SASDFJ2

Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2020-08-13T15:33:56.548965+02:00

SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 260-264

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)

浓度— – 2.4 缓冲液 / pH50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME) / 7.5
实验温度23.0 设备 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
波长0.1 nm曝光1.0 s × 500

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Cell wall synthesis protein Wag31
查看序列
MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQELAAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNTAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKADALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSNADAGGFDQFNRGKN
proteinMycobacterium tuberculosisP9WMU1—–—unknown分子数 —29.98 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1946[3]0.00554855–0.402288 1/A含误差列缺失 0221sasbdb/entries/j2/sasdfj2/source/SASDFJ2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
i0Guinier27.75—arbitrary
mwExperimental29.97—kDa
rgGuinier10.14—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-01-29储存 / 测量温度23.0 / 23.0
曝光时间1.0帧数500
波长0.1样品-探测器距离2.54
光源X-ray synchrotron探测器Rayonix MX225-HE
机构 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q 范围0.055 – 4.023样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/j2/sasdfj2/source/SASDFJ2.outnot_listed——下载查看原文件
sascifsasbdb/entries/j2/sasdfj2/source/SASDFJ2.sascifdownloaded565896209b4a629ce44269724c5996077bc00fa4cf1c0d8fa228995cd1670e2689875下载查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(1 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDFJ2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleHigher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.
project.publication.titleHigher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.
project.publication.author_listChoukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
project.publication.journalJ Struct Biol
project.publication.doi10.1016/j.jsb.2019.107429
project.publication.pmid31778770
project.publication.published_date2019 Nov 25
project.statusreleased
project.submitted_date2019-01-30
project.released_date2020-01-09
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameRayonix MX225-HE
experiment.instrument.detector.resolutionnull
experiment.instrument.nameStanford Synchrotron Radiation Lightsource (SSRL)
experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameCell wall synthesis protein Wag31
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN
experiment.sample.molecule[0].organismMycobacterium tuberculosis
experiment.sample.molecule[0].uniprot_codeP9WMU1
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationunknown
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQATQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN
experiment.sample.molecule[0].mw29.98
experiment.sample.molecule[0].total_mw29.98
experiment.sample.molecule[0].number_molecules0
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameCSIR
experiment.contributor[0].affiliation[0].addressChandigarh, India
experiment.contributor[0].affiliation[0].full_nameCSIR-Institute of Microbial Technology
experiment.contributor[0].affiliation[0].webpagehttp://www.imtech.res.in/
experiment.contributor[0].contributor_nameKomal
experiment.contributor[0].contributor_surnameChoukate
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-01-29
experiment.storage_temperature23.0
experiment.cell_temperature23.0
experiment.exposure_time1.0
experiment.number_of_frames500
experiment.wavelength0.1
experiment.sample_detector_distance2.54
experiment.concentration_minnull
experiment.concentration_max2.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.055
experiment.s_max4.023
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
descriptionSEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 260-264
experiment_descriptionSynchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw29.97
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
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pddf_i0null
pddf_i0_errornull
guinier_i027.75
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pddf_rgnull
pddf_rg_errornull
guinier_rg10.14
guinier_rg_errornull
pddf_dmaxnull
pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
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guinier_point_first4
guinier_point_last18
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFJ2_datloglog_img.png
symmetrynull
last_modified2020-08-13T15:33:56.548965+02:00
bragg_peak[]
manifest.json:33 个字段值
字段路径原始值
codeSASDFJ2
statussuccess
started_at2026-08-11T13:39:27.134678+00:00
finished_at2026-08-11T13:39:32.865417+00:00
source_last_modified2020-08-13T15:33:56.548965+02:00
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查看完整 summary.json 原文
{
  "code": "SASDFJ2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
    "publication": {
      "title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
      "author_list": "Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B",
      "journal": "J Struct Biol",
      "doi": "10.1016/j.jsb.2019.107429",
      "pmid": "31778770",
      "published_date": "2019 Nov 25"
    },
    "status": "released",
    "submitted_date": "2019-01-30",
    "released_date": "2020-01-09"
  },
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          "short_name": null,
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          "oligomerization": "unknown",
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  "description": "SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak.\r\nSEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 260-264",
  "experiment_description": "Synchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "experimental_mw_error": null,
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  "guinier_i0": 27.75,
  "guinier_i0_error": null,
  "pddf_rg": null,
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  "guinier_rg": 10.14,
  "guinier_rg_error": null,
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  "pddf_dmax_error": null,
  "porod_volume": null,
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  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 4,
  "guinier_point_last": 18,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFJ2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-08-13T15:33:56.548965+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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