SASDFC5

Enhanced disease susceptibility 1 (EDS1)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2019-12-06T13:07:15.624532+01:00

The data were re-binned to calculate the p(r) profile and corresponding model fit.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Enhanced disease susceptibility 1 (EDS1)

浓度— – 5.0 缓冲液 / pH50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT / 8.0
实验温度4.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光2.0 s × 1495

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Enhanced disease susceptibility
查看序列
>SP|Q9SU72|EDS1C_ARATHPROTEINEDS1OS=ARABIDOPSISTHALIANAOX=3702GN=EDS1PE=1SV=1MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDT
proteinArabidopsis thalianaQ9SU72—–—monomer分子数 171.689 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.03636141–4.938935 1/nm含误差列缺失 076sasbdb/entries/c5/sasdfc5/source/SASDFC5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.53—nm
i0Guinier151.870.47arbitrary
i0P(r)152.6—arbitrary
mwExperimental72.0—kDa
mwPorod58.0—kDa
porod_volumePorod92.0—nm³
rgGuinier3.090.02nm
rgP(r)3.08—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-05-19储存 / 测量温度4.0 / 4.0
曝光时间2.0帧数1495
波长0.099样品-探测器距离2.867
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 4.939样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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summarysasbdb/entries/c5/sasdfc5/source/summary.jsondownloaded872183ed8dfe2bd9256dd6500492a483565e110eb3b41623d2e5baa6991bb0fc5be6下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDFC5
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleArabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.
project.publication.titleArabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.
project.publication.author_listVoss M, Toelzer C, Bhandari DD, Parker JE, Niefind K
project.publication.journalJ Struct Biol
project.publication.doi10.1016/j.jsb.2019.09.007
project.publication.pmid31550533
project.publication.published_date2019 Sep 21
project.statusreleased
project.submitted_date2019-04-08
project.released_date2019-11-14
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDFC5.dat
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEnhanced disease susceptibility
experiment.sample.molecule[0].short_nameEDS1
experiment.sample.molecule[0].sequence>sp|Q9SU72|EDS1C_ARATH Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1 MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS FGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT GHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV NFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA NQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA SDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA ALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK ANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE DTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS GSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI TLPKNHKSHSPLRDYMMDEITDT
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeQ9SU72
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS FGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT GHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV NFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA NQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA SDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA ALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK ANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE DTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS GSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI TLPKNHKSHSPLRDYMMDEITDT
experiment.sample.molecule[0].mw71.689
experiment.sample.molecule[0].total_mw71.689
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEnhanced disease susceptibility 1 (EDS1)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGermany
experiment.contributor[0].affiliation[0].full_nameUniversity of Cologne
experiment.contributor[0].affiliation[0].webpagehttp://uni-koeln.de/
experiment.contributor[0].contributor_nameMartin
experiment.contributor[0].contributor_surnameVoss
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-05-19
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames1495
experiment.wavelength0.099
experiment.sample_detector_distance2.867
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.036
experiment.s_max4.939
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDFC5_fit1_model1_img.png
fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versionnull
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fits[0].models[0].model_mw57.855
fits[0].models[0].bead_radius2.6
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user414
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/EDS1komp_fixed_fit_img.png
fits[0].softwareDAMMIN
fits[0].chi_square_value0.824
fits[0].p_value0.059642
fits[0].fit_residual_plotEDS1komp_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe data were re-binned to calculate the p(r) profile and corresponding model fit.
experiment_descriptionSynchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4 mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.
tags[]
intensity_unitarbitrary
experimental_mw72.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw58.0
porod_mw_errornull
pddf_i0152.6
pddf_i0_errornull
guinier_i0151.87
guinier_i0_error0.47
pddf_rg3.08
pddf_rg_errornull
guinier_rg3.09
guinier_rg_error0.02
pddf_dmax10.53
pddf_dmax_errornull
porod_volume92.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first23
guinier_point_last80
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFC5_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDFC5
statussuccess
started_at2026-08-11T14:44:42.957263+00:00
finished_at2026-08-11T14:44:54.655628+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDFC5",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.",
    "publication": {
      "title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.",
      "author_list": "Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K",
      "journal": "J Struct Biol",
      "doi": "10.1016/j.jsb.2019.09.007",
      "pmid": "31550533",
      "published_date": "2019 Sep 21"
    },
    "status": "released",
    "submitted_date": "2019-04-08",
    "released_date": "2019-11-14"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFC5.dat",
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        "name": "Pilatus 1M",
        "resolution": 172.0
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      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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          "long_name": "Enhanced disease susceptibility",
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          "sequence": ">sp|Q9SU72|EDS1C_ARATH Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1\r\nMAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\r\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\r\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\r\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\r\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\r\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\r\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\r\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\r\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\r\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\r\nTLPKNHKSHSPLRDYMMDEITDT",
          "organism": "Arabidopsis thaliana",
          "uniprot_code": "Q9SU72",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Enhanced disease susceptibility 1 (EDS1)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Germany",
            "full_name": "University of Cologne",
            "webpage": "http://uni-koeln.de/"
          }
        ],
        "contributor_name": "Martin",
        "contributor_surname": "Voss",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-05-19",
    "storage_temperature": 4.0,
    "cell_temperature": 4.0,
    "exposure_time": 2.0,
    "number_of_frames": 1495,
    "wavelength": 0.099,
    "sample_detector_distance": 2.867,
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    "s_max": 4.939,
    "total_exposure_time": null,
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          "software": "DAMMIN",
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          "comment": null,
          "user": 414
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/EDS1komp_fixed_fit_img.png",
      "software": "DAMMIN",
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      "p_value": 0.059642,
      "fit_residual_plot": "EDS1komp_fitresiduals_img.png",
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  "description": "The data were re-binned to calculate the p(r) profile and corresponding model fit.",
  "experiment_description": "Synchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4  mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.",
  "tags": [],
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  "guinier_point_first": 23,
  "guinier_point_last": 80,
  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFC5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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