norhayati",
        "contributor_surname": "Yaacob",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-03-06",
    "storage_temperature": 10.0,
    "cell_temperature": 25.0,
    "exposure_time": 600.0,
    "number_of_frames": 1,
    "wavelength": 0.138,
    "sample_detector_distance": 1.7,
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    "concentration_max": 2.0,
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    "flow_rate": null,
    "s_min": 0.214,
    "s_max": 3.742,
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      ],
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEX6_fit1_fixed_fit_img.png",
      "software": "DAMMIF",
      "chi_square_value": 1.921,
      "p_value": 0.033735,
      "fit_residual_plot": "SASDEX6_fit1_fitresiduals_img.png",
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      "software_version": "r10552",
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of cold-active AMS8 lipase (without polyhistidine affinity tag) in 50 mM Tris HCl, 5 mM CaCl2, pH 8 were collected on the BL1.3W SAXS/WAXS beam line at the Synchrotron Light Research Institute (SLRI; Nakhon Ratchasima, Thailand) using a MAR 345 Image Plate detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.138 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 25°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "guinier_i0_mw_error": 0.0,
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  "estimated_volume_error": null,
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  "guinier_point_last": 115,
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  "symmetry": null,
  "last_modified": "2022-08-30T18:12:26.610492+02:00",
  "bragg_peak": []
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