查看完整 summary.json 原文
{
"code": "SASDFC5",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.",
"publication": {
"title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.",
"author_list": "Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K",
"journal": "J Struct Biol",
"doi": "10.1016/j.jsb.2019.09.007",
"pmid": "31550533",
"published_date": "2019 Sep 21"
},
"status": "released",
"submitted_date": "2019-04-08",
"released_date": "2019-11-14"
},
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},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
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"sample": {
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{
"long_name": "Enhanced disease susceptibility",
"short_name": "EDS1",
"sequence": ">sp|Q9SU72|EDS1C_ARATH Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1\r\nMAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\r\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\r\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\r\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\r\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\r\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\r\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\r\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\r\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\r\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\r\nTLPKNHKSHSPLRDYMMDEITDT",
"organism": "Arabidopsis thaliana",
"uniprot_code": "Q9SU72",
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\nTLPKNHKSHSPLRDYMMDEITDT",
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"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
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"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "Enhanced disease susceptibility 1 (EDS1)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Germany",
"full_name": "University of Cologne",
"webpage": "http://uni-koeln.de/"
}
],
"contributor_name": "Martin",
"contributor_surname": "Voss",
"orcid": ""
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2015-05-19",
"storage_temperature": 4.0,
"cell_temperature": 4.0,
"exposure_time": 2.0,
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"wavelength": 0.099,
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"comment": null,
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}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/EDS1komp_fixed_fit_img.png",
"software": "DAMMIN",
"chi_square_value": 0.824,
"p_value": 0.059642,
"fit_residual_plot": "EDS1komp_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFC5_fit1.fir",
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}
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"description": "The data were re-binned to calculate the p(r) profile and corresponding model fit.",
"experiment_description": "Synchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4 mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 72.0,
"experimental_mw_error": null,
"guinier_i0_mw": null,
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"porod_volume": 92.0,
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"estimated_volume": null,
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"guinier_point_first": 23,
"guinier_point_last": 80,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDFC5_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}
查看完整 manifest.json 原文
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