Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.",
    "publication": {
      "title": "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.",
      "author_list": "Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L",
      "journal": "J Am Chem Soc",
      "doi": "10.1021/jacs.9b07837",
      "pmid": "31550880",
      "published_date": "2019 Sep 24"
    },
    "status": "released",
    "submitted_date": "2019-06-22",
    "released_date": "2019-09-30"
  },
  "pddf_data": null,
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    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "DNA-directed RNA polymerase subunit delta",
          "short_name": "RpoE",
          "sequence": "MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK",
          "organism": "Bacillus subtilis",
          "uniprot_code": "P12464",
          "uniprot_range_first": 1,
          "uniprot_range_last": 173,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYT\nDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDE\nDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK",
          "mw": 20.399,
          "total_mw": 20.399,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 6.6,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl",
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      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "MUNI",
            "address": "Brno, Czech Republic",
            "full_name": "Masaryk University;   \tResearch group Structure and Dynamics of Proteins.",
            "webpage": "https://www.muni.cz/"
          }
        ],
        "contributor_name": "Vojtěch",
        "contributor_surname": "Kubáň",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-10-03",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 3.6,
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    "flow_rate": null,
    "s_min": 0.013,
    "s_max": 3.792,
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    "seccolumn": null
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  "experiment_description": "Synchrotron SAXS data from solutions of RNAP in 20 mM phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH 6.6 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.60 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
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  "estimated_volume_error": null,
  "guinier_point_first": 54,
  "guinier_point_last": 136,
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  "symmetry": null,
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  "bragg_peak": []
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