SASDF86

Human Galectin-10 (Tyr69Glu mutant)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human Galectin-10 (Tyr69Glu mutant)

Concentration— – 20.0 Buffer / pH20 mM Hepes 150 NaCl / 7.4
Experimental temperature15.0 Facility / beamlineSOLEIL / SWING
Wavelength0.103 nmExposure1.0 s × 14

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Galectin-10 Tyr69Glu
View sequence
GHMSLLPVPYTEAASLSTGSTVTIKGRPLACFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREEGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR
proteinHomo sapiensQ05315—–—dimerMolecule count 216.613 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11194[3]0.00658806991112–0.608870553646 1/AHas uncertainty columnMissing 00sasbdb/entries/86/sasdf86/source/SASDF86.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.15—Å
i0Guinier0.120254—arbitrary
mwExperimental30.2—kDa
mwPorod28.7—kDa
porod_volumePorod46.0—ų
rgGuinier2.1470.01Å
rgP(r)2.172—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Hepes 150 NaClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2018-02-04Storage / measurement temperature— / 15.0
Exposure time1.0Frames14
Wavelength0.103Sample-to-detector distance1.8
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.066 – 6.089Sample volume / flow rate50.0 / 0.3

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:166 field values
Field pathRaw value
codeSASDF86
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleProtein crystallization promotes type 2 immunity and is reversible by antibody treatment.
project.publication.titleProtein crystallization promotes type 2 immunity and is reversible by antibody treatment.
project.publication.author_listPersson EK, Verstraete K, Heyndrickx I, Gevaert E, Aegerter H, Percier JM, Deswarte K, Verschueren KHG, Dansercoer A, Gras D, Chanez P, Bachert C, Gonçalves A, Van Gorp H, De Haard H, Blanchetot C, Saunders M, Hammad H, Savvides SN, Lambrecht BN
project.publication.journalScience
project.publication.doi10.1126/science.aaw4295
project.publication.pmid31123109
project.publication.published_date2019 May 24
project.statusreleased
project.submitted_date2019-05-10
project.released_date2019-09-13
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experiment.instrument.detector.resolution75.0
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experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGalectin-10 Tyr69Glu
experiment.sample.molecule[0].short_nameGal-10
experiment.sample.molecule[0].sequenceGHMSLLPVPYTEAASLSTGSTVTIKGRPLACFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREEGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ05315
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].total_mw33.226
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Hepes 150 NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman Galectin-10 (Tyr69Glu mutant)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameClement
experiment.contributor[0].contributor_surnameBlanchet
experiment.contributor[0].orcidnull
experiment.contributor[1].affiliation[0].short_namenull
experiment.contributor[1].affiliation[0].addressGent, Belgium
experiment.contributor[1].affiliation[0].full_nameGhent University
experiment.contributor[1].affiliation[0].webpagehttps://www.ugent.be/
experiment.contributor[1].contributor_nameKenneth
experiment.contributor[1].contributor_surnameVerstraete
experiment.contributor[1].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-02-04
experiment.storage_temperaturenull
experiment.cell_temperature15.0
experiment.exposure_time1.0
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experiment.sample_detector_distance1.8
experiment.concentration_minnull
experiment.concentration_max20.0
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experiment.total_exposure_timenull
experiment.seccolumn10
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fits[0].models[0].pdb_link[0].pdb_code6gkt
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i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw30.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
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estimated_volumenull
estimated_volume_errornull
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pddf_point_firstnull
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i0_calibration_standard_datanull
intensities_log_log_plotSASDF86_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDF86
statussuccess
started_at2026-08-11T15:03:47.328759+00:00
finished_at2026-08-11T15:03:57.434452+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
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  "status": "Published",
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  "angular_unit": "1/A",
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    "title": "Protein crystallization promotes type 2 immunity and is reversible by antibody treatment.",
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      "title": "Protein crystallization promotes type 2 immunity and is reversible by antibody treatment.",
      "author_list": "Persson EK, Verstraete K, Heyndrickx I, Gevaert E, Aegerter H, Percier JM, Deswarte K, Verschueren KHG, Dansercoer A, Gras D, Chanez P, Bachert C, Gonçalves A, Van Gorp H, De Haard H, Blanchetot C, Saunders M, Hammad H, Savvides SN, Lambrecht BN",
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View the complete manifest.json source
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